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基于苯并呋咱的HSO3-离子荧光探针

冯锐鸿 曾惠文 陈志昕 吕足 马立军

冯锐鸿, 曾惠文, 陈志昕, 吕足, 马立军. 基于苯并呋咱的HSO3-离子荧光探针[J]. 华南师范大学学报(自然科学版), 2020, 52(4): 57-62. doi: 10.6054/j.jscnun.2020061
引用本文: 冯锐鸿, 曾惠文, 陈志昕, 吕足, 马立军. 基于苯并呋咱的HSO3-离子荧光探针[J]. 华南师范大学学报(自然科学版), 2020, 52(4): 57-62. doi: 10.6054/j.jscnun.2020061
FENG Ruihong, ZENG Huiwen, CHEN Zhixin, LÜ Zu, MA Lijun. A Fluorescence Probe for HSO3- Based on Benzofurazan[J]. Journal of South China normal University (Natural Science Edition), 2020, 52(4): 57-62. doi: 10.6054/j.jscnun.2020061
Citation: FENG Ruihong, ZENG Huiwen, CHEN Zhixin, LÜ Zu, MA Lijun. A Fluorescence Probe for HSO3- Based on Benzofurazan[J]. Journal of South China normal University (Natural Science Edition), 2020, 52(4): 57-62. doi: 10.6054/j.jscnun.2020061

基于苯并呋咱的HSO3-离子荧光探针

doi: 10.6054/j.jscnun.2020061
基金项目: 

广州市科技计划项目 2018J2200342

详细信息
    通讯作者:

    马立军,副教授,Email:mlj898021@scnu.edu.cn

  • 中图分类号: O657.3

A Fluorescence Probe for HSO3- Based on Benzofurazan

  • 摘要: 以4-氯-7-硝基苯并呋咱(NBD-Cl)和咪唑-4-甲醛为原料,设计合成了一种用于检测亚硫酸氢根离子(HSO3-)的荧光探针,并通过核磁共振氢谱(1H-NMR)、质谱(MS)对其结构进行了表征.荧光测试结果表明:在二甲基亚砜(DMSO)水溶液中,该化合物能够对HSO3-表现出荧光增强响应,而相同条件下该化合物对其他阴离子几乎没有类似的荧光识别现象,因此该荧光化合物对HSO3-具有专一荧光识别作用,是一种有效的HSO3-荧光探针.
  • 图  1  NBD-NH-NH2与NBQ的合成路线

    Figure  1.  The route of NBD-NH-NH2 and NBQ synthesis

    图  2  向探针NBQ的溶液中加入不同浓度HSO3-溶液后的荧光发射光谱

    注:NBQ溶液的浓度为20.0 μmol/L,溶剂V(DMSO)/V(H2O)=1/4.滴定后溶液中HSO3-的浓度为0.0~100.0 μmol/L.测试的激发波长为370 nm.插图为未加入(左)和加入100.0 μmol/L HSO3-后(右)探针NBQ在波长为365 nm的紫外光照射下荧光的变化.

    Figure  2.  The fluorescent spectra of NBQ upon addition of HSO3- solution

    图  3  向NBQ中分别加入HSO3-和其他阴离子后溶液的荧光光谱

    注:NBQ溶液的浓度为20.0 μmol/L,溶剂V(DMSO)/V(H2O)=1/4. HSO3-和其他阴离子溶液的浓度均为100.0 μmol/L.测试的激发波长为370 nm.

    Figure  3.  The fluorescent spectra of NBQ solution upon addition of HSO3- and other anions

    图  4  向NBQ溶液中同时加入HSO3-和其他干扰阴离子后荧光峰强度的变化

    注:NBQ溶液的浓度为20.0 μmol/L,溶剂V(DMSO)/V(H2O)=1/4. HSO3-和其他阴离子溶液的浓度分别为20.0、100.0 μmol/L.发射峰的中心波长为574 nm.

    Figure  4.  The emission intensities of NBQ solution upon addition of HSO3- mixed with other interring anions

    图  5  NBQ与HSO3-的Job's曲线

    Figure  5.  The Job's plot of binding constant for NBQ and HSO3-

    图  6  NBQ与HSO3-的结合常数拟合以及检测灵敏度

    Figure  6.  The fitting of binding constant between NBQ and HSO3- and the detection sensitivity

    图  7  向NBQ溶液中加入不同浓度HSO3-的紫外-可见光谱

    注:NBQ溶液的浓度为20.0 μmol/L,溶剂V(DMSO)/V(H2O)=1/4. HSO3-的浓度为0.0~45.0 μmol/L.插图为未加入(左)和加入45.0 μmol/L HSO3-后(右)探针NBQ溶液颜色的变化.

    Figure  7.  The UV-Vis absorption spectra of NBQ upon addition of HSO3-

    图  8  不同pH对探针NBQ缓冲溶液在加入HSO3-后的荧光强度变化

    注:NBQ溶液的浓度为20.0 μmol/L. HSO3-的浓度为20.0 μmol/L.

    Figure  8.  The fluorescence intensity change of HSO3- added to probe NBQ buffer solution at different pH values

    图  9  探针NBQ与HSO3-的结合示意图

    Figure  9.  The potential model of the response of probe NBQ to HSO3-

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  • 收稿日期:  2019-09-23
  • 刊出日期:  2020-08-25

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