The Effect of Accumulated Exercise on Myocardial Collagen Fibers in Insulin Resistant Mice

ZHANG Yuli; SHUAI Xiangyu; LIANG Fei; QI Yuanpu; ZHANG Limei; YAN Xujie; LIANG Weibao; WANG Songtao

doi:10.6054/j.jscnun.2023022

Journal of South China Normal University (Natural Science Edition) > 2023 > 55(2): 73-79. > DOI: 10.6054/j.jscnun.2023022

ZHANG Yuli, SHUAI Xiangyu, LIANG Fei, QI Yuanpu, ZHANG Limei, YAN Xujie, LIANG Weibao, WANG Songtao. The Effect of Accumulated Exercise on Myocardial Collagen Fibers in Insulin Resistant Mice[J]. Journal of South China Normal University (Natural Science Edition), 2023, 55(2): 73-79. DOI: 10.6054/j.jscnun.2023022

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The Effect of Accumulated Exercise on Myocardial Collagen Fibers in Insulin Resistant Mice

School of Physical Education and Sports Science, South China Normal University, Guangzhou 510006, China

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Received Date: November 14, 2021
Available Online: June 13, 2023

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Abstract

Accumulated exercise is an effective means for breaking sedentary behavior and improving metabolic health. To explore the health intervention value of accumulated exercise, the effects of different intensities of accumulated exercise and continuous exercise have been compared on insulin resistance (IR) status, myocardial collagen fibers, and myocardial fibrosis protein expression in mice through long-term exercise. The IR mouse model was induced by feeding 112 C57BL/6J male mice (4 weeks old) with high-fat diet. The IR model mice were intervened with moderate intensity continuous exercise, moderate intensity accumulated exercise and high intensity accumulated exercise for 8 weeks. Oral glucose tolerance test(OGTT) was performed, and the mice were sacrificed after blood was collected from the orbit to extract heart tissue. The ELISA method was used to detect serum Fasting insulin(FINS) level, myocardial tissue collagen Ⅰ(COLⅠ), collagen Ⅲ(COLⅢ) and α-smooth muscle actin (α-SMA) content. The Western blot method was used to detect mouse myocardial tissue transforming growth factor-β1(TGF-β1), Smad3 and connective tissue growth factor(CTGF) protein expression level. The results showed that all three kinds of exercise could improve the IR state and myocardial fibrosis degree of IR mice, the effects of high intensity accumulated exercise and moderate intensity continuous exercise were equivalent, while the effect of mode-rate intensity accumulated exercise was weaker.
- accumulated exercise,
- insulin resistance,
- myocardial collagen fibers,
- TGF-β1/Smad3

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[1]	WILSON J J, BLACKBURN N E, O'REILLY R, et al. Association of objective sedentary behaviour and self-rated health in English older adults[J]. BMC Research Notes, 2019, 12(1): 12/1-6. doi: 10.1186/s13104-019-4144-0
[2]	HENSON J, DUNSTAN D W, DAVIES M J, et al. Sedentary behaviour as a new behavioural target in the prevention and treatment of type 2 diabetes[J]. Diabetes/Metabolism Research and Reviews, 2016, 32(S1): 213-220.
[3]	HAMILTON M T, HAMILTON D G, ZDERIC T W. Se-dentary behavior as a mediator of type 2 diabetes[J]. Medicine and Sport Science, 2014, 60: 11-26.
[4]	LAHJIBI E, HEUDE B, DEKKER J M, et al. Impact of objectively measured sedentary behaviour on changes in insulin resistance and secretion over 3 years in the RISC study: interaction with weight gain[J]. Diabetes & Metabolism, 2013, 39(3): 217-225. doi: 10.3969/j.issn.1006-6187.2013.03.007
[5]	SAUNDERS T J, LAROUCHE R, COLLEY R C, et al. Acute sedentary behaviour and markers of cardiometabolic risk: a systematic review of intervention studies[J]. Journal of Nutrition and Metabolism, 2012, 2012: 712435/1-12.
[6]	HEALY G N, DUNSTAN D W, SALMON J, et al. Television time and continuous metabolic risk in physically active adults[J]. Medicine & Science in Sports & Exercise, 2008, 40(4): 639-645.
[7]	GARDNER B, SMITH L, LORENCATTO F, et al. How to reduce sitting time?A review of behaviour change strategies used in sedentary behaviour reduction interventions among adults[J]. Health Psychology Review, 2016, 10(1): 89-112. doi: 10.1080/17437199.2015.1082146
[8]	PHILLIPS C M, DILLON C B, PERRY I J. Does replacing sedentary behaviour with light or moderate to vigo-rous physical activity modulate inflammatory status in adults?[J]. International Journal of Behavioral Nutrition and Physical Activity, 2017, 14: 138/1-12.
[9]	BAILEY D P, LOCKE C D. Breaking up prolonged sitting with light-intensity walking improves postprandial glycemia, but breaking up sitting with standing does not[J]. Journal of Science and Medicine in Sport, 2015, 18(3): 294-298. doi: 10.1016/j.jsams.2014.03.008
[10]	ERIKSEN L, DAHL-PETERSEN I, HAUGAARD S B, et al. Comparison of the effect of multiple short-duration with single long-duration exercise sessions on glucose homeostasis in type 2 diabetes mellitus[J]. Diabetologia, 2007, 50(11): 2245-2253. doi: 10.1007/s00125-007-0783-0
[11]	MAGUTAH K, MEIRING R, PATEL N B, et al. Effect of short and long moderate-intensity exercises in modifying cardiometabolic markers in sedentary Kenyans aged 50 years and above[J]. BMJ Open Sport & Exercise Medicine, 2018, 4(1): e000316/1-7.
[12]	MURPHY M H, BLAIR S N, MURTAGH E M. Accumulated versus continuous exercise for health benefit a review of empirical studies[J]. Sports Medicine, 2009, 39(1): 29-43. doi: 10.2165/00007256-200939010-00003
[13]	MIYASHITA M. Effects of continuous versus accumulated activity patterns on postprandial triacylglycerol concentrations in obese men[J]. International Journal of Obesity(Lond), 2008, 32(8): 1271-1278. doi: 10.1038/ijo.2008.73
[14]	YAP M C, BALASEKARAN G, BURNS S F. Acute effect of 30 min of accumulated versus continuous brisk walking on insulin sensitivity in young Asian adults[J]. European Journal of Applied Physiology, 2015, 115(9): 1867-1875. doi: 10.1007/s00421-015-3174-0
[15]	MURPHY M, NEVILL A, NEVILLE C, et al. Accumulating brisk walking for fitness, cardiovascular risk, and psychological health[J]. Medicine & Science in Sports & Exercise, 2002, 34(9): 1468-1474.
[16]	MURTAGH E M, BOREHAM C A, NEVILL A, et al. The effects of 60 minutes of brisk walking per week, accumula-ted in two different patterns, on cardiovascular risk[J]. Preventive Medicine, 2005, 41(1): 92-97. doi: 10.1016/j.ypmed.2004.10.008
[17]	范锦勤, 张丽美, 张亚松, 等. 累积运动对肥胖大鼠内脏脂肪组织巨噬细胞极化的影响[J]. 体育学刊, 2018, 25(2): 135-144. https://www.cnki.com.cn/Article/CJFDTOTAL-TYXK201802024.htm FAN J Q, ZHANG L M, ZHANG Y S, et al. Effects of accumulated exercise on the polarization of macrophages in visceral adipose tissue of obese rats[J]. Journal of Physical Education, 2018, 25(2): 135-144. https://www.cnki.com.cn/Article/CJFDTOTAL-TYXK201802024.htm
[18]	HASKELL W L, LEE I M, PATE R R, et al. Physical activity and public health: updated recommendation for adults from the American College of Sports Medicine and the American Heart Association[J]. Circulation, 2007, 116(9): 1081-1093. doi: 10.1161/CIRCULATIONAHA.107.185649
[19]	GELINEAU R R, ARRUDA N L, HICKS J A, et al. The behavioral and physiological effects of high-fat diet and alcohol consumption: sex differences in C57BL6/J mice[J]. Brain & Behavior, 2017, 7(6): e00708/1-17.
[20]	MLINAR B, MARC J, JANEZ A, et al. Molecular mechanisms of insulin resistance and associated diseases[J]. Clinica Chimica Acta, 2007, 375(1/2): 20-35.
[21]	MANN T N, WEBSTER C, LAMBERTS R P, et al. Effect of exercise intensity on post-exercise oxygen consumption and heart rate recovery[J]. European Journal of Applied Physiology, 2014, 114(9): 1809-1820. doi: 10.1007/s00421-014-2907-9
[22]	SMITH J, NAUGHTON L M. The effects of intensity of exercise on excess postexercise oxygen consumption and energy expenditure in moderately trained men and women[J]. European Journal of Applied Physiology and Occupational Physiology, 1993, 67(5): 420-425. doi: 10.1007/BF00376458
[23]	LARSEN I, WELDE B, MARTINS C, et al. High- and moderate-intensity aerobic exercise and excess post-exercise oxygen consumption in men with metabolic syndrome[J]. Scandinavian Journal of Medicine & Science in Sports, 2014, 24(3): e174-e179.
[24]	SZCZEPANIAK L S, DOBBINS R L, METZGER G J, et al. Myocardial triglycerides and systolic function in humans: in vivo evaluation by localized proton spectroscopy and cardiac imaging[J]. Magnetic Resonance in Medicine, 2003, 49(3): 417-423. doi: 10.1002/mrm.10372
[25]	SUN X, PAN H, TAN H, et al. High free fatty acids level related with cardiac dysfunction in obese rats[J]. Diabetes Research and Clinical Practice, 2012, 95(2): 251-259. doi: 10.1016/j.diabres.2011.10.028
[26]	CAVALERA M, WANG J, FRANGOGIANNIS N G. Obesity, metabolic dysfunction, and cardiac fibrosis: pathophysiological pathways, molecular mechanisms, and therapeutic opportunities[J]. Translational Research, 2014, 164(4): 323-335. doi: 10.1016/j.trsl.2014.05.001
[27]	WARBRICK I, RABKIN S W. Hypoxia-inducible factor 1-alpha (HIF-1alpha) as a factor mediating the relationship between obesity and heart failure with preserved ejection fraction[J]. Obesity Reviews, 2019, 20(5): 701-712. doi: 10.1111/obr.12828
[28]	MAHAJAN R, LAU D H, SANDERS P. Impact of obesity on cardiac metabolism, fibrosis, and function[J]. Trends in Cardiovascular Medicine, 2015, 25(2): 119-126.
[29]	VAN PUTTEN S, SHAFIEYAN Y, HINZ B. Mechanical control of cardiac myofibroblasts[J]. Journal of Molecular and Cellular Cardiology, 2016, 93: 133-142.
[30]	NAKATANI T, HONDA E, HAYAKAWA S, et al. Effects of decorin on the expression of alpha-smooth muscle actin in a human myofibroblast cell line[J]. Molecular and Cellular Biochemistry, 2008, 308(1/2): 201-207.
[31]	WANG Q J, USINGER W, NICHOLS B, et al. Cooperative interaction of CTGF and TGF-b in animal models of fibrotic disease[J]. Fibrogenesis & Tissue Repair, 2011, 4(1): 4/1-11.
[32]	TRAVERS J G, KAMAL F A, ROBBINS J, et al. Cardiac fibrosis: the fibroblast awakens[J]. Circulation Research, 2016, 118(6): 1021-1040.
[33]	LEASK A. TGFbeta, cardiac fibroblasts, and the fibrotic response[J]. Cardiovascular Research, 2007, 74(2): 207-212.

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