Establishment and analysis of the HPLC fingerprint for Yingde black tea NO.9 produced in different seasons
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摘要: 采用高效液相色谱法（HPLC）对大叶种红茶英红九号的春、夏、秋三季茶产品进行测定，对所得HPLC色谱图中的相对保留时间、相对峰面积、共有峰进行了分析。结果表明，HPLC色谱法具有良好的精密度，各样品色谱峰的相对保留时间稳定，可作为指纹图谱的相对时间，并确定了7个共有峰。以各色谱峰的峰面积为因子，用相关系数、聚类分析等方法构建了数字化的英红九号红茶HPLC指纹图谱。在本研究的实验范围内，该方法在区分不同季节生产的英红九号红茶、以及英红九号红茶与其他品种红茶的差异性等方面具有可行性。
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关键词:
- 聚类分析
Abstract: High performance liquid chromatography (HPLC) was used to measure large tea cultivate Yingde black tea NO.9 produced in spring, summer and autumn; the relative retention time, relative peak area and common peaks were analyzed according to the HPLC chromatogram. The results showed that HPLC method had high precision; and the relative retention time of chromatographic peak of each sample was stable, which could be used as the relative time of fingerprint; and seven common peaks were confirmed. with the application of the peak area of each chromatographic peak as a factor, digitized HPLC fingerprint of the Yingde black tea NO.9 was established by the correlation coefficient and cluster analysis method. In this research scope this method was feasible in discrimination of the Yingde black tea NO.9 produced in different seasons and the difference between Yingde black tea NO.9 and other black teavarieties.-
Keywords:
- cluster analysis
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[1]石尧清.红茶新品种——五岭红、英红九号[J].广东农业科学, 1991, 1(1):17-17
[2]宁井铭, 张正竹, 方世辉, 等.指纹图谱技术及其在茶叶品质控制中的应用[J].中国茶叶加工, 2009, 3(3):39-41
[3]金惠淑.茶叶品质化学和仪器鉴定研究[D]. 杭州: 浙江大学, 2007.
[4]刘继平.指纹图谱质控技术对实现中药现代化的作用和意义[J].陕西中医学院学报, 2006, 29(3):61-64
[5]高俊.黄山毛峰茶指纹图谱的初步研究[D]. 安徽: 安徽农业大学, 2008.
[6]郝志龙, 金心怡, 江丽萍, 等.化学指纹图谱在茶叶品质鉴定与控制上的作用[J].亚热带农业研究, 2009, 5(1):60-63
[7]罗一帆, 郭振飞, 许旋, 等.广东岭头单枞茶高效液相色谱指纹图谱的研究[J].食品科学, 2006, 26(4):206-209
[8]王建程.浙江和福建绿茶中微量元素的聚类分析研究[J].中国测试技术, 2007, 33(3):126-130
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