The Effect of Electrical Stimulation on Weightlessness-Caused Waste Skeletal Muscular Atrophy

SHI Haiwang; DUAN Rui; LIU Chengyi

doi:10.6054/j.jscnun.2020095

Journal of South China Normal University (Natural Science Edition) > 2020 > 52(6): 57-66. > DOI: 10.6054/j.jscnun.2020095

SHI Haiwang, DUAN Rui, LIU Chengyi. The Effect of Electrical Stimulation on Weightlessness-Caused Waste Skeletal Muscular Atrophy[J]. Journal of South China Normal University (Natural Science Edition), 2020, 52(6): 57-66. DOI: 10.6054/j.jscnun.2020095

Citation:

PDF (2296 KB)

The Effect of Electrical Stimulation on Weightlessness-Caused Waste Skeletal Muscular Atrophy

School of Physical Education and Sport Science, South China Normal University, Guangzhou 510006, China

More Information

Received Date: December 16, 2019
Available Online: January 04, 2021

Graphical Abstract

Abstract

Abstract

Skeletal muscular atrophy refers to the decrease of skeletal muscle mass caused by denervation, injury, joint fixation, bed rest, septicemia, cancer, aging and microgravity. Skeletal muscle electrical stimulation (MES) was used to simulate different forms of exercise to explore the effect of delaying skeletal muscular atrophy in Sprague-Dawley (SD) rats caused by tail suspension (TS). The combination training mode was introduced to further explore whether the effect of maintaining habitual exercise mode on delaying skeletal muscular atrophy is better than that of non-habitual exercise mode. The sigma algorithm and traditional statistical methods were used to analyze the experimental results. The results showed that after 6 weeks of high frequency electrical stimulation (HFES) intervention, skeletal muscle mass was significantly improved, in which mass of left soleus (m_LS) and left tibialis (m_LT) increased by 21% (P < 0.01) and 26% (P < 0.01) respectively. The protein activation of Akt and p70s6k increased significantly. After 6 weeks of low frequency electrical stimulation (LFES) intervention, the mass of soleus and tibia-lis tended to increase, but there was no significant difference compared with group Z. After 4 weeks of TS treatment, the mass of soleus decreased by 45% (P < 0.01) compared with group Z, indicating that the model was successfully constructed. The degree of decline was significantly improved after normal gravity and microgravity intervention, and the mass of soleus increased by 49% (P < 0.01) compared with group T after normal gravity intervention. The intervention results of combination group showed that compared with group T, the mass of soleus and tibialis in combination group (group HHT) increased by 55% (P < 0.01) and 37% (P < 0.01) respectively, the mass of left gastrocnemius (m_LG) increased by 25% (P < 0.01), and the overall intervention effect was better than that of other groups. In conclusion, the effect of high-frequency electrical stimulation on increasing skeletal muscle mass is better than that of low-frequency electrical stimulation, and the effect of normal gravity intervention is better than that of microgravity intervention, and the habitual exercise intervention mode is better than that of the non-habitual exercise in delaying soleus muscular atrophy caused by weightlessness.
- skeletal muscular atrophy,
- electrical stimulation,
- tail suspension,
- habitual

FullText(HTML)

References (28)

References

[1]	MCKINNELL I W, RUDNICKI M A. Molecular mechanisms of muscle atrophy[J]. Cell, 2004, 119(7):907-910. http://europepmc.org/abstract/med/15620349
[2]	BOONYAROM O, INUI K. Atrophy and hypertrophy of skeletal muscles:structural and functional aspects[J]. Acta Physiologica (Oxf), 2006, 188(2):77-89. http://europepmc.org/abstract/MED/16948795
[3]	BONALDO P, SANDRI M. Cellular and molecular mechanisms of muscle atrophy[J]. Disease Model Mechanisms, 2013, 6(1):25-39. http://pubmedcentralcanada.ca/pmcc/articles/PMC3529336/
[4]	LATRES E, AMINI A R, AMINI A A, et al. Insulin-like growth factor-1 (IGF-1) inversely regulates atrophy-induced genes via the phosphatidylinositol 3-kinase/Akt/mammalian target of rapamycin (PI3K/Akt/mTOR) pathway[J]. Journal of Biological Chemistry, 2005, 280(4):2737-2744. http://hmg.oxfordjournals.org/cgi/ijlink?linkType=ABST&journalCode=jbc&resid=280/4/2737
[5]	SCHIAFFINO S, MAMMUCARI C. Regulation of skeletal muscle growth by the IGF1-Akt/PKB pathway:insights from genetic models[J]. Skelet Muscle, 2011, 1(1):4-17. doi: 10.1186/2044-5040-1-4
[6]	RATTI F, RAMOND F, MONCOLLIN V, et al. Histone deacetylase 6 is a FoxO transcription factor-dependent effector in skeletal muscle atrophy[J]. Journal of Biological Chemistry, 2015, 290(7):4215-4224. http://www.ncbi.nlm.nih.gov/pubmed/25516595
[7]	BODINE S C, LATRES E, BAUMHUETER S, et al. Identification of ubiquitin ligases required for skeletal muscle atrophy[J]. Science, 2001, 294(5547):1704-1708. http://www.ncbi.nlm.nih.gov/pubmed/11679633/
[8]	SANDRI M. FOXOphagy path to inducing stress resistance and cell survival[J]. Nature Cell Biology, 2012, 14(8):786-788. http://www.nature.com/articles/ncb2550
[9]	ZHAO J, BRAULT J J, SCHILD A, et al. FoxO3 coordinately activates protein degradation by the autophagic/lysosomal and proteasomal pathways in atrophying muscle cells[J]. Cell Metablism, 2007, 6(6):472-483. http://europepmc.org/abstract/med/18054316
[10]	MCPHERRON A C, LAWLER A M, LEE S J. Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member[J]. Nature, 1997, 387(6628):83-90. http://www.tandfonline.com/servlet/linkout?suffix=CIT0031&dbid=8&doi=10.3109%2F08977194.2011.599324&key=9139826
[11]	EKEN T. Spontaneous electromyographic activity in adult rat soleus muscle[J]. Journa of Neurophysiology, 1998, 80(1):365-376. http://www.ncbi.nlm.nih.gov/pubmed/9658057
[12]	PETTERSON S, SNYDER-MACKLER L. The use of neuromuscular electrical stimulation to improve activation deficits in a patient with chronic quadriceps strength impairments following total knee arthroplasty[J]. Journal of Orthopaedic and Sports Physical Therapy, 2006, 36(9):678-685. http://labs.europepmc.org/abstract/MED/17017273
[13]	DUPONT SALTER A C, RICHMOND F J, LOEB G E. Prevention of muscle disuse atrophy by low-frequency electrical stimulation in rats[J]. IEEE Transactions on Neural Systems and Rehabilitation Engineering, 2003, 11(3):218-226. http://europepmc.org/abstract/MED/14518784
[14]	NAKAGAWA K, TAMAKI H, HAYAO K, et al. Electrical stimulation of denervated rat skeletal muscle retards capillary and muscle loss in early stages of disuse atrophy[J]. BioMed Research International, 2017, 56(9):521-527. http://europepmc.org/abstract/MED/28497057
[15]	TANAKA M, HIRAYAMA Y, FUJITA N, et al. Electrical stimulation using sine waveform prevents unloading-induced muscle atrophy in the deep calf muscles of rat[J]. Acta Histochemica, 2014, 116(7):1192-1198. http://www.ncbi.nlm.nih.gov/pubmed/25028130
[16]	陈杰, 马进, 丁兆平.一种模拟长期失重影响的大鼠尾部悬吊模型[J].空间科学学报, 1993, 13(2):159-162. http://www.cnki.com.cn/Article/CJFDTotal-KJKB199302011.htm CHEN J, MA J, DING Z P, et al. A modified tail-suspension model for simulating long-term weightlessness[J]. Chinese Journal of Space Science, 1993, 13(2):159-162. http://www.cnki.com.cn/Article/CJFDTotal-KJKB199302011.htm
[17]	LIU T C, TANG X M, DUAN R, et al. The mitochondrial Na(+)/Ca(2+) exchanger is necessary but not sufficient for Ca(2+) homeostasis and viability[J]. Advances in Experimental Medicine and Biology, 2018, 1072:281-285. http://www.ncbi.nlm.nih.gov/pubmed/30178359
[18]	LIU T C, LIU G, HU S J, et al. Quantitative biology of exercise-induced signal transduction pathways[J]. Advances in Experimental Medicine and Biology, 2017, 977:419-424. http://europepmc.org/abstract/MED/28685473
[19]	TALEB N N. The black swan :the impact of the highly improbable[M]. Revised Edition. London:Allen Lane, 2011.
[20]	FECHNER G T, ADLER H E, HOWES D H, et al. Elements of psychophysics[M]. New York:Holt, Rinehart and Winston, 1966.
[21]	刘承宜, 胡少娟, 李晓云.定量差异及其在体育科学中的应用[J].体育学刊, 2016, 23(1):11-17. LIU C Y, HU S J, LI X Y. Quantitative difference and its application in sports science[J]. Journal of Physical Education, 2016, 23(1):11-17.
[22]	SUN S S, HU C L, PAN J H, et al. Trait mindfulness is associated with the self-similarity of heart rate variability[J]. Frontiers in Psychology, 2019(10):314/1-11. http://www.ncbi.nlm.nih.gov/pubmed/30873070
[23]	刘承宜, 朱玲, 李方晖.自相似常数和定量差异及其在体育科学中的应用[J].体育学刊, 2017, 151(6):78-84. http://d.wanfangdata.com.cn/periodical/tyxk201706015 LIU C Y, ZHU L, LI F H. Self-similar constant and quantitative difference as well as their applications in sports science[J]. Journal of Physical Education, 2017, 151(6):78-84. http://d.wanfangdata.com.cn/periodical/tyxk201706015
[24]	刘承宜, 杨罗丹, 吴冲云.慢性膝关节疼痛针刺治疗的表型组学再分析[J].中国科技论文在线精品论文, 2017, 10(15):1780-1785. LIU C Y, YANG L D, WU C Y, et al. Phenomics reanalysis on therapeutic effects of acupuncture on chronic knee pain[J]. Highlights of Sciencepaper Online, 2017, 10(15):1780-1785.
[25]	ZHANG P, CHEN X, FAN M. Signaling mechanisms involved in disuse muscle atrophy[J]. Medical Hypotheses, 2007, 69(2):310-321. http://europepmc.org/abstract/MED/17376604
[26]	高久翔, 张宇纯, 于亮.电针对小鼠下肢肌萎缩干预效果的研究[J].中国康复医学杂志, 2018(3):280-285. http://www.cqvip.com/QK/90850X/201803/674849038.html GAO J X, ZHANG Y C, YU L. Electric acupuncture intervention on the effects of disuse muscular atrophy muscle fiber types of mice[J]. Chinese Journal of Rehabilitation Medicine, 2018(3):280-285. http://www.cqvip.com/QK/90850X/201803/674849038.html
[27]	黄惠芝.红景天苷对COPD大鼠骨骼肌的保护作用及与PI3K/Akt/mTOR的关系[D].衡阳: 南华大学, 2017. HUANG H Z. The effect of salidrosidein protecting skeletal muscle in rats with chronic obstructive pulmonary disease and the relationship with PI3K/AKT/mTOR[D].Hengyang: University of South China, 2017.
[28]	任晋华, 梁炳生, 彭春辉. FoXO3a通路调控失神经肌萎缩的作用机制及银杏叶提取物的治疗作用[J].中国医疗前沿, 2012, 7(3):42-44. http://d.wanfangdata.com.cn/Thesis/Y2126713 REN J H, LIANG B S, PENG C H. The modulating mechanism of FoXO3a in denervated skeletal muscle atrophy in rats and the treating effects of the extract of Ginkgo Biloba(EGB761)[J]. National Medical Frontiers of China, 2012, 7(3):42-44. http://d.wanfangdata.com.cn/Thesis/Y2126713

[1]	MENG Zhan, BAI Heng, ZHAO Lei. Phase Equilibrium of CO₂-Hydrocarbon Mixture in Confined Space with Extended PT Equation of State[J]. Journal of South China Normal University (Natural Science Edition), 2025, 57(1): 43-50. DOI: 10.6054/j.jscnun.2025005
[2]	HE Guannan, HUANG Bo. Preparation of ZnO Light Trapping Materials and their Performance in Solar Cells[J]. Journal of South China Normal University (Natural Science Edition), 2019, 51(4): 1-6. DOI: 10.6054/j.jscnun.2019056
[3]	Degradation of Rhodamine B by Carbon Nitride Activated Sodium Persulfate under Visible Light Irradiation[J]. Journal of South China Normal University (Natural Science Edition), 2017, 49(3): 44-48.
[4]	Research Progress on Light Extraction Technology of LED[J]. Journal of South China Normal University (Natural Science Edition), 2016, 48(5): 1-7. DOI: 10.6054/j.jscnun.2016.08.001
[5]	Sheng Qian, Xue Jianming*. The Research Progress of Room Temperature Ionic Liquids in Confined Geometries[J]. Journal of South China Normal University (Natural Science Edition), 2015, 47(2): 27-31. DOI: 10.6054/j.jscnun.2014.12.014
[6]	Theoretical Study of Light (Electrostatics) Driven Molecular Switching Processes[J]. Journal of South China Normal University (Natural Science Edition), 2014, 46(6): 139-139.
[7]	Microemulsion Synthesis Of Nanosized Bismuth Oxyiodide-Titanium Dioxide Composite Particles And Photodegradation Of Bisphenol A Under Visible Light Irradiation[J]. Journal of South China Normal University (Natural Science Edition), 2013, 45(1). DOI: 10.6054/j.jscnun.2012.12.013
[8]	Kan Xue-min. photon mass and dispersion of the velocity of light in vacuum[J]. Journal of South China Normal University (Natural Science Edition), 2012, 44(3). DOI: 10.6054/j.jscnun.2012.06.016
[9]	THE FORMATION OF CHARGE-TRANSFER-COMPLEX OF ORGANICS/TIO2 AND THEIR EFFECTS ON PHOTOCATALYTIC REDUCTION OF CR6+ UNDER VISIBLE LIGHT IRRADIATION[J]. Journal of South China Normal University (Natural Science Edition), 2012, 44(2).
[10]	EXPERIMENTAL RESEARCH OF PHASE-SHIFTING DIGITAL HOLOGRAPHY BASED ON SPATIAL LIGHT MODULATOR[J]. Journal of South China Normal University (Natural Science Edition), 2009, 1(2): 44-47 .

Cited By

Cited by

Periodical cited type(2)

1.	管钰晴，唐冬梅，傅云霞，孙佳媛，韩志国，张波，孔明，曹程明，雷李华. 穆勒椭偏标定方法中LM算法研究. 红外与激光工程. 2020(08): 168-176 .
2.	汪娟，冀丽娜，白芸，黄佐华. 单波长椭偏法测量各向异性晶体光学参数的研究. 激光与光电子学进展. 2020(15): 224-232 .

Other cited types(2)

Get Citation

PDF

XML

Article views PDF downloads Cited by(4)

Turn off MathJax

Article Contents

Abstract

References

The Effect of Electrical Stimulation on Weightlessness-Caused Waste Skeletal Muscular Atrophy