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LI Jinling, YANG Yuanyuan, LIU Sisi, ZHAO Jianliang, YING Guangguo, CHEN Changer. Determining Five Fluoroquinolone Antibiotics in Water Samples with the UPLC-QTOF Method[J]. Journal of South China Normal University (Natural Science Edition), 2021, 53(2): 44-50. DOI: 10.6054/j.jscnun.2021025
Citation: LI Jinling, YANG Yuanyuan, LIU Sisi, ZHAO Jianliang, YING Guangguo, CHEN Changer. Determining Five Fluoroquinolone Antibiotics in Water Samples with the UPLC-QTOF Method[J]. Journal of South China Normal University (Natural Science Edition), 2021, 53(2): 44-50. DOI: 10.6054/j.jscnun.2021025
shu

Determining Five Fluoroquinolone Antibiotics in Water Samples with the UPLC-QTOF Method

  • MOE Key Laboratory of Environmental Theoretical Chemistry//Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety//Environmental Research Institute, School of Environment, South China Normal University, Guangzhou 510006, China

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  • Received Date: October 20, 2020
  • Available Online: April 28, 2021
    Graphical Abstract
    • Abstract
  • A quantitative method was developed for the simultaneous determination of five fluoroquinolones (FQs), including ciprofloxacin, enrofloxacin, norfloxacin, ofloxacin and pefloxacin, using ultra performance liquid chromatography-quadruple time of flight mass spectrometry (UPLC-QTOF). Samples were separated on Waters ACUITY UPLC BEH C18 column with gradient elution programs, using methanol and water containing 0.1% formic acid as the mobile phases, and analyzed in electrospray positive ion source, using three acquisition modes (MS, MSE and MRM). Five FQs were quantified with an internal standard method. Limits of detection (LOD) and limits of quantification (LOQ) of the 5 FQs on the UPLC-QTOF were 0.04~0.22 μg/L and 0.17~0.90 μg/L, respectively. Linear ranges of the 5 FQs were 1~100 μg/L with good correlation coefficients (R2≥0.99). This method was compared with the UPLC-tandem mass spectrometry (UPLC-TQMS) method and showed comparable performance. UPLC-QTOF is fast, sensitive, and suitable for the simultaneous quantification of the 5 FQs in environmental water samples.
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