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基于苯并呋咱胺类衍生物的碱性荧光探针

张中岩 刘诗莹 许金松 卢娉婷 刘欣 李煦田 马立军

张中岩, 刘诗莹, 许金松, 卢娉婷, 刘欣, 李煦田, 马立军. 基于苯并呋咱胺类衍生物的碱性荧光探针[J]. 华南师范大学学报(自然科学版), 2021, 53(5): 17-22. doi: 10.6054/j.jscnun.2021070
引用本文: 张中岩, 刘诗莹, 许金松, 卢娉婷, 刘欣, 李煦田, 马立军. 基于苯并呋咱胺类衍生物的碱性荧光探针[J]. 华南师范大学学报(自然科学版), 2021, 53(5): 17-22. doi: 10.6054/j.jscnun.2021070
ZHANG Zhongyan, LIU Shiying, XU Jinsong, LU Pinting, LIU Xin, LI Xutian, MA Lijun. The PH Fluorescent Probe Based on Benzofuroxan Amine Derivatives[J]. Journal of South China normal University (Natural Science Edition), 2021, 53(5): 17-22. doi: 10.6054/j.jscnun.2021070
Citation: ZHANG Zhongyan, LIU Shiying, XU Jinsong, LU Pinting, LIU Xin, LI Xutian, MA Lijun. The PH Fluorescent Probe Based on Benzofuroxan Amine Derivatives[J]. Journal of South China normal University (Natural Science Edition), 2021, 53(5): 17-22. doi: 10.6054/j.jscnun.2021070

基于苯并呋咱胺类衍生物的碱性荧光探针

doi: 10.6054/j.jscnun.2021070
基金项目: 

广东省自然科学基金项目 2015A030313392

广州市科技计划项目 2018J2200342

详细信息
    通讯作者:

    马立军,Email: mlj898021@scnu.edu.cn

  • 中图分类号: O657.3

The PH Fluorescent Probe Based on Benzofuroxan Amine Derivatives

  • 摘要: 以4-氯-7-硝基苯并呋咱(NBD-Cl)为原料,设计、合成了3种荧光化合物(NBD-N、NBD-NH、NBD-NH2),通过质谱、核磁共振对其结构进行了表征. 研究表明:这3种化合物在pH 7.0~12.0范围内对水溶液的酸碱度具有可逆的荧光识别响应. 同时,其他常见离子的共存并不影响3种化合物对pH的荧光识别. 因此,这3种化合物可作为碱性pH荧光探针.
  • 图  1  化合物NBD-N、NBD-NH和NBD-NH2的合成线路图

    Figure  1.  The synthetic route for compound NBD-N, NBD-NH and NBD-NH2

    图  2  3种荧光探针(10.0 μmol/L)在不同pH水溶液中的紫外-可见吸收光谱

    Figure  2.  The absorption spectra of fluorescent probes (10.0 μmol/L) with different pH values

    图  3  3种荧光探针在不同pH缓冲溶液中的荧光发射光谱

    注:探针溶液、缓冲溶液浓度均为10.0 μmol/L,激发波长为465 nm.

    Figure  3.  The fluorescence spectra of three fluorescent probes in buffer solution with various pH values

    图  4  3种荧光探针的荧光发射峰强度与pH的关系

    Figure  4.  The intensity of the fluorescence emission peak of three fluorescent probes with different pH values

    图  5  3种荧光探针在不同离子缓冲溶液中的荧光发射光谱

    注:向pH为7.0的缓冲溶液中分别加入100.0 μmol/L的Fe3+、Co2+、Ni2+、Cu2+、Ag+、Zn2+、Hg2+、Mn2+、Cr3+、Al3+、Ca2+和Mg2+.

    Figure  5.  The fluorescence spectra of three fluorescent probes in buffer solution with different ions

    图  6  2种探针在碱性条件下的核磁共振谱及其对OH-的识别机理示意图

    Figure  6.  The NMR spectra of two probes under alkaline condition and their recognition mechanism for OH-

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出版历程
  • 收稿日期:  2020-09-07
  • 网络出版日期:  2021-11-11
  • 刊出日期:  2021-10-25

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