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一种可富集Fe3+的含芘荧光探针

王莎 黄子杰 肖盈希 卢博 唐健 马立军

王莎, 黄子杰, 肖盈希, 卢博, 唐健, 马立军. 一种可富集Fe3+的含芘荧光探针[J]. 华南师范大学学报(自然科学版), 2020, 52(6): 34-38. doi: 10.6054/j.jscnun.2020091
引用本文: 王莎, 黄子杰, 肖盈希, 卢博, 唐健, 马立军. 一种可富集Fe3+的含芘荧光探针[J]. 华南师范大学学报(自然科学版), 2020, 52(6): 34-38. doi: 10.6054/j.jscnun.2020091
WANG Sha, HUANG Zijie, XIAO Yingxi, LU Bo, TANG Jian, MA Lijun. A Pyrene-containing Fluorescent Probe with Enriching Fe3+[J]. Journal of South China normal University (Natural Science Edition), 2020, 52(6): 34-38. doi: 10.6054/j.jscnun.2020091
Citation: WANG Sha, HUANG Zijie, XIAO Yingxi, LU Bo, TANG Jian, MA Lijun. A Pyrene-containing Fluorescent Probe with Enriching Fe3+[J]. Journal of South China normal University (Natural Science Edition), 2020, 52(6): 34-38. doi: 10.6054/j.jscnun.2020091

一种可富集Fe3+的含芘荧光探针

doi: 10.6054/j.jscnun.2020091
基金项目: 

广东省自然科学基金项目 2015A030313392

广州市科技计划项目 201804010465

详细信息
    通讯作者:

    马立军,副教授,Email:mlj898021@scnu.edu.cn

  • 中图分类号: O65

A Pyrene-containing Fluorescent Probe with Enriching Fe3+

  • 摘要: 以芘为荧光发射基团、以邻氨基苯甲酸基团为识别基团,设计、合成了一种对Fe3+特异性响应的荧光探针芘甲酰邻氨基苯甲酸(PAA).利用氢核磁共振波谱、质谱表征其结构,通过紫外-可见吸收光谱以及荧光发射光谱研究其对Fe3+的响应性能.结果表明:PAA对Fe3+具有高选择性、高抗干扰性的荧光响应.随着Fe3+浓度的升高,探针的荧光强度逐渐减弱,同时伴随大量Fe3+螯合物沉淀的析出,从而使Fe3+从体系中富集出来,且在4倍浓度干扰离子存在的体系中,PAA对Fe3+的识别能力几乎不受影响.因此,PAA在实际环境应用中,具有较大的潜力和应用价值.
  • 图  1  PAA的合成路线

    Figure  1.  The synthesis route of PAA

    图  2  PAA的1H NMR及质谱图

    Figure  2.  The 1H NMR and ESI-MS spectra of PAA

    图  3  PAA溶液中加入不同浓度Fe3+时的紫外-可见吸收光谱和荧光发射光谱

    注:图B中插图为在波长为365 nm的紫外灯照射下,探针分子PAA对Fe3+响应的照片,其中a溶液c(PAA)=20.0 μmol/L, b溶液中c(PAA)=20.0 μmol/L,c(Fe3+)=120.0 μmol/L.

    Figure  3.  The fluorescence emission spectra and UV-Vis spectra of PAA upon addition of various amounts of Fe3+

    图  4  不同金属离子浓度对PAA荧光发射峰强度的影响及其干扰性

    注:图A中c(PAA)=20.0 μmol/L, 发射峰中心波长为495 nm.图B为在PAA(20.0 μmol/L)水溶液中,单独添加Fe3+浓度(30.0 μmol/L)及同时添加Fe3+(30.0 μmol/L)和竞争离子(150.0 μmol/L)时溶液荧光发射强度的相对变化.

    Figure  4.  The effects of different metal ion concentrations on the intensity of PAA fluorescence emission peaks and their interference

    图  5  不同离子探针比例对PAA荧光发射峰强度的影响及其在最佳结合模式下的拟合曲线

    注:图A中PAA与Fe3+在水溶液中的总浓度为20.0 μmol/L; 最佳结合模式n(PAA) : n(Fe3+)=2:1.

    Figure  5.  The effect of different ion probe ratios on the intensity of PAA fluorescence emission and its fitting in the best binding mode

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出版历程
  • 收稿日期:  2019-09-25
  • 刊出日期:  2020-12-25

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