Abstract: To understand the temporal and spatial changes of ecosystem service value (ESV) and their drivers in the Niyang River Basin, based on remote sensing data in 1990, 2000, 2010 and 2020, the spatial information of land use change in 30 m grid was obtained. The spatial-temporal changes in land use and ESV and its driving forces in the Niyang River basin in recent 30 years were analyzed with dynamic degree, equivalent factor approach and geodetector. The results showed that: (1) From 1990 to 2020, the area of construction land and cropland increased to twice their original size, the area of grassland increased irregularly, while the area of the other land decreased. The three main types of land use in the Niyang River basin are grassland, unused land and forest. (2) ESV was higher in the eastern area than that in the western area, the ESV of the Niyang River Basin in 1990, 2000, 2010, and 2020 was RMB 43 295.5 million, RMB 43 295.5 million, RMB 39 043.8 million, and RMB 39 043.8 million, respectively, the change of ESV first underwent a stability phase, subsequently shifting to a decline phase, and finally reaching a slight decline phase. (3) In 2020, a list in the descending order of the ESV as follows: water regulation (RMB 6 379.6 million), biodiversity (RMB 6 069.7 million), soil conservation (RMB 5 692.1 million), climate regulation (RMB 5 077.2 million), gas regulation (RMB 5 014.0 million), disturbance regulation (RMB 4 212.9 million), esthetical scenery (RMB 3 077.1 million), raw material (RMB 2 714.8 mi-llion), food production (RMB 809.9 million); From 1990 to 2020, food production service value continued to improve, while the value of all other types of second service diminished. (4) Distance from the main stream and elevation contribute to the change of ESV is above 10%, which is the main reason for the change. Annual average temperature, fixed assets investment, urbanization rate and annual precipitation are secondary factors. Population increase and urbanization have caused land-use changes in the Niyang River Basin, indirectly affecting changes in the value of ecosystem services. With the active promotion of the protection and construction of the ecological security in the Tibet Autonomous Region, the decline in the ecosystem service value of the Niyang River Basin has been curbed.