| Citation: |
CAO He, SHI Liying, GUO Xiulei, YU Dayong. Inhibitory Effect of Usnic Acid on Proliferation of Human Bladder Cancer Cells[J]. Journal of South China Normal University (Natural Science Edition), 2024, 56(1): 72-77. DOI: 10.6054/j.jscnun.2024009
|
In order to investigate the effect of usnic acid on human bladder cancer, cultured human bladder cancer cells T24 and RT4 were treated with different concentrations of usnic acid (12.5, 25, 50, 100, 200 μmol/L) for 24 h and 48 h, respectively, and the proliferation of T24 and RT4 cells was detected by MTT assay. The results showed that intracellular reactive oxygen species (ROS) levels were increased after treatment with usnic acid, and apoptosis of T24 cells was induced through mitochondrial pathway and death receptor pathway: (1)Usnic acid inhibited the proliferation of T24 and RT4 cells in a time-dependent and dose-dependent manner, and the inhibitory rate of usnic acid on T24 cell proliferation was higher. (2)By Hoechst 33342 staining and Annexin V-FITC/PI double staining, apoptotic T24 cells gradually increased with the increase of the concentration of usnic acid. (3)After treatment with usnic acid, the level of intracellular ROS increased, the expression of Bax, cleaved-Caspase-8, cleaved-Caspase-9 and cleaved-Caspase-3 protein increased, the expression of Bcl-2, Caspase-3, Caspase-8 and caspase-9 decreased, and the ratio of Bax protein expression to Bcl-2 protein expression increased.
| [1] |
WITJES J, BRUINS H, CATHOMAS R, et al. European association of urology guidelines on muscle-invasive and metastatic bladder cancer: summary of the 2020 guidelines[J]. European Urology, 2021, 79(1): 82-104. doi: 10.1016/j.eururo.2020.03.055
|
| [2] |
APPLEYARD S E, NIKAPOTA A D. Patient-reported outcomes and health-related quality of life following radiotherapy for bladder cancer[J]. Clinical Oncology, 2021, 33(6): 400-406. doi: 10.1016/j.clon.2021.03.016
|
| [3] |
BROKSA B, STURDÍKOVÁ M, PRÓNAYOVÁ N, et al. (-)-Usnic acid and its derivatives: their inhibition of fungal growth and enzyme activity[J]. Die Pharmazie, 1996, 51(3): 195-196.
|
| [4] |
殷乐章, 李伟. 松萝酸生物活性和毒性研究进展[J]. 辽宁中医药大学学报, 2018, 20(12): 96-100. https://www.cnki.com.cn/Article/CJFDTOTAL-LZXB201812029.htm
YIN Y Z, LI W. Advances in research on the biological activity and toxicity of usnic acid[J]. Journal of Liaoning University of Traditional Chinese Medicine, 2018, 20(12): 96-100. https://www.cnki.com.cn/Article/CJFDTOTAL-LZXB201812029.htm
|
| [5] |
DURAZO F, LASSMAN C, HAN S, et al. Fulminant liver failure due to usnic acid for weight loss[J]. The American Journal of Gastroenterology, 2004, 99(5): 950-952. doi: 10.1111/j.1572-0241.2004.04165.x
|
| [6] |
左舒婷. 松萝酸通过ROS诱导乳腺癌细胞凋亡机制的实验研究[D]. 长春: 吉林大学, 2016.
ZUO S T. Usnic acid induce apoptpsis via an ROS-dependent mitochondrial pathway in human breast cancer cells[D]. Changchun: Jilin University, 2016.
|
| [7] |
耿晓歌. 松萝酸对人胃癌的抗肿瘤作用及机制的体内外研究[D]. 杭州: 浙江大学, 2018.
GENG X G. The mechainism of anti-tumor of usnic acid in human gastric cancer in vitro and in vivo[D]. Hangzhou: Zhejiang University, 2018.
|
| [8] |
KUMAR K, MISHRA J, SINGH R. Usnic acid induces apoptosis in human gastric cancer cells through ROS genera-tion and DNA damage and causes up-regulation of DNA-PKcs and γ-H2A. X phosphorylation[J]. Chemico-biological Interactions, 2020, 315: 1088-1098.
|
| [9] |
SINGH N, NAMBIAR D, KALE R, et al. Usnic acid inhibits growth and induces cell cycle arrest and apoptosis in human lung carcinoma A549 cells[J]. Nutrition and Cancer, 2013, 65(S1): 36-43.
|
| [10] |
KOPARAL A, TÜYLÜ B, TÜRK H. In vitro cytotoxic activities of (+)-usnic acid and (-)-usnic acid on V79, A549, and human lymphocyte cells and their non-genotoxicity on human lymphocytes[J]. Natural Product Research, 2006, 20(14): 1300-1307. doi: 10.1080/14786410601101910
|
| [11] |
CORY S, ADAMS J. The Bcl2 family: regulators of the cellular life-or-death switch[J]. Nature Reviews Can-cer, 2002, 2(9): 647-656. doi: 10.1038/nrc883
|
| [12] |
LI D, LUO Z, CHEN G, et al. Identification of apoptosis-related genes Bcl2 and Bax from yellow catfish Pelteobagrus fulvidraco and their transcriptional responses to waterborne and dietborne zinc exposure[J]. Gene, 2017, 633: 1-8. doi: 10.1016/j.gene.2017.08.029
|
| [13] |
蔡轶, 魏沁, 蔡跃鹏, 等. 表儿茶素没食子酸酯对人鼻咽癌C666-1细胞凋亡的影响[J]. 华南师范大学学报(自然科学版), 2015, 47(4): 103-107. doi: 10.6054/j.jscnun.2015.05.014
CAI Y, WEI Q, CAI Y P, et al. Effect of epicatechin gallate on the apoptosis of human nasopharyngeal carcinoma cell line C666-1[J]. Journal of South China Normal University(Natural Science Edition), 2015, 47(4): 103-107. doi: 10.6054/j.jscnun.2015.05.014
|
| [14] |
AIPIRE A, CHEN Q, CAI S, et al. N-butanol subfraction of Brassica Rapa L. Promotes reactive oxygen species production and induces apoptosis of A549 lung adenocarcinoma cells via mitochondria-dependent pathway[J]. Molecules, 2018, 23(7): 1-12.
|
| [15] |
REDZA-DUTORDOIR M, AVERILL-BATES D A. Activation of apoptosis signalling pathways by reactive oxygen species[J]. Biochimica et Biophysia Acta-Molecular Cell Reseach, 2016, 1863(12): 2977-2992. doi: 10.1016/j.bbamcr.2016.09.012
|
| 1. |
梁冉. 无线射频能量收集与存储系统设计. 无线互联科技. 2023(03): 1-4 .
|
Supported by: Beijing Renhe Information Technology Co., Ltd. 
DownLoad: