The Antibacterial Effect of 82 Kinds of Chinese Herbal Medicines and Their Compounds on <i>Aeromonas vernoii</i> from <i>Lateolabrax maculatus</i> in Vitro

WANG Baotun; YAN Yuanyi; MAO Can; FENG Juan; DENG Yiqin; SU Youlu

doi:10.6054/j.jscnun.2021043

Journal of South China Normal University (Natural Science Edition) > 2021 > 53(3): 64-72. > DOI: 10.6054/j.jscnun.2021043

WANG Baotun, YAN Yuanyi, MAO Can, FENG Juan, DENG Yiqin, SU Youlu. The Antibacterial Effect of 82 Kinds of Chinese Herbal Medicines and Their Compounds on Aeromonas vernoii from Lateolabrax maculatus in Vitro[J]. Journal of South China Normal University (Natural Science Edition), 2021, 53(3): 64-72. DOI: 10.6054/j.jscnun.2021043

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The Antibacterial Effect of 82 Kinds of Chinese Herbal Medicines and Their Compounds on Aeromonas vernoii from Lateolabrax maculatus in Vitro

WANG Baotun^{1, 2},
YAN Yuanyi⁴,
MAO Can^{1, 2},
FENG Juan²,
DENG Yiqin²,
SU Youlu^{2, 3, ,}

1.
National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
2.
South China Sea Fishery Research Institute, Chinese Academy of Fishery Sciences//Key Laboratory of South China Sea Fishery Resources Development and Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China
3.
Institute of Healthy Animal Breeding Innovation, Zhongkai University of Agriculture and Engineering, Guangzhou 501225, China
4.
Animal Husbandry Technology Popularization station of GuangDong, Guangzhou 501500, China

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Received Date: June 28, 2020
Available Online: July 05, 2021

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Abstract

Abstract

To study the antibacterial effect of single and compound Chinese herbal medicines on Aeromonas vernoii from Lateolabrax maculatus in vitro, the inhibitory effect of 82 kinds of single Chinese herbal medicine, such as Prunus mume, Caesalpinia sappan and Galla chinensis, on A. vernoii was determined with the plate drilling method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of Chinese herbal medicine with good antibacterial effect on A. vernoii were determined with the testing tube double dilution method. The more sensitive Chinese herbal medicines were selected to evaluate their compound antibacterial effect. The results showed that 36 kinds of single Chinese herbal medicines had inhibitory effects on A. vernoii, among which P. mume, C. sappan, G. chinensis, Syzygium aromaticum and Artemisia argyi had relatively strong inhibitory effects on A. vernoii. The diameter of the inhibitory circle was between 15~25 mm, MIC < 125 mg/mL and MBC < 500 mg/mL. Among the compound Chinese herbal medicines, P. mume+S. aromaticum and P. mume+G. chinensis had better inhibitory effect on A. vernoii. The inhibitory circle diameter was >19 mm, MIC was 15.6 mg/mL, and MBC was 31.25~62.5 mg/mL. However, the combined bacteriostatic index analysis showed that they had an independent effect. This study shows that single Chinese herbal medicine such as P. mume and C. sappan were beneficial to the inhibition and elimination of A. vernoii in vitro and provides data support for the development of aquatic Chinese herbal medicine.
- Lateolabrax maculatus,
- Aeromonas vernoii,
- Chinese herbal medicine,
- antibacterial effect

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