Abstract: A fluorescent probe (pyrene-formyl-anthranilic acid, PAA) with specific response to Fe³⁺ was designed and synthesized using pyrene as a fluorescent group and anthranilic acid group as a receptor. The structure was characterized with hydrogen nuclear magnetic resonance spectroscopy and mass spectrometry. Visible absorption spectra and fluorescence emission spectra were used to study the response of Fe³⁺. The results showed that PAA had high selectivity and high anti-interference fluorescence response to Fe³⁺. With the increase of Fe³⁺ concentration, the probe's fluorescence intensity was gradually weakened, and a large amount of iron ion chelate precipitated and so that it could be removed from the system. In the system where 4 times of interfering ions existed, the ability of PAA to recognize Fe³⁺ was hardly affected. Therefore PAA has a great potential for practical application.