Analysis of the OsMYB80 Downstream Genes in Regulating Pollen Fertility

LI Zhiai; HUANG Xiaoyan; WANG Changjian; LI Yonghong; YANG Xiaohuai; LI Pingdong; QIU Shijun; CHEN Shujing; WU Jianxin; XU Chunjue; TANG Xiaoyan; CHEN Zisheng

doi:10.6054/j.jscnun.2024010

Journal of South China Normal University (Natural Science Edition) > 2024 > 56(1): 78-91. > DOI: 10.6054/j.jscnun.2024010

LI Zhiai, HUANG Xiaoyan, WANG Changjian, LI Yonghong, YANG Xiaohuai, LI Pingdong, QIU Shijun, CHEN Shujing, WU Jianxin, XU Chunjue, TANG Xiaoyan, CHEN Zisheng. Analysis of the OsMYB80 Downstream Genes in Regulating Pollen Fertility[J]. Journal of South China Normal University (Natural Science Edition), 2024, 56(1): 78-91. DOI: 10.6054/j.jscnun.2024010

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Analysis of the OsMYB80 Downstream Genes in Regulating Pollen Fertility

1.
School of Life Sciences/Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, South China Normal University, Guangzhou 510631, China
2.
Shenzhen Institute of Molecular Crop Design, Shenzhen 518107, China
3.
Shenzhen Agricultural Technology Promotion Center, Shenzhen 518055, China

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Received Date: December 04, 2022
Available Online: April 29, 2024

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Abstract

Abstract

OsMYB80 is an important transcription factor for anther and pollen development in rice. However, its downstream target genes need to be further studied. In this study, 24 candidate genes were selected from the genes that may be directly regulated by OsMYB80. The 24 candidate genes may be involved in signal transduction, ubiquitination modification, vesicle transport, and unknown function, and their expression patterns were verified by qRT-PCR. Seven of these genes (LOC_Os06g11930, LOC_Os09g26470, LOC_Os01g04409, LOC_Os06g35160, LOC_Os06g11135, LOC_Os05g13650, LOC_Os01g14880) were mutated using the CRISPR/Cas9 genome editing technology. I₂-KI staining of pollen and seed setting analyses were performed on the knockout mutants. None of the seven gene knockout mutants were found to significantly affect plant fertility, indicating that these genes were not key for pollen fertility. These results also suggested that some of the OsMYB80-regulated genes might be functionally redundant or play a minor role in the anther and pollen development.
- MYB80,
- rice,
- pollen,
- sterility

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References (33)

References

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