The Preparation of High-purity Crystalline Glucose and Soluble Dietary Fiber through Enzymolysis of Sweet Potato Residue

SHI Shuaishuai; XI Yi; NI He; LÜ Zhencheng; CHEN Zhaogui; LI Haihang

doi:10.6054/j.jscnun.2021059

Journal of South China Normal University (Natural Science Edition) > 2021 > 53(4): 61-67. > DOI: 10.6054/j.jscnun.2021059

SHI Shuaishuai, XI Yi, NI He, LÜ Zhencheng, CHEN Zhaogui, LI Haihang. The Preparation of High-purity Crystalline Glucose and Soluble Dietary Fiber through Enzymolysis of Sweet Potato Residue[J]. Journal of South China Normal University (Natural Science Edition), 2021, 53(4): 61-67. DOI: 10.6054/j.jscnun.2021059

Citation:

PDF (1099 KB)

The Preparation of High-purity Crystalline Glucose and Soluble Dietary Fiber through Enzymolysis of Sweet Potato Residue

1.
School of Life Sciences//Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, South China Normal University, Guangzhou 510631, China
2.
School of Life Sciences, Huizhou University, Huizhou 516007, China

More Information

Received Date: April 15, 2020
Available Online: September 02, 2021

Graphical Abstract

Abstract

Abstract

A practical method for preparing high-purity crystalline glucose through enzymolysis of sweet potato residue was developed. Freshly prepared sweet potato residue, mixed with water of equal volume, was first treated with 20 U/g thermostable α-amylase at 90 ℃ for 60 min and then with 300 U/g glucoamylase at 60 ℃ for 6 h. The hydrolyzed glucose solution was treated with 1% macroporous resin to remove the impurities, vacuum-concentrated to glucose content of 70%, and crystallized at 4 ℃ for 48 h. After being washed twice with 95% ethanol and vacuum-dried, crystallized glucose with purity higher than 99% was obtained. After hydrolysis, the remaining sweet potato residue was mixed with 150 U/g cellulase and reacted at pH 5.5 and 40 ℃ for 180 min. The yield of soluble dietary fiber increased from 2% to 20%. This research provides a simple and practical method for efficient utilization of sweet potato residue and reduction of resource waste and ecological pollution.
- sweet potato residue,
- enzymolysis,
- high-purity crystalline glucose,
- soluble dietary fiber

FullText(HTML)

References (19)

References

[1]	乔汉桢, 刘佳琪, 许雯雯, 等. 甘薯渣膳食纤维的制备及改性工艺研究进展[J]. 饲料研究, 2019, 42(7): 89-94. https://www.cnki.com.cn/Article/CJFDTOTAL-SLYJ201907025.htm QIAO H Z, LIU J Q, XU W W, et al. Preparation and modification of dietary fiber from sweet potato residues[J]. Feed Research, 2019, 42(7): 89-94. https://www.cnki.com.cn/Article/CJFDTOTAL-SLYJ201907025.htm
[2]	ABEGUNDE O K, MU T H, CHEN J W, et al. Physicochemical characterization of sweet potato starches popularly used in Chinese starch industry[J]. Food Hydrocolloids, 2013, 33(2): 169-177. doi: 10.1016/j.foodhyd.2013.03.005
[3]	李青嵘, 罗嘉玲, 程实, 等. 甘薯淀粉加工废水中生化成分回收的新方法研究[J]. 华南师范大学学报(自然科学版), 2018, 50(2): 57-64. http://journal-n.scnu.edu.cn/article/id/4329 LI Q R, LUO J L, CHENG S, et al. New method for the comprehensive recovery of biochemical components from sweet potato wastewater in strach production[J]. Journal of South China Normal University (Natural Science Edition), 2018, 50(2): 57-64. http://journal-n.scnu.edu.cn/article/id/4329
[4]	LI Q R, LUO J L, ZHOU Z H, et al. Simplified recovery of enzymes and nutrients in sweet potato wastewater and preparing health black tea and theaflavins with scrap tea[J]. Food Chemistry, 2018, 245(15): 854-862. http://www.sciencedirect.com/science/article/pii/S0308814617319143
[5]	CHENG S, ZHANG Y F, ZENG Z Q, et al. Screening, separating, and completely recovering polyphenol oxidases and other biochemicals from sweet potato wastewater in starch production[J]. Applied Microbiology & Biotechnology, 2015, 99(4): 1745-1753. http://www.ncbi.nlm.nih.gov/pubmed/25190667
[6]	AHMED M, AKTER M S, EUN J B. Peeling, drying temperatures, and sulphite-treatment affect physicochemical properties and nutritional quality of sweet potato flour[J]. Food Chemistry, 2010, 121(1): 112-118. doi: 10.1016/j.foodchem.2009.12.015
[7]	汤月敏, 代养勇, 高歌, 等. 我国甘薯产业现状及其发展趋势[J]. 中国食物与营养, 2010(8): 23-26. doi: 10.3969/j.issn.1006-9577.2010.08.006 TANG Y M, DAI Y Y, GAO G, et al. Status of sweet potato industry in China and its dvelopment trend[J]. Food and Nutrition in China, 2010(8): 23-26. doi: 10.3969/j.issn.1006-9577.2010.08.006
[8]	江阳, 孙成均. 甘薯的营养成分及其保健功效研究进展[J]. 中国农业科技导报, 2010, 12(4): 56-61. doi: 10.3969/j.issn.1008-0864.2010.04.11 JIANG Y, SUN C J. Advances in studies on nutritious components of sweet potato and their health-promoting functions[J]. Journal of Agricultural Science and Technology, 2010, 12(4): 56-61. doi: 10.3969/j.issn.1008-0864.2010.04.11
[9]	徐梦瑶, 赵祥颖, 张立鹤, 等. 甘薯的营养价值及保健作用[J]. 中国果菜, 2017, 37(5): 17-21, 47. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGGP201705006.htm XU M Y, ZHAO X Y, ZHANG L H, et al. The nutritional value and health care function of sweet potato[j]. China Fruit Vegetable, 2017, 37(5): 17-21, 47. https://www.cnki.com.cn/Article/CJFDTOTAL-ZGGP201705006.htm
[10]	全桂静, 宋文婷. 甘薯多糖提取条件及抗氧化性的研究[J]. 沈阳化工大学学报, 2014, 28(4): 325-328, 342. doi: 10.3969/j.issn.2095-2198.2014.04.009 QUANG G J, SONG W T. Extraction of polysaccharide from sweet potato and its antioxidation properties[J]. Journal of Shenyang University of Chemical Technology, 2014, 28(4): 325-328, 342. doi: 10.3969/j.issn.2095-2198.2014.04.009
[11]	KIM O K, YOO S A, NAM D E, et al. Immunomodulatory effects of curcuma longa L. extract in LP-BM5 murine leukemia viruses-induced murine acquired immune deficiency syndrome[J]. Journal of the Korean Society of Food Science and Nutrition, 2014, 43(9): 1317-1324. doi: 10.3746/jkfn.2014.43.9.1317
[12]	HWANG Y P, CHOI J H, CHOI J M, et al. Protective mechanisms of anthocyanins from purple sweet potato against tert-butyl hydroperoxide-induced hepatotoxicity[J]. Food and Chemical Toxicology: An International Journal Published for the British Industrial Biological Research Association, 2011, 49(9): 2081-2089. doi: 10.1016/j.fct.2011.05.021
[13]	邓奇风, 高凤仙. 甘薯渣的开发与应用[J]. 饲料博览, 2015(9): 47-49. doi: 10.3969/j.issn.1001-0084.2015.09.013 DENG Q F, GAO F X. Development and application of sweet potato residue[J]. Feed Review, 2015(9): 47-49. doi: 10.3969/j.issn.1001-0084.2015.09.013
[14]	刘惠知, 王升平, 周映华, 等. 红薯渣及其利用[J]. 饲料博览, 2013(7): 41-43. doi: 10.3969/j.issn.1001-0084.2013.07.011 LIU H Z, WANG S P, ZHOU Y H, et al. Sweet potato residue and its use[J]. Feed Review, 2013(7): 41-43. doi: 10.3969/j.issn.1001-0084.2013.07.011
[15]	母丽萍, 雷激, 李博, 等. 紫甘薯饮料制备工艺研究[J]. 食品科学, 2010, 31(20): 513-517. https://www.cnki.com.cn/Article/CJFDTOTAL-SPKX201020110.htm MU L P, LEI J, LI B, et al. Development of a purple sweet potato beverage[J]. Food Science, 2010, 31(20): 513-517. https://www.cnki.com.cn/Article/CJFDTOTAL-SPKX201020110.htm
[16]	孟悦, 王英哲, 田志刚, 等. 玉米粉制备葡萄糖的糖化工艺优化[J]. 食品研究与开发, 2019, 40(18): 97-101. doi: 10.12161/j.issn.1005-6521.2019.18.016 MENG Y, WANG Y Z, TIAN Z G, et al. Optimization of saccharification process for preparing glucose from corn flour[J]. Food Research and Development, 2019, 40(18): 97-101. doi: 10.12161/j.issn.1005-6521.2019.18.016
[17]	吴允山, 郭军, 严共鸿, 等. 甘薯渣酶法生产葡萄糖的技术研究[J]. 中国农业科技导报, 2014, 16(1): 157-162. https://www.cnki.com.cn/Article/CJFDTOTAL-NKDB201401034.htm WU Y S, GUO J, YAN Z H, et al. Studies on technique of producing glucose from sweet potato residue by enzymes[J]. Journal of Agricultural Science and Technology, 2014, 16(1): 157-162. https://www.cnki.com.cn/Article/CJFDTOTAL-NKDB201401034.htm
[18]	徐梦瑶. 甘薯渣的资源化利用[D]. 济南: 山东师范大学, 2017. XU M Y. Utilization of the sweet potato residue[D]. Jinan: Shandong Normal University, 2017.
[19]	孙健, 钮福祥, 岳瑞雪, 等. 超声波辅助酶法提取甘薯渣膳食纤维的研究[J]. 核农学报, 2014, 28(7): 1261-1266. https://www.cnki.com.cn/Article/CJFDTOTAL-HNXB201407019.htm SUN J, NIU F X, YUE R X, et al. Extraction of dietary fiber from sweetpotato residues by enzymatic hydrolysis method assisted by ultrasonic technology[J]. Journal of Nuclear Agricultural Sciences, 2014, 28(7): 1261-1266. https://www.cnki.com.cn/Article/CJFDTOTAL-HNXB201407019.htm

Cited By

Cited by

Periodical cited type(3)

1.	杨悦，包智明，张瑀琼，王靖波，刘禹全，吕新亮. 中药治疗寒湿痹阻型类风湿关节炎作用机制研究进展. 内蒙古中医药. 2024(07): 148-150 .
2.	车永琦，李文魁，张伟，程芳斌，王怀龙，王恒，李泽，刘壮. 复方杜仲汤联合托法替布及甲氨蝶呤治疗类风湿性关节炎的临床效果. 临床误诊误治. 2024(19): 74-78 .
3.	何诚明，李鑫，刘田. 桂枝芍药知母汤治疗类风湿关节炎的临床疗效. 深圳中西医结合杂志. 2024(21): 49-52 .

Other cited types(0)

Get Citation

PDF

XML

Article views (452) PDF downloads (31) Cited by(3)

Turn off MathJax

Article Contents

Abstract

References

The Preparation of High-purity Crystalline Glucose and Soluble Dietary Fiber through Enzymolysis of Sweet Potato Residue

Abstract

References

Cited by

Periodical cited type(3)

Other cited types(0)

Catalog

Export File

Citation

Format

Content