Abstract: Though many studies about the hybridization between teosinte and closely related species of maize have been reported, little research about tissue culture of teosinte is reported. Here, establishment of technique system of tissue culture for teosinte was made by using the?variety 8493 of? Zea?mays?spp.mexicana?L as explants. The high totipotency swelling of the cultured internode was induced from teosinte seeds in the MS culture medium added 2,4-D and 6-BA and subcultured in the dark for induction of callus of teosinte about 2 weeks. The callus are authenticated to embryonic callus by paraffin section observation.