陈静, 陈芸, 热依麦阿依·阿布都艾尼, 方志刚, 凯迪日耶·玉苏普, 马刘峰. 月季插穗不定根起始的转录组分析和关键基因筛选[J]. 华南师范大学学报(自然科学版), 2021, 53(3): 54-62. doi: 10.6054/j.jscnun.2021044
引用本文: 陈静, 陈芸, 热依麦阿依·阿布都艾尼, 方志刚, 凯迪日耶·玉苏普, 马刘峰. 月季插穗不定根起始的转录组分析和关键基因筛选[J]. 华南师范大学学报(自然科学版), 2021, 53(3): 54-62. doi: 10.6054/j.jscnun.2021044
CHEN Jing, CHEN Yun, Reyimaiayi·ABUDUAINI, FANG Zhigang, Kaidiriye·YUSUPU, MA Liufeng. A Transcriptomic Analysis of Early Adventitious Roots of Rosa chinensis Cuttings and Key Genes Screening[J]. Journal of South China Normal University (Natural Science Edition), 2021, 53(3): 54-62. doi: 10.6054/j.jscnun.2021044
Citation: CHEN Jing, CHEN Yun, Reyimaiayi·ABUDUAINI, FANG Zhigang, Kaidiriye·YUSUPU, MA Liufeng. A Transcriptomic Analysis of Early Adventitious Roots of Rosa chinensis Cuttings and Key Genes Screening[J]. Journal of South China Normal University (Natural Science Edition), 2021, 53(3): 54-62. doi: 10.6054/j.jscnun.2021044

月季插穗不定根起始的转录组分析和关键基因筛选

A Transcriptomic Analysis of Early Adventitious Roots of Rosa chinensis Cuttings and Key Genes Screening

  • 摘要: 为研究月季插穗在不定根发生过程中的关键基因调控机理,利用Illumina平台测序技术对切花月季品种‘卡罗拉’插穗的3个发育阶段(不定根未启动期、愈伤组织形成期和不定根伸长期)插穗基部1 cm皮层进行转录组测序分析,结果表明:月季插穗不定根发生的3个阶段中,在不定根未启动期与愈伤组织形成期之间共筛选出差异表达基因5 033个,其中2 313个基因上调,2 720个基因下调;在愈伤组织形成期与不定根伸长期之间共筛选出差异表达基因1 865个,其中1 332个基因上调,533个基因下调;GO功能分析表明,差异表达基因主要参与生物过程、分子功能和细胞组分3大功能;KEGG富集分析结果表明,差异表达基因主要参与植物激素信号转导、次生代谢产物的合成以及碳水化合物的合成等代谢通路;将月季插穗生根过程中差异性最为显著的8个基因通过实时荧光定量PCR检测其转录水平变化,结果表明: 实时荧光定量PCR的验证结果与转录组测序结果基本一致.

     

    Abstract: In order to study the key gene regulation mechanism of Rosa chinensis cuttings in the process of adventitious root formation, the Illumina platform sequencing technology was used to analyze the three developmental stages of cutting rose cultivar 'Carola', i.e., adventitious root initiation period, callus formation period and adventitious root elongation period. A transcriptome sequencing analysis was performed on the 1 cm cortex at the base of cuttings. The results showed that at the three stages of adventitious root formation of rose cuttings, a total of 5 033 differentially expressed genes were screened between the adventitious root initiation stage and the callus formation stage, of which 2 313 genes were up-regulated and 2 720 genes were down-regulated; a total of 1 865 differentially expressed genes were screened between the callus formation period and adventitious root elongation period, of which 1 332 genes were up-regulated and 533 genes were down-regulated. The GO functional analysis showed that differentially expressed genes were mainly involved in the 3 major functions of biological process, molecule function and cell composition. The KEGG enrichment analysis showed that the differentially expressed genes were mainly involved in the metabolic pathways of plant hormone signal transduction, the synthesis of secondary metabolites and the synthesis of carbohydrates. The changes in the transcription levels of the 8 genes that exhibited the most significant differences in the rooting process were detected with real-time fluorescent quantitative PCR. The results showed that the verification results of real-time fluorescent quantitative PCR were basically consistent with the results of transcriptome sequencing.

     

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