绿豆胰蛋白酶抑制剂BBI诱导肺腺癌A549细胞凋亡的研究

基金项目:

国家自然科学基金面上项目;新世纪人才

详细信息

RESEARCH OF APOPTOSIS IN HUAMN LUNG ADENOCARCINOMA CELL LINE A549 INDUCED BY MUNG BEAN TRYSIN INHIBITOR BBI

摘要: 研究了绿豆胰蛋白酶抑制剂BBI对人肺腺癌A549细胞增殖抑制作用和对肺腺癌A549细胞凋亡的影响.采用MTT法检测绿豆BBI对人肺癌A549细胞的增殖抑制作用；流式细胞仪检测和光镜观察绿豆BBI诱导A549细胞凋亡；Western-Blot检测凋亡蛋白Caspase3表达情况.结果显示：绿豆BBI对人肺腺癌A549细胞的增殖有明显抑制作用；绿豆BBI可以促使人肺腺癌A549细胞出现明显的染色质浓缩和凋亡小体，诱导人肺腺癌A549细胞的凋亡.说明绿豆BBI能抑制人肺腺癌A549细胞增殖、诱导人肺腺癌?A549细胞凋亡，实验为绿豆BBI抗肿瘤效应提供了依据.
- 细胞凋亡
Abstract: To evaluate the cell growth inhibition and apoptosis induced by mung bean BBI in A549，we used MTT assay to detect the effect of BBI on cell proliferation. The cell apoptosis was measured by flow cytometer and the morphological changes were observed under microscope. We applied Western-Blot to test the expression level of Caspase3. The results showed that Mung bean BBI could inhibit the cell proliferation of A549.BBI could induce apoptotic changes in A549, such as chromatin condensation and the development of apoptosis bodies. The results indicated that Mung bean BBI could inhibit cell proliferation and induce apoptosis in A549.This provides experimental basis for antitumor activity of BBI.
- apoptosis