张粹兰, 张名位, 廖森泰, 魏振承, 张雁, 唐小俊, 张瑞芬, 邓媛元. 荔枝果肉不同极性多酚提取及其抗氧化活性比较[J]. 华南师范大学学报(自然科学版), 2011, (4).
引用本文: 张粹兰, 张名位, 廖森泰, 魏振承, 张雁, 唐小俊, 张瑞芬, 邓媛元. 荔枝果肉不同极性多酚提取及其抗氧化活性比较[J]. 华南师范大学学报(自然科学版), 2011, (4).
COMPARISON OF THE CONTENT AND ANTIOXIDANT ACTIVITIES OF DIFFERENT POLARITY PHENOLICS OF LITCHI PULP[J]. Journal of South China Normal University (Natural Science Edition), 2011, (4).
Citation: COMPARISON OF THE CONTENT AND ANTIOXIDANT ACTIVITIES OF DIFFERENT POLARITY PHENOLICS OF LITCHI PULP[J]. Journal of South China Normal University (Natural Science Edition), 2011, (4).

荔枝果肉不同极性多酚提取及其抗氧化活性比较

COMPARISON OF THE CONTENT AND ANTIOXIDANT ACTIVITIES OF DIFFERENT POLARITY PHENOLICS OF LITCHI PULP

  • 摘要: 为了了解荔枝果肉多酚的含量及其抗氧化活性,分别选用60%乙醇和80%丙酮从荔枝果肉中提取多酚,并按极性大小对其多酚进行分部萃取,采用DPPH、FRAP和ABTS法比较不同极性多酚的体外抗氧化活性差异.结果表明,采用80%丙酮提取的荔枝果肉多酚的抗氧化活性显著强于60%乙醇提取的(P0.05),两种提取法所获得的不同极性多酚含量都存在显著差异(P0.05),其中水部多酚含量最高,正丁醇部次之;四种极性多酚的抗氧化活性亦存在显著差异(P0.05),以乙酸乙酯部活性最强,正丁醇部其次(P0.05).通过对不同极性荔枝果肉多酚的有效分离和抗氧化活性比较,为荔枝果肉多酚的分离纯化和抗氧化机制研究奠定了基础.

     

    Abstract: In order to investigate phenolics and their antioxidant activities of litchi pulp, the paper used 60% ethanol and 80% acetone to extract polyphenol from litchi chinensis pulp separately. The polyphenols were separated according to the polarity separation size, which was combined with DPPH, FRAP and ABTS to evaluate antioxidant activity of different polarity polyphenols. The results showed that antioxidant activity of the litchi pulp polyphenol extract by 80% acetone extract was significantly stronger than the 60% ethanol(P0.05). Polyphenol content of different polarity were significantly different (P0.05). The water layer had the highest polyphenol content, which was followed by the n-butyl alcohol part. The antioxidant activity of different polar solvent polyphenols were significantly different (P0.05), which the ethyl acetate showed the highest antioxidant activity , followed by n-butyl alcohol part. The activity of acetone extract polyphenol was significantly higher than the ethanol extract of polyphenols (P0.05). Through the effective separation of different polarity polyphenols and evaluation of antioxidant activity, the study formed the foundation for the research of the highly active polyphenols of litchi pulp.

     

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