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82种中草药及其复方对花鲈源维氏气单胞菌的体外抑菌效果

王宝屯 颜远义 毛灿 冯娟 邓益琴 苏友禄

王宝屯, 颜远义, 毛灿, 冯娟, 邓益琴, 苏友禄. 82种中草药及其复方对花鲈源维氏气单胞菌的体外抑菌效果[J]. 华南师范大学学报(自然科学版), 2021, 53(3): 64-72. doi: 10.6054/j.jscnun.2021043
引用本文: 王宝屯, 颜远义, 毛灿, 冯娟, 邓益琴, 苏友禄. 82种中草药及其复方对花鲈源维氏气单胞菌的体外抑菌效果[J]. 华南师范大学学报(自然科学版), 2021, 53(3): 64-72. doi: 10.6054/j.jscnun.2021043
WANG Baotun, YAN Yuanyi, MAO Can, FENG Juan, DENG Yiqin, SU Youlu. The Antibacterial Effect of 82 Kinds of Chinese Herbal Medicines and Their Compounds on Aeromonas vernoii from Lateolabrax maculatus in Vitro[J]. Journal of South China normal University (Natural Science Edition), 2021, 53(3): 64-72. doi: 10.6054/j.jscnun.2021043
Citation: WANG Baotun, YAN Yuanyi, MAO Can, FENG Juan, DENG Yiqin, SU Youlu. The Antibacterial Effect of 82 Kinds of Chinese Herbal Medicines and Their Compounds on Aeromonas vernoii from Lateolabrax maculatus in Vitro[J]. Journal of South China normal University (Natural Science Edition), 2021, 53(3): 64-72. doi: 10.6054/j.jscnun.2021043

82种中草药及其复方对花鲈源维氏气单胞菌的体外抑菌效果

doi: 10.6054/j.jscnun.2021043
基金项目: 

国家自然科学基金项目 31902415

中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助项目 2018ZD01

中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助项目 2019TS04

详细信息
    通讯作者:

    苏友禄,Email:youlusu@zhku.edu.cn

  • 中图分类号: S948

The Antibacterial Effect of 82 Kinds of Chinese Herbal Medicines and Their Compounds on Aeromonas vernoii from Lateolabrax maculatus in Vitro

  • 摘要: 为探究单方及复方中草药对花鲈(Lateolabrax maculatus)源维氏气单胞菌(Aeromonas vernoii)的体外抑菌作用,采用平板打孔法测定乌梅(Prunus mume)、苏木(Caesalpinia sappan)和五倍子(Galla chinensis)等82种单方中草药对维氏气单胞菌的抑制作用,用试管二倍稀释法测定抑菌作用较好的中草药对维氏气单胞菌最小抑菌质量浓度(Minimum Inhibitory Concentration, MIC)和最小杀菌质量浓度(Minimum Bactericidal Concentration, MBC),并选择更敏感的中药进行复方作用评价. 结果显示:36种中草药对维氏气单胞菌有抑制作用,其中乌梅、苏木、五倍子、丁香(Syzygium aromaticum)和艾叶(Artemisia argyi)对维氏气单胞菌抑制作用较强,抑菌圈直径介于15~25 mm,MIC < 125 mg/mL,且MBC < 500 mg/mL. 复方中草药中,乌梅+丁香、乌梅+五倍子对维氏气单胞菌抑制作用较好,抑菌圈直径>19 mm,MIC为15.6 mg/mL,MBC介于31.25~62.5 mg/mL,但联合抑菌指数分析显示它们呈现无关作用. 该研究表明:单方中草药乌梅、苏木有利于体外抑制和消除花鲈源维氏气单胞菌,为水产中草药物的开发提供数据支撑.
  • 表  1  供试中草药清单

    Table  1.   The list of Chinese herbal medicines in the experiment

    中草药 拉丁名 中草药 拉丁名
    乌梅 Prunus mume 白芍 Paeonia lactiflora
    苏木 Caesalpinia sappan 百合 Lilium brownii
    五倍子 Galla chinensis 槟榔 Areca catechu
    丁香 Syzygium aromaticum 赤芍 Paeoniae Radix
    艾叶 Artemisia argyi 穿心莲 Andrographis paniculata
    黄芩 Sastellaria baicalensis 淡竹叶 Lophatherum gracile
    石榴皮 Punica granatum 地骨皮 Lycium chinense
    五味子 Schisandra chinensis 浮萍 Lemna minor
    分心木 Juglans regia 甘草 Glycyrrhiza uralensis
    泽兰 Aconitum gymnandrum 谷芽 Setaria italica
    绵茵陈 Artemisia scoparia 虎杖 Polygonum cuspidatum
    小茴香 Nepeta tenuifolia 黄柏 Phellodendron chinense
    女贞子 Ligustrum lucidum 黄芪 Astragalus membranaceus
    八角茴香 Illicium verum 鸡骨草 Abrus precatorius
    青蒿 Artemisia annua 鸡内金 Galli Gigeriae
    仙鹤草 Agrimonia polosa 绞股蓝 Gynostemma pentaphyllum
    防风 Saposhnikovia divaricata 金银花 Lonicera japonica
    厚朴 Xylosma controversum 龙胆草 Gentiana scabra
    辛夷 Magnolia liliiflora 鹿含草 Pyrola calliantha
    荆芥 Nepeta cataria 络石藤 Trachelospermum jasminoides
    紫花地丁 Viola yedoensis 马齿苋 Portulaca oleracea
    苦地丁 Corydalis bungeana 麦芽 Hordeurn vulgare
    薄荷 Mentha haplocalyx 猫爪草 Ranunculus ternatus
    鱼腥草 Houttuynia cordata 牛蒡子 Arctium lappa
    大黄 Rheum palmatum 佩兰 Eupatorium fortunei
    板蓝根 Radix Isatidis 青黛 Indigo Naturalis
    野菊花 Dendranthema indicum 桑叶 Folium Mori
    蒲公英 Taraxacum mongolicum 神曲 Massa Medicata
    决明子 Cassia obtusifolia 柿蒂 Diospyros kaki
    白薇 Cynanchum atratum 天冬 Asparagus cochinchinensis
    合欢花 Albizia julibrissin 天花粉 Trichosanthes Kirilowii
    槐花 Sophora japonica 天南星 Arisaema erubescens
    苍术 Atractylodes lancea 吴茱萸 Evodia rutaecarpa
    石韦 Pyrrosia lingua 香加皮 Periploca sepium
    金钱草 Lysimachia christinae 延胡索 Corydalis yanhusuo
    栀子 Gardenia jasminoides 银柴胡 Stellaria dichotoma
    柏子仁 Platycladus orientalis 竹茹 Bambusa tuldoides
    桑寄生 Taxillus sutchuenensis 桂枝 Cinnamomum cassia
    桑枝 Morus alba 土牛膝 Achyranthes bidentata
    蔓荆 Vitex trifolia 红花 Carthamus tinctorius
    丹参 Salvia miltiorrhiza 白前 Cynanchum glaucescens
    下载: 导出CSV

    表  2  单方中草药对维氏气单胞菌体外抑菌效果

    Table  2.   The bacteriostatic effect of single Chinese herbal medicine on A. vernoii in vitro

    中草药 抑菌圈直径/mm 抑菌等级
    乌梅 Prunus mume 21.44±0.53 ++++
    苏木 Caesalpinia sappan 18.39±1.19 +++
    五倍子 Galla chinensis 18.17±1.87 +++
    丁香 Syzygium aromaticum 17.11±2.47 +++
    艾叶 Artemisia argyi 16.56±1.67 +++
    黄芩 Sastellaria baicalensis 14.89±1.36 ++
    石榴皮 Punica granatum 14.44±1.01 ++
    五味子 Schisandra chinensis 13.33±0.50 ++
    分心木 Juglans regia 12.78±1.06 ++
    泽兰 Aconitum gymnandrum 12.33±0.71 ++
    绵茵陈 Artemisia scoparia 12.17±0.61 ++
    小茴香 Nepeta tenuifolia 11.00±0.00 ++
    女贞子 Ligustrum lucidum 11.61±0.49 ++
    八角茴香 Illicium verum 11.39±0.22 ++
    青蒿 Artemisia annua 11.33±0.79 ++
    仙鹤草 Agrimonia polosa 10.00±0.43 ++
    防风 Saposhnikovia divaricata 10.00±0.43 ++
    厚朴 Xylosma controversum 10.89±1.02 ++
    辛夷 Magnolia liliiflora 10.78±0.26 ++
    荆芥 Nepeta cataria 10.67±0.87 ++
    紫花地丁 Viola yedoensis 10.56±1.13 ++
    苦地丁 Corydalis bungeana 10.22±0.67 ++
    薄荷 Mentha haplocalyx 10.22±0.57 ++
    鱼腥草 Houttuynia cordata 9.67±1.66 +
    大黄 Rheum palmatum 9.67±0.71 +
    板蓝根 Radix Isatidis 9.67±0.71 +
    野菊花 Dendranthema indicum 9.50±0.56 +
    蒲公英 Taraxacum mongolicum 9.22±0.91 +
    决明子 Cassia obtusifolia 9.11±0.60 +
    白薇 Cynanchum atratum 8.83±0.35 +
    合欢花 Albizia julibrissin 8.78±0.51 +
    槐花 Sophora japonica 8.72±0.67 +
    苍术 Atractylodes lancea 8.44±0.73 +
    石韦 Pyrrosia lingua 7.94±0.46 +
    金钱草 Lysimachia christinae 7.67±0.50 +
    栀子 Gardenia jasminoides 7.39±0.22 +
    注:++++为极度敏感;+++为高度敏感;++为中度敏感;+为低度敏感
    下载: 导出CSV

    表  3  7种复方中药对维氏气单胞菌体外抑菌效果

    Table  3.   The bacteriostatic effect of 7 kinds of compound Chinese herbal medicines on A. vernoii in vitro

    复方中草药 抑菌圈直径/mm 抑菌等级
    乌梅+丁香 Prunus mume+Syzygium aromaticum 22.50±0.58 ++++
    乌梅+五倍子 Prunus mume+ Galla chinensis 19.38±0.48 +++
    乌梅+苏木 Prunus mume+ Caesalpinia sappan 15.75±0.87 +++
    丁香+五倍子 Syzygium aromaticum+Galla chinensis 19.38±0.48 +++
    丁香+苏木 Syzygium aromaticum+Caesalpinia sappan 15.88±0.25 +++
    丁香+艾叶 Syzygium aromaticum+ Artemisia argyi 14.63±0.25 ++
    苏木+五倍子 Caesalpinia sappan+ Galla chinensis 17.75±0.50 +++
    注:++++为极度敏感;+++为高度敏感;++为中度敏感;+为低度敏感
    下载: 导出CSV

    表  4  单方中药对维氏气单胞菌最小抑菌浓度和最小杀菌浓度

    Table  4.   The minimum bacteriostatic concentration and minimum bactericidal concentration of single Chinese medicines on A. vernoii

    中草药 最小抑菌质量浓度/(mg·mL-1) 最小杀菌质量浓度/(mg·mL-1)
    乌梅 Prunus mume 15.60 31.25
    苏木 Caesalpinia sappan 15.60 31.25
    黄芩 Sastellaria baicalensis 15.60 15.60
    五倍子 Galla chinensis 31.25 31.25
    分心木 Juglans regia 31.25 62.50
    绵茵陈 Artemisia scoparia 31.25 31.25
    丁香 Syzygium aromaticum 62.50 125.00
    石榴皮 Punica granatum 62.50 125.00
    五味子 Schisandra chinensis 62.50 62.50
    八角茴香 Illicium verum 62.50 62.50
    青蒿 Artemisia annua 62.50 125.00
    辛夷 Magnolia liliiflora 62.50 125.00
    艾叶 Artemisia argyi 125.00 500.00
    荆芥 Nepeta cataria 125.00 250.00
    紫花地丁 Viola yedoensis 125.00 125.00
    苦地丁 Corydalis bungeana 125.00 250.00
    薄荷 Mentha haplocalyx 125.00 250.00
    小茴香 Nepeta tenuifolia 125.00 125.00
    仙鹤草 Agrimonia polosa 125.00 125.00
    防风 Saposhnikovia divaricata 250.00 500.00
    厚朴 Xylosma controversum 250.00 250.00
    泽兰 Aconitum gymnandrum 500.00 500.00
    女贞子 Ligustrum lucidum 500.00 500.00
    下载: 导出CSV

    表  5  复方中草药对维氏气单胞菌最小抑菌质量浓度和最小杀菌质量浓度

    Table  5.   The minimum bacteriostatic concentration and minimum bactericidal concentration of compound Chinese herbal medicines on A. vernoii

    中草药 最小抑菌质量浓度/ (mg·mL-1) 最小杀菌质量浓度/ (mg·mL-1) 联合抑菌指数 作用效应
    乌梅+苏木 Prunus mume+Caesalpinia sappan 31.25 62.50 4.00 拮抗
    乌梅+五倍子 Prunus mume+Galla chinensis 15.60 31.25 1.50 无关
    乌梅+丁香 Prunus mume+Syzygium aromaticum 15.60 62.50 1.20 无关
    苏木+五倍子 Caesalpinia sappan+Galla chinensis 31.25 62.50 3.00 拮抗
    苏木+丁香 Caesalpinia sappan+Syzygium aromaticum 62.50 125.00 5.00 拮抗
    五倍子+丁香 Galla chinensis+Syzygium aromaticum 31.25 62.50 1.50 无关
    艾叶+丁香 Artemisia argyi+Syzygium aromaticum 31.25 125.00 0.75 相加
    下载: 导出CSV
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出版历程
  • 收稿日期:  2020-06-29
  • 网络出版日期:  2021-07-06
  • 刊出日期:  2021-06-25

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