Coupling Reaction and Application of Jasmonic Acid And Cdte Quabtum Dots

As a good luminescent material，quantum dot can be a probe for targets in complicated system through the surface modification with recognition function molecule. CdTe quantum dots(QDs) were synthesized in aqueous solution using mercaptoethylamine(MA) as stabilizer. The optimal conditions for synthesis were studied by fluorescence spectroscopy. The purification of CdTe QDs with different solvents and pH values of buffer solution was investigated systematically. The results showed that the purification with twice volume isopropanol was the best.The fluorescence of QDs basically unchanged in pH 7. 4 buffer solution. The jasmonic acid-CdTe conjugates was obtained by coupling CdTe QDs with jasmonic acid(JA) using the Nhydroxysuccinimide(NHS) as a coupling agent. The optimal conditions for coupling reaction are pH 7. 4 and stiring for 30 min at 370C .After the JA molecule linked with CdTe QDs，the maximum absorption peak of jasmonic acid-CdTe conjugates was blue-shifted by 10 nm.The photostability of jasmonic acid-CdTe conjugates was also studied by fluorescence microscope. The jasmonic acid一CdTe conjugates was applied preliminarily in the labeling of root sections of mung bean seedlings.