The Preparation of High-purity Crystalline Glucose and Soluble Dietary Fiber through Enzymolysis of Sweet Potato Residue

SHI Shuaishuai; XI Yi; NI He; LÜ Zhencheng; CHEN Zhaogui; LI Haihang

doi:10.6054/j.jscnun.2021059

Journal of South China Normal University (Natural Science Edition) > 2021 > 53(4): 61-67. > DOI: 10.6054/j.jscnun.2021059

SHI Shuaishuai, XI Yi, NI He, LÜ Zhencheng, CHEN Zhaogui, LI Haihang. The Preparation of High-purity Crystalline Glucose and Soluble Dietary Fiber through Enzymolysis of Sweet Potato Residue[J]. Journal of South China Normal University (Natural Science Edition), 2021, 53(4): 61-67. DOI: 10.6054/j.jscnun.2021059

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The Preparation of High-purity Crystalline Glucose and Soluble Dietary Fiber through Enzymolysis of Sweet Potato Residue

1.
School of Life Sciences//Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, South China Normal University, Guangzhou 510631, China
2.
School of Life Sciences, Huizhou University, Huizhou 516007, China

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Received Date: April 15, 2020
Available Online: September 02, 2021

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Abstract

Abstract

A practical method for preparing high-purity crystalline glucose through enzymolysis of sweet potato residue was developed. Freshly prepared sweet potato residue, mixed with water of equal volume, was first treated with 20 U/g thermostable α-amylase at 90 ℃ for 60 min and then with 300 U/g glucoamylase at 60 ℃ for 6 h. The hydrolyzed glucose solution was treated with 1% macroporous resin to remove the impurities, vacuum-concentrated to glucose content of 70%, and crystallized at 4 ℃ for 48 h. After being washed twice with 95% ethanol and vacuum-dried, crystallized glucose with purity higher than 99% was obtained. After hydrolysis, the remaining sweet potato residue was mixed with 150 U/g cellulase and reacted at pH 5.5 and 40 ℃ for 180 min. The yield of soluble dietary fiber increased from 2% to 20%. This research provides a simple and practical method for efficient utilization of sweet potato residue and reduction of resource waste and ecological pollution.
- sweet potato residue,
- enzymolysis,
- high-purity crystalline glucose,
- soluble dietary fiber

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References (19)

References

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The Preparation of High-purity Crystalline Glucose and Soluble Dietary Fiber through Enzymolysis of Sweet Potato Residue

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