The Preparation of Compound Hydrolases for Detergent from Excess Activated Sludge

CHEN Mengtao; WANG Junhua; ZHANG Ziming; FAN Xiaoming; NI He; LI Haihang

doi:10.6054/j.jscnun.2021028

Journal of South China Normal University (Natural Science Edition) > 2021 > 53(2): 65-72. > DOI: 10.6054/j.jscnun.2021028

CHEN Mengtao, WANG Junhua, ZHANG Ziming, FAN Xiaoming, NI He, LI Haihang. The Preparation of Compound Hydrolases for Detergent from Excess Activated Sludge[J]. Journal of South China Normal University (Natural Science Edition), 2021, 53(2): 65-72. DOI: 10.6054/j.jscnun.2021028

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The Preparation of Compound Hydrolases for Detergent from Excess Activated Sludge

1.
School of Life Sciences//Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, South China Normal University, Guangzhou 510631, China
2.
Shenzhen Shengshi Group, Shenzhen 518112, China
3.
Guangzhou Keteng Biological Medicine Technology Co., Ltd., Guangzhou 510425, China

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Received Date: May 10, 2020
Available Online: April 28, 2021

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Abstract

Abstract

The preparation of compound hydrolases for detergent from excess activated sludge was studied. The sludge was mixed with 2.0-fold water containing 1% Triton X-100 and stirred for 60 min. The rate of protease extraction was 71.7%. After membrane filtration and concentration, the enzyme extract was precipitated with 70% ethanol and freeze-dried to powder. Enzyme powder of 1.7 g was obtained from 1 kg sludge and the powder had an enzyme activity of 4.216, 3.714, 11.915, 3.060 and 1.291 U/mg for collagenase, alkaline protease, lipase, amylase and cellulose, respectively. The stability and the shelf life of the enzymes was greatly improved with the addition of an optimized complex stabilizer which consisted of 4% sucrose, 4% mannitol, 30 mmol/L glycine and 10 mmol/L magnesium sulfate. The commercial enzyme granules for detergent were prepared according to the formula of 4% composite enzyme powder, 10% diatomite, 10% soluble starch, 6% composite stabilizer and 70% sodium sulfate decahydrate. The main types of enzymes and the activities of enzyme granule preparation were similar to those of the commercial detergent enzyme preparation on the market. This study provides a new way for the utilization of activated sludge.
- excess activated sludge,
- complex hydrolases,
- isolation,
- complex enzyme stabilizer,
- granular detergent enzyme preparation

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[1]	赵顺顺. 剩余污泥蛋白质提取及其作为动物饲料添加剂的可行性研究[D]. 青岛: 中国海洋大学, 2007. ZHAO S S. Extraction of protein from waste sludge and its feasibility as an animal feed additive[D]. Qingdao: Ocean University of China, 2007.
[2]	蔡家璇, 张盼月, 张光明. 城市污泥中蛋白质资源化的研究进展[J]. 环境工程, 2019, 37(3): 17-22. https://www.cnki.com.cn/Article/CJFDTOTAL-HJGC201903004.htm CAI J X, ZHANG P Y, ZHANG G M. Research progress on protein resource utilization in municipal sludge[J]. Environmental Engineering, 2019, 37(3): 17-22. https://www.cnki.com.cn/Article/CJFDTOTAL-HJGC201903004.htm
[3]	TEOH S K, LI L Y. Feasibility of alternative sewage sludge treatment methods from a lifecycle assessment (LCA) perspective[J]. Journal of Cleaner Production, 2020, 247: 1-20. http://www.sciencedirect.com/science/article/pii/S0959652619343653
[4]	VINAY K T, SHANG L L. Sludge: a waste or renewable source for energy and resources recovery?[J]. Renewable and Sustainable Energy Reviews, 2013, 25: 708-728. doi: 10.1016/j.rser.2013.05.029
[5]	BLANCHARD M, TEIL M J, OLLIVON D, et al. Polycyclic aromatic hydrocarbons and polychlorobiphenyls in wastewaters and sewage sludges from the Paris area (France)[J]. Environmental Research, 2004, 95(2): 184-197. doi: 10.1016/j.envres.2003.07.003
[6]	AL-GHEETHI A, NOMAN E, BALA J D, et al. Removal of pathogenic bacteria from sewagetreated effluent and biosolids for agricultural purposes[J]. Applied Water Science, 2018, 8(2): 74: 92-97.
[7]	LI R D, TENG W C, LI Y L, et al. Potential recovery of phosphorus during the fluidized bed incineration of sewage sludge[J]. Journal of Cleaner Production, 2017, 140(2): 964-970. http://www.sciencedirect.com/science/article/pii/S0959652616308642
[8]	NI H, FAN X M, GUO H N, et al. Comprehensive utilization of activated sludge for preparation of hydrolytic enzymes, polyhydroxyalkanoates and water-retaining organic fertilizer[J]. Preparative Biochemistry & Biotechnology, 2017, 47(6): 611-618. http://www.ncbi.nlm.nih.gov/pubmed/28165864
[9]	赵向阳. 响应面法酶解剩余活性污泥中微生物蛋白质研究[J]. 华南师范大学学报(自然科学版), 2014(46): 85-91. doi: 10.6054/j.jxcnun.2014.06.126 ZHAO X Y. Enzymatic hydrolysis of microbial protein from excess activated sludge by Response Surface Methodology[J]. Journal of South China Normal University (Natural Science Edition), 2014(46): 85-91. doi: 10.6054/j.jxcnun.2014.06.126
[10]	KARN S K, KUMAR A. Hydrolytic enzyme protease in sludge: recovery and its application[J]. Biotechnology and Bioprocess Engineering, 2015, 20(4): 652-661. doi: 10.1007/s12257-015-0161-6
[11]	GARCIA M, URREA J L, COLLADO S, et al. Protein recovery from solubilized sludge by hydrothermal treatments[J]. Waste Management, 2017, 67: 278-287. doi: 10.1016/j.wasman.2017.05.051
[12]	李恺, 叶志平, 李焕文, 等. 表面活性剂CTAC对活性污泥的脱水性能及其机理研究[J]. 华南师范大学学报(自然科学版), 2010(2): 76-81. http://journal-n.scnu.edu.cn/article/id/206 LI K, YE Z P, LI H W et al. Study on dewatering performance and mechanism of surfactant CTAC for activated sludge[J]. Journal of South China Normal University (Natural Science Edition), 2010(2): 76-81. http://journal-n.scnu.edu.cn/article/id/206
[13]	DANIEL J A, FUNSO E O. Experimental investigations on the effects of carbon and nitrogen sources on concomitant amylase and polygalacturonase production byTrichoderma viride, BITRS-1001 in submerged fermentation[J]. Biotechnology Research International, 2012(2012): 904763/1-8. http://pubmedcentralcanada.ca/pmcc/articles/PMC3418636/
[14]	王小骊, 柳爱春, 邹晓庭, 等. 蛋白酶制剂蛋白酶活力的测定福林法: GB/T 28715-2012[S]. 北京: 中国标准出版社, 2012-09-03.
[15]	张渊舒, 罗琴, 攸德伟. 植酸酶颗粒剂生产工艺的研究[J]. 江西农业学报, 2011(5): 128-129, 139. https://www.cnki.com.cn/Article/CJFDTOTAL-JXNY201105041.htm ZHANG Y S, LUO Q, YOU D W. Study on the production process of phytase granules[J]. JiangXi Journal of Agricultural Sciences, 2011(5): 128-129, 139. https://www.cnki.com.cn/Article/CJFDTOTAL-JXNY201105041.htm
[16]	NABARLATZ D, VONDRYSOVA J, JENICEK P, et al. Hydrolytic enzymes in activated sludge: extraction of protease and lipase by stirring and ultrasonication[J]. Ultrasonics Sonochemistry, 2010, 17(5): 923-931. doi: 10.1016/j.ultsonch.2010.02.006
[17]	KARN S K, KUMAR P, PAN X. Extraction of lipase ansprotease and characterization of activated sludge from pulp and paper industry[J]. Preparative Biochemistry & Biotechnology, 2013, 43(2): 152-162. http://europepmc.org/abstract/MED/23302103
[18]	NABARLATZ D, FRANK S, FONT J, et al. Extraction and purification of hydrolytic enzymes from activated sludge[J]. Resources, Conservation and Recycling, 2012, 59: 9-13. doi: 10.1016/j.resconrec.2011.06.017
[19]	CAPITO F, SKUDAS R, BERND S. Polyelectrolyte-protein interaction at low ionic strength: required chain flexibility depending on protein average charge[J]. Colloid & Polymer Science, 2013, 291(7): 1759-1769. doi: 10.1007/s00396-013-2911-3
[20]	SEKIGUCHI S, HASHIDA Y, YASUKAWA K, et al. Stabilization of bovine intestine alkaline phosphatase bysugars[J]. Bioscience Biotechnology and Biochemistry, 2012, 76(1): 95-100. doi: 10.1271/bbb.110553
[21]	HAN Q H, SUN X H, XU M X, et al. 3-Deazaadenosine, a stabilizer of whole-blood homocysteine content, does not interfere with the single-enzyme homocysteine assay while totally inhibiting the enzyme conversion homocysteine immunoassay[J]. Clinical Chemistry, 2020, 9: 8-9.
[22]	任立均, 刘龙, 刘松, 等. 谷氨酰胺转氨酶热稳定剂优化[J]. 食品与发酵工业, 2018, 44(1): 1-6. https://www.cnki.com.cn/Article/CJFDTOTAL-SPFX201801001.htm REN L J, LIU L, LIU S, et al. Optimization of heat stabilizer of transglutaminase[J]. Food and Fermentation Industry, 2018, 44(1): 1-6. https://www.cnki.com.cn/Article/CJFDTOTAL-SPFX201801001.htm
[23]	ZHANG J, LI M, ZHANG Y. Enhancing the thermostability of recombinant cyclodextrin glucanotransferase via optimized stabilizer[J]. Process Biochemistry, 2018, 67: 64-70. doi: 10.1016/j.procbio.2018.02.006
[24]	PAZHANG M, MEHRNEJAD F, PAZHANG Y, et al. Effect of sorbitol and glycerol on the stability of trypsinand difference between their stabilization effects in the various solvents[J]. Biotechnology and Applied Biochemistry, 2016, 63(2): 206-213. doi: 10.1002/bab.1366
[25]	KUMAR V, CHARI R, SHARMA V K, et al. Modulation of the thermodynamic stability of proteins by polyols: significance of polyol hydrophobicity and impact on the chemical potential of water[J]. International Journal of Pharmaceutics, 2011, 413(1/2): 19-28. http://www.ncbi.nlm.nih.gov/pubmed/21515346
[26]	张京良, 姜言晖, 朱常亮, 等. 复合保护剂对透明质酸酶的稳定性研究[J]. 食品工业科技, 2018(13): 25-29. https://www.cnki.com.cn/Article/CJFDTOTAL-SPKJ201813005.htm ZHANG J L, JIANG Y H, ZHU C L, et al. Study on the stability of compound protective agent to hyaluronidase[J]. Food Industry Technology, 2018(13): 25-29. https://www.cnki.com.cn/Article/CJFDTOTAL-SPKJ201813005.htm

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