一种可富集Fe<sup>3+</sup>的含芘荧光探针

王莎; 黄子杰; 肖盈希; 卢博; 唐健; 马立军

doi:10.6054/j.jscnun.2020091

一种可富集Fe³⁺的含芘荧光探针

华南师范大学化学学院，广州 510006

基金项目:

广东省自然科学基金项目 2015A030313392

广州市科技计划项目 201804010465

详细信息

通讯作者:
马立军，副教授，Email:mlj898021@scnu.edu.cn

中图分类号: O65
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出版历程
- 收稿日期: 2019-09-24
- 网络出版日期: 2021-01-04
- 刊出日期: 2020-12-24

A Pyrene-containing Fluorescent Probe with Enriching Fe³⁺

School of Chemistry, South China Normal University, Guangzhou 510006, China

摘要

摘要: 以芘为荧光发射基团、以邻氨基苯甲酸基团为识别基团，设计、合成了一种对Fe³⁺特异性响应的荧光探针芘甲酰邻氨基苯甲酸(PAA).利用氢核磁共振波谱、质谱表征其结构，通过紫外-可见吸收光谱以及荧光发射光谱研究其对Fe³⁺的响应性能.结果表明：PAA对Fe³⁺具有高选择性、高抗干扰性的荧光响应.随着Fe³⁺浓度的升高，探针的荧光强度逐渐减弱，同时伴随大量Fe³⁺螯合物沉淀的析出，从而使Fe³⁺从体系中富集出来，且在4倍浓度干扰离子存在的体系中，PAA对Fe³⁺的识别能力几乎不受影响.因此，PAA在实际环境应用中，具有较大的潜力和应用价值.
- 荧光探针 /
- 芘 /
- Fe³⁺ /
- 环境应用 /
- 富集
Abstract: A fluorescent probe (pyrene-formyl-anthranilic acid, PAA) with specific response to Fe³⁺ was designed and synthesized using pyrene as a fluorescent group and anthranilic acid group as a receptor. The structure was characterized with hydrogen nuclear magnetic resonance spectroscopy and mass spectrometry. Visible absorption spectra and fluorescence emission spectra were used to study the response of Fe³⁺. The results showed that PAA had high selectivity and high anti-interference fluorescence response to Fe³⁺. With the increase of Fe³⁺ concentration, the probe's fluorescence intensity was gradually weakened, and a large amount of iron ion chelate precipitated and so that it could be removed from the system. In the system where 4 times of interfering ions existed, the ability of PAA to recognize Fe³⁺ was hardly affected. Therefore PAA has a great potential for practical application.
- fluorescent probe /
- pyrene /
- Fe³⁺ /
- environmental application /
- enrichment

HTML全文

图 1 PAA的合成路线

Figure 1. The synthesis route of PAA

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图 2 PAA的¹H NMR及质谱图

Figure 2. The ¹H NMR and ESI-MS spectra of PAA

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图 3 PAA溶液中加入不同浓度Fe³⁺时的紫外-可见吸收光谱和荧光发射光谱

注：图B中插图为在波长为365 nm的紫外灯照射下，探针分子PAA对Fe³⁺响应的照片，其中a溶液c(PAA)=20.0 μmol/L, b溶液中c(PAA)=20.0 μmol/L，c(Fe³⁺)=120.0 μmol/L.

Figure 3. The fluorescence emission spectra and UV-Vis spectra of PAA upon addition of various amounts of Fe³⁺

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图 4 不同金属离子浓度对PAA荧光发射峰强度的影响及其干扰性

注：图A中c(PAA)=20.0 μmol/L, 发射峰中心波长为495 nm.图B为在PAA(20.0 μmol/L)水溶液中，单独添加Fe³⁺浓度(30.0 μmol/L)及同时添加Fe³⁺(30.0 μmol/L)和竞争离子(150.0 μmol/L)时溶液荧光发射强度的相对变化.

Figure 4. The effects of different metal ion concentrations on the intensity of PAA fluorescence emission peaks and their interference

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图 5 不同离子探针比例对PAA荧光发射峰强度的影响及其在最佳结合模式下的拟合曲线

注：图A中PAA与Fe³⁺在水溶液中的总浓度为20.0 μmol/L; 最佳结合模式n(PAA) : n(Fe³⁺)=2:1.

Figure 5. The effect of different ion probe ratios on the intensity of PAA fluorescence emission and its fitting in the best binding mode

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