Abstract: Let U=Tri(A,M,B) be a triangular algebra. A bilinear map # is called a generalized biderivation if it is a generalized derivation with repect to both arguments. In this paper, by using of operator theory methods, we provide the relational characterizations of every generalized biderivation on triangular algebra U. On this basis, We obtained a new form of generalized biderivation. Generalizes the notion of generalized biderivation to a more general case.