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白藜芦醇上调GDF-15调控PI3K/Akt/Bcl-2信号通路对急性心肌梗死大鼠的保护作用

颜仁梁 夏黎 周国洪 沈小钟

颜仁梁, 夏黎, 周国洪, 沈小钟. 白藜芦醇上调GDF-15调控PI3K/Akt/Bcl-2信号通路对急性心肌梗死大鼠的保护作用[J]. 华南师范大学学报(自然科学版), 2022, 54(2): 76-82. doi: 10.6054/j.jscnun.2022029
引用本文: 颜仁梁, 夏黎, 周国洪, 沈小钟. 白藜芦醇上调GDF-15调控PI3K/Akt/Bcl-2信号通路对急性心肌梗死大鼠的保护作用[J]. 华南师范大学学报(自然科学版), 2022, 54(2): 76-82. doi: 10.6054/j.jscnun.2022029
YAN Renliang, XIA Li, ZHOU Guohong, SHENG Xiaozhong. The Myocardial Preservation Effect of Resveratrol by Up-Regulating GDF-15 to Activate PI3K/Akt/Bcl-2 Pathway in Acute Myocardial Infarction Rats[J]. Journal of South China normal University (Natural Science Edition), 2022, 54(2): 76-82. doi: 10.6054/j.jscnun.2022029
Citation: YAN Renliang, XIA Li, ZHOU Guohong, SHENG Xiaozhong. The Myocardial Preservation Effect of Resveratrol by Up-Regulating GDF-15 to Activate PI3K/Akt/Bcl-2 Pathway in Acute Myocardial Infarction Rats[J]. Journal of South China normal University (Natural Science Edition), 2022, 54(2): 76-82. doi: 10.6054/j.jscnun.2022029

白藜芦醇上调GDF-15调控PI3K/Akt/Bcl-2信号通路对急性心肌梗死大鼠的保护作用

doi: 10.6054/j.jscnun.2022029
基金项目: 

国家自然科学基金项目 81803693

广东省医学科研基金项目 B2020063

广东省教育厅科研课题 2021KTSCX242

广东省中医药局科研项目 20221290

详细信息
    通讯作者:

    颜仁梁,Email: yanrl@gdyzy.edu.cn

  • 中图分类号: R285.5

The Myocardial Preservation Effect of Resveratrol by Up-Regulating GDF-15 to Activate PI3K/Akt/Bcl-2 Pathway in Acute Myocardial Infarction Rats

  • 摘要: 为考察白藜芦醇对心肌缺血的保护机制,采用冠状动脉前降支结扎再灌注制备大鼠心肌缺血再灌注(I/R)模型,运用试剂盒考察血清酶学指标、NBT染色考察心肌梗死率、蛋白质印迹法(Western blotting) 考察GDF-15、PI3K、Akt、p-Akt、Bcl-2、Bax、caspase-9和caspase-3的蛋白相对表达量。结果表明:白藜芦醇对心肌缺血再灌注大鼠的心肌保护作用与通过对GDF-15的上调来激活PI3K/Akt信号通路,抑制心肌细胞线粒体凋亡作用机制有关。
  • 图  1  白藜芦醇对大鼠心肌缺血再灌注后心肌梗死率的影响(n=10,x±s)

    注:与假手术组比较:##表示P<0.01;与模型组比较:*表示P<0.05。

    Figure  1.  The effect of resveratrol on myocardial infarction rate after myocardial ischemia-reperfusion in rats(n=10, x±s)

    图  2  白藜芦醇对缺血再灌注大鼠心肌组织的GDF-15、PI3K和p-Akt相对表达量的影响(n=10,x±s)

    注:与假手术组比较:##表示P<0.01;与模型组比较:*表示P<0.05,**表示P<0.01。

    Figure  2.  The effects of resveratrol on the activities of CK-MB, LDH, SOD and the content of MDA in serum of ischemia-reperfusion rats and on the relative expression of GDF-15, PI3K and p-Akt in myocardial tissue of ischemia-reperfusion rats(n=10, x±s)

    图  3  白藜芦醇对缺血再灌注大鼠心肌组织的Bax/Bcl-2和caspase蛋白相对表达量的影响(n=10,x±s)

    注:与假手术组比较:##表示P<0.01;与模型组比较:*表示P<0.05。

    Figure  3.  The effect of resveratrol on the relative expression of Bax / Bcl-2 and caspase in myocardial tissue of ischemia-reperfusion rats(n=10, x±s)

    表  1  白藜芦醇对缺血再灌注大鼠血清中CK-MB、LDH、SOD活力和MDA含量的影响(n=10,x±s)

    Table  1.   The effects of resveratrol on the activity of CK-MB, LDH, SOD and the content of MDA in serum of ischemia-reperfusion rats(n=10, x±s)

    组别 剂量/(mg·kg-1) CK-MB/(U·L-1) LDH/(U·L-1) MDA/(nmol·mL-1) SOD/(U·L-1)
    假手术组 343.88±55.13 1 301.63±429.74 10.32±1.65 49.57±7.17
    模型组 702.95±51.06## 2 253.62±817.62## 21.09±4.53## 30.99±6.89##
    普萘洛尔组 1 396.11±85.38** 1 340.22±717.34** 11.88±2.56** 45.60±9.10*
    白藜芦醇组 5 633.17±42.42 2 045.89±693.46 19.42±4.40 31.53±6.70
    10 506.78±34.67* 1 936.96±492.67 15.20±4.04* 37.87±2.51
    20 367.67±77.79** 1 250.00±286.58* 13.33±2.25** 41.90±6.46*
    注:与假手术组比较:##表示P<0.01;与模型组比较:*表示P<0.05,**表示P<0.01。
    下载: 导出CSV
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出版历程
  • 收稿日期:  2021-05-13
  • 网络出版日期:  2022-05-12
  • 刊出日期:  2022-04-25

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