高璐, 黄瑞华, 张盛春. 拟南芥BLI基因调控植物衰老的研究[J]. 华南师范大学学报(自然科学版), 2021, 53(4): 49-54. doi: 10.6054/j.jscnun.2021057
引用本文: 高璐, 黄瑞华, 张盛春. 拟南芥BLI基因调控植物衰老的研究[J]. 华南师范大学学报(自然科学版), 2021, 53(4): 49-54. doi: 10.6054/j.jscnun.2021057
GAO Lu, HUANG Ruihua, ZHANG Shengchun. Regulation of Plant Senescence by BLI in Arabidopsis thaliana[J]. Journal of South China Normal University (Natural Science Edition), 2021, 53(4): 49-54. doi: 10.6054/j.jscnun.2021057
Citation: GAO Lu, HUANG Ruihua, ZHANG Shengchun. Regulation of Plant Senescence by BLI in Arabidopsis thaliana[J]. Journal of South China Normal University (Natural Science Edition), 2021, 53(4): 49-54. doi: 10.6054/j.jscnun.2021057

拟南芥BLI基因调控植物衰老的研究

Regulation of Plant Senescence by BLI in Arabidopsis thaliana

  • 摘要: BLISTER(BLI)蛋白是多种应激反应的调节因子,可以促进植物对环境的适应性. BLI蛋白通过抑制IRE1蛋白以维持植物的正常生长,缺失BLI蛋白的拟南芥植株出现多种发育缺陷表型. 文章以拟南芥bli突变体为实验材料,通过细胞生物学及遗传学方法检测其衰老相关表征,发现bli突变体植株早期出现叶片黄化、叶绿素含量下降、离子渗透率升高等一系列衰老表型;与此同时,bli突变体体内衰老相关基因表达上调,光合作用相关基因表达下调,表明BLI蛋白参与植物衰老过程;外源施加ROS导致野生型Col-0植株中BLI基因表达量减少; GUS显色结果表明BLI在植株各组织器官中均有表达,为进一步解析BLI调控衰老的分子网络提供了思路.

     

    Abstract: As a regulator of various stress responses, protein BLI promotes the adaptability of plants to the environment and maintains the normal growth of plants by inhibiting protein IRE1. Arabidopsis thaliana individuals without protein BLI are shown to have the mutant phenotype of pleiotropic development. The senescence-related characteristics of BLI mutant Arabidopsis thaliana plants were studied with the cell biology and genetic methods. It was found that a series of senescence phenotypes, such as leaf yellowing, decreased chlorophyll content and increased cell conductivity, appeared in the early stage of the BLI mutant plants and, at the same time, the expression of senescence-related genes in BLI mutant plants was up-regulated and the expression of photosynthesis-related genes was down-regulated. These results preliminarily proved that protein BLI was involved in plant senescence. Applying exogenous ROS to wild-type Col-0 plants showed that the BLI expression was reduced and GUS staining showed that BLI was expressed in all parts of the plant, which provides clues for further analyzing the molecular network for BLI regulating senescence.

     

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