WU Qianqian, HU Minlun, ZHONG Yun, YAN Huaxue, JIANG Bo, WU Bo, ZHONG Guangyan, GAO Feng. Construction of Mi Gene Over-Expression Vector and 16D10 Gene RNAi Vector and Their Transformation[J]. Journal of South China Normal University (Natural Science Edition), 2018, 50(4): 68-73. DOI: 10.6054/j.jscnun.2018079
Citation:
WU Qianqian, HU Minlun, ZHONG Yun, YAN Huaxue, JIANG Bo, WU Bo, ZHONG Guangyan, GAO Feng. Construction of Mi Gene Over-Expression Vector and 16D10 Gene RNAi Vector and Their Transformation[J]. Journal of South China Normal University (Natural Science Edition), 2018, 50(4): 68-73. DOI: 10.6054/j.jscnun.2018079
WU Qianqian, HU Minlun, ZHONG Yun, YAN Huaxue, JIANG Bo, WU Bo, ZHONG Guangyan, GAO Feng. Construction of Mi Gene Over-Expression Vector and 16D10 Gene RNAi Vector and Their Transformation[J]. Journal of South China Normal University (Natural Science Edition), 2018, 50(4): 68-73. DOI: 10.6054/j.jscnun.2018079
Citation:
WU Qianqian, HU Minlun, ZHONG Yun, YAN Huaxue, JIANG Bo, WU Bo, ZHONG Guangyan, GAO Feng. Construction of Mi Gene Over-Expression Vector and 16D10 Gene RNAi Vector and Their Transformation[J]. Journal of South China Normal University (Natural Science Edition), 2018, 50(4): 68-73. DOI: 10.6054/j.jscnun.2018079
(1. Guangdong Provincial Key Laboratory of Biotechnology for Plant Development∥School of Life Sciences,South China Normal University,Guangzhou 510631,China;
2.
2. Institute of Fruit Tree Research,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;
3.
3. Key Laboratory of South Subtropical Fruit Biology and Genetice Resource Utilization∥Guangdong Province Key Laboratory of Tropical and Subtropical Fruit Tree Research,Guangzhou 510640,China)
Mi gene was cloned from tomato with PCR amplification and 16D10 gene was cloned from root knot nematode in Citus reticulate with reverse-transcription PCR (RT-PCR) amplification. The Mi gene over-expression vector (named pB-Mi) and 16D10 gene RNAi vector (named pB-16DR) were constructed with DNA molecular recombination technology. Putative transgenic plantlets, which were confirmed by PCR analysis, were obtained through genetic transformation mediated by Agrobacterium tumefaciens. The results can provide material and technical basis for exploring the effect of the over-expression of Mi gene and RNA interference of 16D10 gene on the resistance of Citrus root knot nematode disease.
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