Abstract: Cu(sal--Ala)(3,5-DMP2) is modified on the surface of the single-walled carbon nanotubes (SWCNTs) glass carbon electrode (GCE). The modified electrode shows impressive detection ability of dopamine (DA) and uric acid (UA). The modified electrode has a good electrocatalytic effect on DA and UA. The detection linear range of Cu(sal--Ala)(3,5-DMP2) to DA is 10.00 to 210 mmol/L and the detection limit is 7.29 mol/L. While, the detection linear range of Cu(sal--Ala)(3,5-DMP2) to UA has a good linear range from 1 to 86 mmol/L and the detection limit is 1.5 mol/L. In this work Simultaneous Differential Pulse Voltammetric (DPV) is employed to determine DA and UA. DPV method with Cu(sal--Ala)(3,5-DMP2)/SWCNTs/GCE has a good sensitivity and resolution rate.