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熊果苷纳米粒的制备及用于烧伤皮肤修复研究

林晓霞 何应畅 尤蓉

林晓霞, 何应畅, 尤蓉. 熊果苷纳米粒的制备及用于烧伤皮肤修复研究[J]. 华南师范大学学报(自然科学版), 2021, 53(5): 62-67. doi: 10.6054/j.jscnun.2021076
引用本文: 林晓霞, 何应畅, 尤蓉. 熊果苷纳米粒的制备及用于烧伤皮肤修复研究[J]. 华南师范大学学报(自然科学版), 2021, 53(5): 62-67. doi: 10.6054/j.jscnun.2021076
LIN Xiaoxia, HE Yingchang, YOU Rong. The Preparation of Arb-BSA-NP Nanoparticals and Their Application to Burn Injury Repair[J]. Journal of South China normal University (Natural Science Edition), 2021, 53(5): 62-67. doi: 10.6054/j.jscnun.2021076
Citation: LIN Xiaoxia, HE Yingchang, YOU Rong. The Preparation of Arb-BSA-NP Nanoparticals and Their Application to Burn Injury Repair[J]. Journal of South China normal University (Natural Science Edition), 2021, 53(5): 62-67. doi: 10.6054/j.jscnun.2021076

熊果苷纳米粒的制备及用于烧伤皮肤修复研究

doi: 10.6054/j.jscnun.2021076
基金项目: 

广东省自然科学基金项目 2021A1515010775

广东省特色创新项目 2016KTSCX023

详细信息
    通讯作者:

    尤蓉,Email:yourong@scnu.edu.cn

  • 中图分类号: Q819

The Preparation of Arb-BSA-NP Nanoparticals and Their Application to Burn Injury Repair

  • 摘要: 通过以牛血清白蛋白V(BSA)为载体,去溶剂化法制备熊果苷纳米粒(Arb-BSA-NP),研究其对大鼠烧伤修复的效果. 利用粒径分布和Zeta电位对纳米粒进行表征,结果显示:熊果苷与白蛋白比例1 ∶ 10、pH7.0的条件下用戊二醛交联12 h,粒子大小均一且稳定性较好,平均粒径为175.26±11.04 nm,Zeta电位为-29.12±0.80 mV;将细菌纤维素膜浸渍于含Arb-BSA-NP纳米粒溶液中获得负载熊果苷纳米粒的纤维膜敷料(Arb-BSA-BC),并对该材料进行SEM分析,显示Arb-BSA-NP纳米颗粒分布在材料表面;通过SD大鼠烧伤模型动物实验评价该敷料对烧伤创面愈合的效果,结果显示负载熊果苷纳米粒的纤维膜敷料有助于烧伤创面的恢复和愈合.
  • 图  1  烧伤实验手术过程示意图

    Figure  1.  The sketch map of the experiment of ratburn injury repair

    图  2  不同投料比对熊果苷纳米粒粒径及Zeta电位分布的影响

    Figure  2.  The effects of different ratios of arbutin to albumin on Arb-BSA-NP size and Zeta potential

    图  3  不同pH对熊果苷纳米粒粒径及Zeta电位分布的影响

    Figure  3.  The effects of different pH values on Arb-BSA-NP particle size and zeta potential

    图  4  不同交联时间对熊果苷纳米粒粒径及Zeta电位分布的影响

    Figure  4.  The effects of different cross-linkage time lengths on Arb-BSA-NP size and Zeta potential

    图  5  负载Arb-BSA-NP的细菌纤维素和无负载纳米粒细菌纤维素的SEM分析

    Figure  5.  The SEM analysis of cellulose loaded with Arb-BSA-NP and cellulose with no precipitation of nanoparticals

    图  6  SD大鼠烧伤创面的愈合情况

    Figure  6.  The healing of rat burn injury under different treatments

    图  7  SD大鼠烧伤创面的病理切片(HE染色,×100)

    Figure  7.  The histological section of rat burn injury

  • [1] 王慧琛, 王丽琴. 熊果苷的应用及检测研究进展[J]. 天津药学, 2012, 24(4): 71-74. doi: 10.3969/j.issn.1006-5687.2012.04.033

    WANG H S, WANG L Q. Development of arbutin's application and detection[J]. Tianjin Pharmacy, 2012, 24(4): 71-74. doi: 10.3969/j.issn.1006-5687.2012.04.033
    [2] 简龙海, 袁晓倩, 郑荣, 等. 高效液相色谱-荧光检测法同时测定化妆品中的α-熊果苷, β-熊果苷, 氢醌和苯酚[J]. 药物分析杂志, 2020, 40(2): 273-277. https://www.cnki.com.cn/Article/CJFDTOTAL-YWFX202002012.htm

    JIAN L H, YUAN X Q, ZHENG R, et al. Determination of α-arbutin, β-arbutin, hydroquinone and phenol in cosmetics by high performance liquid chromatography-fluorescent detection[J]. Chinese Journal of Pharmacy Analysis, 2020, 40(2): 273-277. https://www.cnki.com.cn/Article/CJFDTOTAL-YWFX202002012.htm
    [3] BARSOOM B N, ABDELSAMAD A M, ADIB N M. Indirect spectrophotometric determination of arbutin, whitening agent through oxidation by periodate and complexation with ferric chloride[J]. Spectrochimica Acta Part A: Molecular & Biomolecular Spectroscopy, 2006, 64(4): 844-852.
    [4] 王佩, 赖瑛, 吴锡铭. 熊果苷抗炎作用的研究[J]. 中华中医药学刊, 2008, 26(9): 1933-1935. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYHS200809037.htm

    WANG P, LAI Y, WU X M. Research on anti-inflammation effects of Arbutin[J]. Chinese Archives of Traditional Chinese Medicine, 2008, 26(9): 1933-1935. https://www.cnki.com.cn/Article/CJFDTOTAL-ZYHS200809037.htm
    [5] 唐婕妤, 彭菲. 熊果苷的药理作用与资源获取途径研究进展[J]. 今日药学, 2015(9): 673-677. https://www.cnki.com.cn/Article/CJFDTOTAL-YAXU201509020.htm

    TANG J Y, PENG F. Research progress of pharmacological effects and ways of obtaining resources of Arbuti[J]. Pharmacy Today, 2015(9): 673-677. https://www.cnki.com.cn/Article/CJFDTOTAL-YAXU201509020.htm
    [6] 蒋秋萍, 石玉岚, 黄磊, 等. 轻度烧伤治疗中临床护理路径的应用及效果分析[J]. 感染·炎症·修复, 2020(1): 10, 15. https://www.cnki.com.cn/Article/CJFDTOTAL-GRYX202001004.htm

    JIANG Q P, SHI Y L, HUANG L, et al. Application and effect analysis of clinical nursing pathway in the treatment of mild burn[J]. Infection, Inflammation, Repair, 2020(1): 10, 15. https://www.cnki.com.cn/Article/CJFDTOTAL-GRYX202001004.htm
    [7] DUGAN J M, GOUGH J E, EICHHORN S J. Bacterial cellulose scaffolds and cellulose nanowhiskers for tissue engineering[J]. Nanomedicine, 2013, 8(2): 287-298. doi: 10.2217/nnm.12.211
    [8] 张崧, 王玉海, 石光, 等. 细菌纤维素/TiO2锂离子电池复合隔膜的研究[J]. 华南师范大学学报(自然科学版), 2017, 49(2): 21-27. doi: 10.6054/j.jscnun.2017091

    ZHANG S, WANG Y H, SHI G, et al. Study on bacterial cellulose/TiO2 composite separators for lithium-ion batteries[J]. Journal of South China Normal University (Natural Science Edition), 2017, 49(2): 21-27. doi: 10.6054/j.jscnun.2017091
    [9] JAHANBAN-ESFAHLAN A, DASTMALCHI S, DAVAR-AV S, et al. A simple improved desolvation method for the rapid preparation of albumin nanoparticles[J]. International Journal of Biological Macromolecules, 2016, 91: 703-709. doi: 10.1016/j.ijbiomac.2016.05.032
    [10] 鞠学鹏, 孙明立, 赵海山, 等. 应用膏状固体酒精灼烧法建立一种新的大鼠烧伤模型[J]. 沈阳药科大学学报, 2008(S1): 133-134. https://www.cnki.com.cn/Article/CJFDTOTAL-SYYD2008S1155.htm

    JU X P, SUN M L, ZHAO H S, et al. A new model of rat burn injury established by burning with paste solid alcohol[J]. Journal of Shenyang Pharmaceutical University, 2008(S1): 133-134. https://www.cnki.com.cn/Article/CJFDTOTAL-SYYD2008S1155.htm
    [11] TERCINIER L, YE A, SINGH A, et al. Effects of ionic strength, pH and milk serum composition on adsorption of milk proteins on to hydroxyapatite particles[J]. Food Biophysics, 2014, 9(4): 341-348. doi: 10.1007/s11483-014-9360-5
    [12] BRAILE M C, CARNEVALLI N C, GOISSIS G, et al. In vitro properties and performance of glutaraldehyde-crosslinked bovine pericardial bioprostheses treated with glutamic acid[J]. Artificial Organs, 2011, 35(5): 497-501. doi: 10.1111/j.1525-1594.2011.01262.x
    [13] LEE H J, KIM K W. Anti-inflammatory effects of arbutin in lipopolysaccharide-stimulated BV2 microglial cells[J]. Inflammation Research, 2012, 61(8): 817-825. doi: 10.1007/s00011-012-0474-2
    [14] XU W H, LIANG Q, ZHANG Y J, et al. Naturally occurring arbutin derivatives and their bioactivities[J]. Chemistry and Biodiversity, 2015, 12(1): 54-81.
    [15] RAJWADE J M, PAKNIKAR K M, KUMBHAR J V. Applications; of bacterial cellulose and its composites in biomedicine[J]. Applied Microbiology and Biotechnology, 2015, 99(6): 2491-2511.
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出版历程
  • 收稿日期:  2020-10-05
  • 网络出版日期:  2021-11-11
  • 刊出日期:  2021-10-25

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