The HSPD1 expression in EA.hy926 cells apoptosis induced by hight glucose and hight cholestrol

To explore the expression of HDPD1 gene in EA.hy926 cell apoptosis induced by high glucose and hight cholesterol. Methods: cells were incubated with high or low concentrations of glucose (25 mmol/L ) and cholestrol (0.8 , 1.6 ,3.2 mmol/L) for different times. We detected the cell activity by CCK8 Kit, observed the cell apoptosis by FACS (Flow Cytometer), and measured the expression of HSPD1 gene by RT-PCR and QPCR. Results: Cholesterol (0.8 , 1.6 , 3.2 mmol/L) together with high glucose strikingly decresed cell activity and in a dose-dependent manner, the same as the cholesterol (1.6 , 3.2 mmol/L) together with low glucose, but not for the cholestrol (0.8 mmol/L) with low glucose. The cell activity also decreased in a time dependent manner when treated with low or high glucose + 0.8 mmol/L. Most of cells treated with hight glucose and hight cholesterol (high glucose + 0.8 mmol/L cholesterol) underwent apoptosis compared with the low glucose and high cholesterol (high glucose + 0.8 mmol/L cholesterol). Meanwhile, the expression of HSPD1 gene were dramatically induced by high glucose and high cholesterol.