Macrophage's Promotion of Cervical Cancer Cell Resistance to SN-38

GDSC (Genomics of Drug Sensitivity in Cancer) online database was analyzed and the IC₅₀ Z Score values of 8 cervical cancer cell lines under more than 310 chemotherapy drugs were obtained. Then HPV-18 positive HeLa cells, HPV-16 positive SiHa and CaSki cells and HPV negative C-33A cells were treated with SN-38 for 24 and 48 hours and the cell viability was detected with CCK8 experiment. Finally, THP1 cells were induced into tumor-associated macrophages (TAM) to explore the role of TAM in the sensitivity of cervical cancer cells to SN-38. The results showed that CaSki, ME-180, HT-3 and C-33A were very sensitive to SN-38, HeLa and SiHa were relatively sensitive to SN-38, and CAL-39 was not sensitive to SN-38. In addition, IC₅₀ values of CaSki and C-33A were lower than those of HeLa and SiHa, indicating that CaSki and C-33A were more sensitive to SN-38. TAM promoted HeLa and SiHa cell resistance to SN-38. In conclusion, the results suggested that TAM promoted the resistance of cervical cancer cells to SN-38.