Preparation of the recombinant mung bean LBBI and its role in protection of mice from radiation
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摘要: 目的: 研究重组绿豆LBBI蛋白是否对小鼠辐射损伤具有防护作用。方法: 60只BALB/c小鼠分为3组(n=20)，即生理盐水组、重组LBBI注射组和安多霖灌胃组。60 Co射线源对小鼠进行一次性全身照射，照射剂量为6.5 Gy。60 Co照射半小时后，对各组小鼠分别注射生理盐水、LBBI（50.0 mg/(kgd)）和灌胃给药安多霖（3 g/(kgd)），每隔24 h给药一次，连续给药一周，观察小鼠成活率及外周血象恢复的情况。结果: 与生理盐水组小鼠相比，重组LBBI组和安多霖组小鼠的30 d存活率明显增高（P0.01），平均生存时间延长（P0.01），小鼠外周血红细胞、白细胞、血小板数目明显增加（P0.05）。结论: 重组LBBI蛋白具有防止辐射损伤的作用。
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关键词:
- 抗辐射
Abstract: Objective: To confirm whether the recombinant mung bean LBBI protein has the protective effect of anti-radiation injury for mice. Methods: 60 BALB/c mice were randomly divided into normal saline control, recombinant LBBI and, anduolin group, all of which were irradiated with 60 Co at 6.5 Gy for whole bod. Half an hour latter, the mice in every group received intravenous injection with normal saline and LBBI(50.0 mg/(kgd) )respectively, and intragastric administration with anduolin(3 g/(kgd) for first time. These treatments were carried out for every 24 h for one week. Then, the survival rate and peripheral hemogram recovery of mice were observed. Results: Compared to normal saline group, the 30 days survival rate of group mice injected with LBBI protein was increased significantly （P0.01）,and its average survival time was prolonged（P0.01）. We also confirmed that the number of red blood cell, white blood cells and platelets in LBBI group mice wereincreased significantly than that of normal saline group（P0.05）. Conclusions: The recombinant LBBI can protect mice from radiation injury.-
Keywords:
- anti-radiation
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[1]汪磊，朱波，王国泽，等.肽和蛋白抗辐射损伤作用研究进展[J].广东农业科学, 2012, 0(18):233-236 [2]Metreveli NO，Jariashvili KK，Namicheishvili LO，et al.UV-vis and FT-IR spectra of ultraviolet irradiated collagen in the presence of antioxidant ascorbic acid[J].Ecotoxicol Environ Saf, 2010, 73(3):448-455 [3]Almeida IF，Pinto AS，Monteiro C，et al．Protective effect of C.sativa leaf extract against UV mediated-DNA damage in a human keratinocyte cell line[J].J Photochem Photobiol B, 2015, 0(144):28-34 [4]Sedla?ková E，Bábelová A，Kozics K，et al.Ultraviolet?A?radiation?potentiates the cytotoxic and genotoxic?effects?of 7 H-dibenzo[c,g] carbazole and its methyl derivatives[J].Environ Mol Mutagen, 2015, 56(4):388-403 [5]Matsumura Y，Ananthaswamy HN.Toxic effects of ultraviolet radiation on the skin[J].Toxicology Applied Pharmacol, 2004, 195(3):298-308 [6]Ataman OU，Sambrook SJ，Wilks C，et al.The clinical development of molecularly targeted agents in combination with?radiation therapy: a?pharmaceuticalperspective[J].Int J Radiat Oncol Biol Phys, 2012, 84(4):e447-54 [7]van Den Hout R，Pouw M，Gruppen H，et al.Inactivation Kinetics Study of the?Kunitz?Soybean?Trypsin Inhibitor?and the?Bowman-Birk?Inhibitor[J].J Agric Food Chem, 1998, 46(1):281-285 [8] Dittmann K，L?ffler H，Bamberg M，et al.Bowman-Birk?proteinase?inhibitor?(BBI)? modulates?radiosensitivity?and?radiation-induced?differentiation?of?humanfibroblasts?in?culture[J].Radiother Oncol, 1995, 34(2):137-143 [9]Costa HM，Freitas Junior AC，Amaral IP，et al.Metal-sensitive and thermostable trypsin from the crevalle jack (Caranx hippos) pyloric caeca: purification and characterization[J].Chemistry Central Journal, 2013, 0(7):0-8 [10].．Radiother Oncol，1995，34(2)：137-143． [11]. Costa HM，Freitas Junior AC，Amaral IP，et al．Metal-sensitive and thermostable trypsin from the crevalle jack (Caranx hippos) pyloric caeca: purification and characterization[J]．Chemistry Central Journal，2013，7：0-8． [12]Scott CJ，Taggart CC.Biologic protease inhibitors as novel therapeutic agents[J].Biochimie, 2010, 92(11):1681-1688 [13]Rengan R，Mick R，Pryma D，et al.A phase I trial of the HIV?protease inhibitor?nelfinavir with concurrent chemoradiotherapy for unresectable stage IIIAIIIB non-small cell lung cancer: a report of toxicities and clinical response[J].J Thorac Oncol, 2012, 7(4):709-715 [14]Qi RF，Song ZW，Chi CW.Structural features and molecular evolution of Bowman-Birk protease inhibitors and their potential application[J].Acta Biochim Biophys Sin, 2005, 37(5):283-292 [15]Hu Z，Xing Y，Qian Y，et al.Anti-radiation?damage?effect of polyethylenimine as a toll-like receptor 5 targeted agonist[J].J Radiat Res, 2013, 54(2):243-250 [16]Shao LJ，Luo Y，Zhou DH.Hematopoietic Stem Cell Injury Induced by Ionizing Radiation[J].Antioxid Redox Signal, 2014, 20(9):1447-1462
[1]汪磊，朱波，王国泽，等.肽和蛋白抗辐射损伤作用研究进展[J].广东农业科学, 2012, 0(18):233-236 [2]Metreveli NO，Jariashvili KK，Namicheishvili LO，et al.UV-vis and FT-IR spectra of ultraviolet irradiated collagen in the presence of antioxidant ascorbic acid[J].Ecotoxicol Environ Saf, 2010, 73(3):448-455 [3]Almeida IF，Pinto AS，Monteiro C，et al．Protective effect of C.sativa leaf extract against UV mediated-DNA damage in a human keratinocyte cell line[J].J Photochem Photobiol B, 2015, 0(144):28-34 [4]Sedla?ková E，Bábelová A，Kozics K，et al.Ultraviolet?A?radiation?potentiates the cytotoxic and genotoxic?effects?of 7 H-dibenzo[c,g] carbazole and its methyl derivatives[J].Environ Mol Mutagen, 2015, 56(4):388-403 [5]Matsumura Y，Ananthaswamy HN.Toxic effects of ultraviolet radiation on the skin[J].Toxicology Applied Pharmacol, 2004, 195(3):298-308 [6]Ataman OU，Sambrook SJ，Wilks C，et al.The clinical development of molecularly targeted agents in combination with?radiation therapy: a?pharmaceuticalperspective[J].Int J Radiat Oncol Biol Phys, 2012, 84(4):e447-54 [7]van Den Hout R，Pouw M，Gruppen H，et al.Inactivation Kinetics Study of the?Kunitz?Soybean?Trypsin Inhibitor?and the?Bowman-Birk?Inhibitor[J].J Agric Food Chem, 1998, 46(1):281-285 [8] Dittmann K，L?ffler H，Bamberg M，et al.Bowman-Birk?proteinase?inhibitor?(BBI)? modulates?radiosensitivity?and?radiation-induced?differentiation?of?humanfibroblasts?in?culture[J].Radiother Oncol, 1995, 34(2):137-143 [9]Costa HM，Freitas Junior AC，Amaral IP，et al.Metal-sensitive and thermostable trypsin from the crevalle jack (Caranx hippos) pyloric caeca: purification and characterization[J].Chemistry Central Journal, 2013, 0(7):0-8 [10].．Radiother Oncol，1995，34(2)：137-143． [11]. Costa HM，Freitas Junior AC，Amaral IP，et al．Metal-sensitive and thermostable trypsin from the crevalle jack (Caranx hippos) pyloric caeca: purification and characterization[J]．Chemistry Central Journal，2013，7：0-8． [12]Scott CJ，Taggart CC.Biologic protease inhibitors as novel therapeutic agents[J].Biochimie, 2010, 92(11):1681-1688 [13]Rengan R，Mick R，Pryma D，et al.A phase I trial of the HIV?protease inhibitor?nelfinavir with concurrent chemoradiotherapy for unresectable stage IIIAIIIB non-small cell lung cancer: a report of toxicities and clinical response[J].J Thorac Oncol, 2012, 7(4):709-715 [14]Qi RF，Song ZW，Chi CW.Structural features and molecular evolution of Bowman-Birk protease inhibitors and their potential application[J].Acta Biochim Biophys Sin, 2005, 37(5):283-292 [15]Hu Z，Xing Y，Qian Y，et al.Anti-radiation?damage?effect of polyethylenimine as a toll-like receptor 5 targeted agonist[J].J Radiat Res, 2013, 54(2):243-250 [16]Shao LJ，Luo Y，Zhou DH.Hematopoietic Stem Cell Injury Induced by Ionizing Radiation[J].Antioxid Redox Signal, 2014, 20(9):1447-1462
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