张玉丽, 帅祥煜, 梁飞, 齐园圃, 张丽美, 闫旭洁, 梁伟宝, 王松涛. 累积运动对胰岛素抵抗小鼠心肌胶原纤维的影响[J]. 华南师范大学学报(自然科学版), 2023, 55(2): 73-79. doi: 10.6054/j.jscnun.2023022
引用本文: 张玉丽, 帅祥煜, 梁飞, 齐园圃, 张丽美, 闫旭洁, 梁伟宝, 王松涛. 累积运动对胰岛素抵抗小鼠心肌胶原纤维的影响[J]. 华南师范大学学报(自然科学版), 2023, 55(2): 73-79. doi: 10.6054/j.jscnun.2023022
ZHANG Yuli, SHUAI Xiangyu, LIANG Fei, QI Yuanpu, ZHANG Limei, YAN Xujie, LIANG Weibao, WANG Songtao. The Effect of Accumulated Exercise on Myocardial Collagen Fibers in Insulin Resistant Mice[J]. Journal of South China Normal University (Natural Science Edition), 2023, 55(2): 73-79. doi: 10.6054/j.jscnun.2023022
Citation: ZHANG Yuli, SHUAI Xiangyu, LIANG Fei, QI Yuanpu, ZHANG Limei, YAN Xujie, LIANG Weibao, WANG Songtao. The Effect of Accumulated Exercise on Myocardial Collagen Fibers in Insulin Resistant Mice[J]. Journal of South China Normal University (Natural Science Edition), 2023, 55(2): 73-79. doi: 10.6054/j.jscnun.2023022

累积运动对胰岛素抵抗小鼠心肌胶原纤维的影响

The Effect of Accumulated Exercise on Myocardial Collagen Fibers in Insulin Resistant Mice

  • 摘要: 累积运动是打破久坐行为和改善代谢健康的有效手段。为了探究累积运动的健康干预价值,文章通过对胰岛素抵抗(IR)小鼠进行长期的运动干预,比较了不同强度的累积运动和持续运动对小鼠IR状态、心肌胶原纤维和心肌纤维化蛋白表达的影响。首先,高脂饲料喂养112只C57BL/6J雄性小鼠(4周龄)诱导IR小鼠模型;然后,用中等强度持续运动、中等强度累积运动和大强度累积运动干预IR小鼠8周,进行口服葡萄糖耐量实验(OGTT),眼眶取血后处死小鼠, 摘取其心脏组织;其次,使用ELISA方法检测小鼠血清空腹胰岛素(FINS)水平和心肌组织Ⅰ型胶原(COLⅠ)、Ⅲ型胶原(COLⅢ)、平滑肌肌动蛋白(α-SMA)含量;使用Western blot方法检测小鼠心肌组织转化生长因子β1(TGF-β1)、Smad3、结缔组织生长因子(CTGF)蛋白表达水平。结果显示:3种运动均可以改善IR小鼠的IR状态和心肌纤维化程度,大强度累积运动与中等强度持续运动的作用效果相当,中等强度累积运动的作用效果较弱。

     

    Abstract: Accumulated exercise is an effective means for breaking sedentary behavior and improving metabolic health. To explore the health intervention value of accumulated exercise, the effects of different intensities of accumulated exercise and continuous exercise have been compared on insulin resistance (IR) status, myocardial collagen fibers, and myocardial fibrosis protein expression in mice through long-term exercise. The IR mouse model was induced by feeding 112 C57BL/6J male mice (4 weeks old) with high-fat diet. The IR model mice were intervened with moderate intensity continuous exercise, moderate intensity accumulated exercise and high intensity accumulated exercise for 8 weeks. Oral glucose tolerance test(OGTT) was performed, and the mice were sacrificed after blood was collected from the orbit to extract heart tissue. The ELISA method was used to detect serum Fasting insulin(FINS) level, myocardial tissue collagen Ⅰ(COLⅠ), collagen Ⅲ(COLⅢ) and α-smooth muscle actin (α-SMA) content. The Western blot method was used to detect mouse myocardial tissue transforming growth factor-β1(TGF-β1), Smad3 and connective tissue growth factor(CTGF) protein expression level. The results showed that all three kinds of exercise could improve the IR state and myocardial fibrosis degree of IR mice, the effects of high intensity accumulated exercise and moderate intensity continuous exercise were equivalent, while the effect of mode-rate intensity accumulated exercise was weaker.

     

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