Screening, Isolation and biodegradation ability of a Cefradine Degrading Strain
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摘要: 从抗生素生产厂污水排放口取得菌种，并在实验室中驯化、筛选出能以头孢拉定为唯一碳源生长的好氧菌群，经过平板分离纯化得到3株菌种。进行了各菌种以0，5，10，15，20，25mgL-1头孢拉定为唯一碳源的4d生长情况试验，同时进行了10mgL-1头孢拉定中生长4d的菌种生长及降解初步试验。结果表明，经过12代的驯化，筛选出的好氧混合菌群CDB，在底物浓度为0-25 mgL－1时， 4d内均有最佳的生长和降解能力，而分离纯化得到的单株菌中，CDB3菌株的生长情况及其降解能力均较强。各菌种的生长情况与其降解能力具有对应关系。Abstract: An aerobic mixed culture strains CDB that can degrad Cefradine were enriched from wastewater discharge port. CDB could can use Cefradine as sole carbon source for growth. 3 pure single strains were isolated and cultured. The 4d degradation test in 0，5，10，15，20，25mgL-1 Cefradine were done and results showed that the all of the bacterial densities reached the peak after 4 days and slowed down when cultured in 10-25mgL-1 Cefradine. Results also showed that in the test of the degradation ability in 10mgL-1 Cefradine , the density of the mixed culture strains CDB had the highest degradation ability than the single strains. In the 3 single strains, the CDB3 had higher bacterium density and higher degradation ability than the other 2 stains. Strain which grow well in the 10mgL-1 Cefradine has higher degradation ability.
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Keywords:
- Cefradine /
- mixed culture strains /
- single strain /
- microbiodegradation
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