Preparation of Microspheres for Molecularly Imprinted Surface Solid Phase Extraction and its Application in the Separation and Detection of Glutamic Acid

HE Peiling; CHEN Shiyuan; HU Xiaogang

doi:10.6054/j.jscnun.2019061

Journal of South China Normal University (Natural Science Edition) > 2019 > 51(4): 32-38. > DOI: 10.6054/j.jscnun.2019061

HE Peiling, CHEN Shiyuan, HU Xiaogang. Preparation of Microspheres for Molecularly Imprinted Surface Solid Phase Extraction and its Application in the Separation and Detection of Glutamic Acid[J]. Journal of South China Normal University (Natural Science Edition), 2019, 51(4): 32-38. DOI: 10.6054/j.jscnun.2019061

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Preparation of Microspheres for Molecularly Imprinted Surface Solid Phase Extraction and its Application in the Separation and Detection of Glutamic Acid

School of Chemistry and Environment, South China Normal University, Guangzhou, 510006, China

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Received Date: October 22, 2018
Available Online: March 21, 2021

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Abstract

Abstract

Molecularly imprinted solid-phase extraction (MISPE) microspheres were prepared using L-glutamic acid, 2-acrylamido-2-methyl propane sulfonic acid and N, N-methylene bis-acrylamide as template, functional monomer and cross-linker respectively and using the reversible addition-fragmentation chain transfer polymerization. Then the imprinted silica gel microspheres were characterized with Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and thermogravimetric analysis (TG). The results of the extraction experiment showed that MIPs exhibited excellent performance in separating glutamic acid from soy sauce, and an extraction capacity up to 140 μg/g was achieved. The rapid analysis of L-glutamic was realized by combining MISPE with high performance liquid chromatography technology. The linearity of L-glutamic acid was 1.47~58.9 μg/mL and the detection limit was 44.1 ng/mL. The recovery rate was 77.8%~82.2%.
- molecular imprinting,
- solid-phase extraction,
- high performance liquid chromatography,
- L-glutamic acid,
- soy sauce

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References (17)

References

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Preparation of Microspheres for Molecularly Imprinted Surface Solid Phase Extraction and its Application in the Separation and Detection of Glutamic Acid

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