蒙脱石K-10固定糖化酶研究

Study on the Immobilization of Glucoamylase on Montmorillonite K-10

摘要: 蒙脱石是一种优良的吸附剂，采用吸附法将其作为固定化糖化酶的载体研究固定化糖化酶的性质，结果表明：每克固定化酶的活力3442U；固定化酶的最适pH值是4.8，比游离酶增加了0.2个单位；固定化酶反应的最适温度是60℃，比游离酶升高了5℃；固定化酶的半衰期为12d；固定化酶的Km=14.3g/L，为游离酶的1.47倍.

Abstract: The montmorillonite is a good absorptive and is used as a carrier for glucoamylase immobilization by absorption. The results here show that the activity of immobilized enzyme is 3442u per gram；the optimal pH value of immobilized enzyme is 4.8, 0.2 higher than that of free enzyme；the optimal temperature is 60℃, 5℃ higher than free one； the half-life of immobilized enzyme is 12 days; and the Michaelis constant of immobilized enzyme is Km=14.3g/L, which is 1.47 times as the free one.