Abstract: Inhibitions of the exopolysaccharide extract from Morchella conica on superoxide anion, hydroxyl radical and ABTS free radical were tested for evaluating the in-vitro-antioxidant activity. And through gastric perfusion to aging model mice, effects of the extract on superoxide dismutase activity, content of malondialdehyde and lipofuscin in brains of mice were studied. The results showed that the exopolysaccharide extract inhibited superoxide anion by 43.06% at 2mg/ml, scavenged hydroxyl radical by 72.4% at 1mg/ml and ABTS free radical by 77.43% at 0.1mg/ml. And at 50 mg/kg?d of the extract, SOD activities of male and female mice were increased by 20.70% and 21.13% respectively, while contents of LF and MDA were decreased 35.14% and 46.93% , 15.10% and 15.61% . It suggests that the exopolysaccharide extract from M. conica has obviously antioxidative and antiaging effects,and had gender differences on the antiaging effects, expressing a superior effect on female mice.