Preparation of Methylene Blue-Bound DNA Films by Self-standing Casting Methods
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摘要: 基于亚甲基蓝（MB）与DNA间的静电作用与嵌入作用，运用自铸膜法在载玻片上制备了蓝色的MB-DNA复合膜，并应用紫外可见光谱法、荧光光谱法和扫描电镜等方法对复合膜进行了表征，同时研究了DNA-MB复合膜的成膜机制.结果表明，MB能嵌入诱导DNA的凝聚，形成均匀的MB-DNA复合膜，而膜中的DNA对MB起着分散剂的作用，使膜中多聚MB分散成单体和二聚的形式，显示出单体和二聚体的光吸收特性.该研究结果为DNA介导的功能光电器件的构筑提供了有力的支撑.
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关键词:
- 自铸膜法
Abstract: A blue composite film is assembled on a glass substrate using a self-standing casting method based on the electrostatic attraction and intercalation of methylene blue (MB) with DNA. The resulting cast film is characterized by means of UV-visible absorption spectroscopy, fluorescence spectroscopy and scanning electron microscopy, and its casting mechanism mediated by DNA is further explored. The results suggest that MB can intercalate into DNA and simultaneously induce the condensation of DNA to form a uniform MB-DNA composite film. The aggregated forms of MB can be disassociated into monomer or dimer forms based on the dispersion of DNA, revealed by their photoabsorption properties. This present study provides a powerful basis for constructing DNA-mediated optoelectronic functional devices.-
Keywords:
- Self-standing Cast Film Method
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[6]Yang W, Mehmet O, Brynn H D, Justin G J.Evidence for the direct interaction between Methylene Blue and guanine using DNA-modified carbon paste electrodes[J].Electroanalysis, 2002, 14(18):1299-1302
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[10]Lutterman D A, Chouai A, Liu Y, et al.Intercalation is not required for DNA light-switch behavior[J].Journal of the American Chemical Society, 2008, 10(4):1163-70
[11]Patil K, Pawar R, Talap P.Self-aggregation of Methylene Blue in aqueous medium and aqueous solutions of Bu4NBr and urea[J].Phys chem chem. phys, 2000, 2(19):4313-4317
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[13]Reichmann M E, Rice S A, Thomas C A, Doty P.A further examination of the molecular weight and size of desoxypentose nucleic acid[J].Journal of the American Chemical Society, 1954, 76(11):3047-3053
[14]Chen M J, Li H, Shao J Y, et al.Preparation of a [Ru(bpy)2(dppz)]2+-intercalated DNA cast film using a self-standing method and its luminescence tuning by Cu2+ ions and EDTA[J].Inorganic Chemistry, 2011, 50(6):2043-5
[15]邓琳, 逯丹凤, 祁志美.光波导分光光谱技术研究染料分子在玻璃表面的吸附特性[J].物理化学学报, 2009, 25(12):2481-2487
[16]Deng L, Lu D F, Qi Z M.Adsorption of dye molecules onto a glass surface studied by optical waveguide spectroscopy[J].Acta Physico–Chinica Sinica, 2009, 25(12):2481-2487
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[18]Huang X Y, Meng X G, Zhang Y, et al.Synthesis and properties on promoting DNA condensation of polybenzimidazole Mn(II) complexes[J].Chemical Journal of Chinese University, 2012, 33(6):1151-1157
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[20]Tsunoda K, Umemura T, Ueno H, Okuno E, Akaiwa H.Adsorption of methylene blue onto silylated silica surfaces,studied using visible attenuated total reflection spectroscopy with a slab optical waveguide[J].Applied Spectroscopy, 2003, 57(10):1273-7
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[24]Xu W J.Synchronous fluorescence determination of DNA based on the interaction between methylene blue and DNA[J].Analytica Chimica Acta, 2007, 587(2):187-193
[25]Hackl E V, Kornilova S V, Blagoi Y P.DNA structural transitions induced by divalent metal ions in aqueous solutions[J].Int J Biol Macromol, 2005, 35(4):175-191
[26]Marino T, Toscano M, Russo N, Grand A.Gas-phase interaction between DNA and RNA bases and copper(II) ion: A density functional study[J].International Journal of Quantum Chemistry, 2004, 98(4):347-354
[27]王新莹, 纪鸣, 李志果, 等.金属离子与相互作用的研究进展[J].分析化学学报, 2005, 21(5):557-562
[28]Wang X Y, Ji M, Li Z G, et al.Progress of research on the interactions between DNA and metal ions[J].Journal of Analytical Science, 2005, 21(5):557-562
[1]Hajian R, Tavakol M.Interaction of anticancer drug methotrexate with DS-DNA analyzed by spectroscopic and electrochemical methods[J].E-Journal of Chemistry, 2012, 9(1):471-480
[2]Felice D L, Sun J, Liu R H.A modified methylene blue assay for accurate cell counting[J].Journal of Functional Foods, 2009, 1(1):109-118
[3]Zhu N, Zhang A, Wang Q, et al.Electrochemical detection of DNA hybridization using Methylene Blue and electro-deposited zirconia thin films gold electrodes[J].Analytica Chimica Acta, 2004, 510(2):163-168
[4]He X, Wu X, Wang K.Methylene Blue-encapsulated phosphonate-terminated silica nanoparticles for simultaneous in vivo imaging and photodynamic therapy[J].Biomaterials, 2009, 30(29):5601-5609
[5]Elaheh F, Lilia C, Gothelf K V, Ferapontova E E.DNA Interactions with a Methylene Blue redox indicator depend on the DNA length and are sequence specific[J].Analyst, 2010, 135(6):1443-1448
[6]Yang W, Mehmet O, Brynn H D, Justin G J.Evidence for the direct interaction between Methylene Blue and guanine using DNA-modified carbon paste electrodes[J].Electroanalysis, 2002, 14(18):1299-1302
[7]Zhang L Z, Tang G Q.The binding properties of photosensitizer Methylene Blue to herring sperm DNA: a spectroscopic study[J].Journal of Photochemistry & Photobiology B Biology, 2004, 74(2-3):119-125
[8] Hammond R W, Oana H, Schwinefus J J, et al.Capillary electrophoresis of supercoiled and linear DNA in dilute hydroxyethyl cellulose solution[J].[J].Analytical Chemistry, 1997, 69(6):1192-1196
[9] Vijayanathan V, Thomas T, Thomas T J.DNA nanoparticles and development of DNA delivery vehicles for gene therapy[J]. [J].Biochemistry, 2002, 41(48):14085-14094
[10]Lutterman D A, Chouai A, Liu Y, et al.Intercalation is not required for DNA light-switch behavior[J].Journal of the American Chemical Society, 2008, 10(4):1163-70
[11]Patil K, Pawar R, Talap P.Self-aggregation of Methylene Blue in aqueous medium and aqueous solutions of Bu4NBr and urea[J].Phys chem chem. phys, 2000, 2(19):4313-4317
[12] Marmur J.A procedure for the isolation of deoxyribonucleic acids from microorganisms[J].[J].Journal of Molecular Biology, 1961, 3(2):208-218
[13]Reichmann M E, Rice S A, Thomas C A, Doty P.A further examination of the molecular weight and size of desoxypentose nucleic acid[J].Journal of the American Chemical Society, 1954, 76(11):3047-3053
[14]Chen M J, Li H, Shao J Y, et al.Preparation of a [Ru(bpy)2(dppz)]2+-intercalated DNA cast film using a self-standing method and its luminescence tuning by Cu2+ ions and EDTA[J].Inorganic Chemistry, 2011, 50(6):2043-5
[15]邓琳, 逯丹凤, 祁志美.光波导分光光谱技术研究染料分子在玻璃表面的吸附特性[J].物理化学学报, 2009, 25(12):2481-2487
[16]Deng L, Lu D F, Qi Z M.Adsorption of dye molecules onto a glass surface studied by optical waveguide spectroscopy[J].Acta Physico–Chinica Sinica, 2009, 25(12):2481-2487
[17]黄雪英, 孟祥高, 张妍, 等.多苯并咪唑锰配合物的合成及对凝聚的促进作用[J].高等学校化学学报, 2012, 33(6):1151-1157
[18]Huang X Y, Meng X G, Zhang Y, et al.Synthesis and properties on promoting DNA condensation of polybenzimidazole Mn(II) complexes[J].Chemical Journal of Chinese University, 2012, 33(6):1151-1157
[19] Chen L L, Chao H, Zhao Q W, Zheng X L, Li H.Photoluminescence quenching of [Ru(bpy)2(atatp)]2+ bound to a condensed DNA matrix[J]. [J].Journal of Inorganic Biochemistry, 2015, 154:1-6
[20]Tsunoda K, Umemura T, Ueno H, Okuno E, Akaiwa H.Adsorption of methylene blue onto silylated silica surfaces,studied using visible attenuated total reflection spectroscopy with a slab optical waveguide[J].Applied Spectroscopy, 2003, 57(10):1273-7
[21]Ghosh M, Nath S, Hajra A, Sinha S.Fluorescence self-quenching of tetraphenylporphyrin in liquid medium[J].Journal of Luminescence, 2013, 141(17):87-92
[22]吴海霞, 康敬万, 李志锋, 等.亚甲基蓝与相互作用的电化学研究[J].分析测试学报, 2006, 25(4):1-5
[23]Wu H X, Kang J W, Li Z F, et al.Electrochemical investigation on interaction between DNA with methylene blue[J].Journal of Instrumental Analysis, 2006, 25(4):1-5
[24]Xu W J.Synchronous fluorescence determination of DNA based on the interaction between methylene blue and DNA[J].Analytica Chimica Acta, 2007, 587(2):187-193
[25]Hackl E V, Kornilova S V, Blagoi Y P.DNA structural transitions induced by divalent metal ions in aqueous solutions[J].Int J Biol Macromol, 2005, 35(4):175-191
[26]Marino T, Toscano M, Russo N, Grand A.Gas-phase interaction between DNA and RNA bases and copper(II) ion: A density functional study[J].International Journal of Quantum Chemistry, 2004, 98(4):347-354
[27]王新莹, 纪鸣, 李志果, 等.金属离子与相互作用的研究进展[J].分析化学学报, 2005, 21(5):557-562
[28]Wang X Y, Ji M, Li Z G, et al.Progress of research on the interactions between DNA and metal ions[J].Journal of Analytical Science, 2005, 21(5):557-562 -
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