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基因重组发光菌应用于环境样品毒性的测试

方贵桢 胡立新 黄国勇 赵建亮 应光国

方贵桢, 胡立新, 黄国勇, 赵建亮, 应光国. 基因重组发光菌应用于环境样品毒性的测试[J]. 华南师范大学学报(自然科学版), 2020, 52(2): 60-67. doi: 10.6054/j.jscnun.2020028
引用本文: 方贵桢, 胡立新, 黄国勇, 赵建亮, 应光国. 基因重组发光菌应用于环境样品毒性的测试[J]. 华南师范大学学报(自然科学版), 2020, 52(2): 60-67. doi: 10.6054/j.jscnun.2020028
FANG Guizhen, HU Lixin, HUANG Guoyong, ZHAO Jianliang, YING Guangguo. The Application of Gene Recombinant Luminescent Bacteria to Environmental Sample Toxicity Test[J]. Journal of South China normal University (Natural Science Edition), 2020, 52(2): 60-67. doi: 10.6054/j.jscnun.2020028
Citation: FANG Guizhen, HU Lixin, HUANG Guoyong, ZHAO Jianliang, YING Guangguo. The Application of Gene Recombinant Luminescent Bacteria to Environmental Sample Toxicity Test[J]. Journal of South China normal University (Natural Science Edition), 2020, 52(2): 60-67. doi: 10.6054/j.jscnun.2020028

基因重组发光菌应用于环境样品毒性的测试

doi: 10.6054/j.jscnun.2020028
基金项目: 

广东省应用型科技研发专项 2015B020235012

详细信息
    通讯作者:

    胡立新,博士,Email:lixin.hu@m.scnu.edu.cn

    应光国,研究员,Email:guangguo.ying@m.scnu.edu.cn

  • 中图分类号: O43; Q89

The Application of Gene Recombinant Luminescent Bacteria to Environmental Sample Toxicity Test

  • 摘要: 选取基因重组发光菌E.coli HB101 pUCD607和费氏弧菌为试验生物,采用急性毒性微孔板法,发光抑制率为测试指标,分别用锌离子、汞离子作为阳性对照,研究8种重金属分别对基因重组发光菌和费氏弧菌产生的生物毒性,结果显示:锌离子毒性小、稳定性高、变异系数小,更适合作为阳性对照.利用基因重组发光菌比较了离子液体和有机溶剂的生物毒性效应,同等条件下的毒性测试结果表明:基因重组发光菌半数效应质量浓度(EC50)比费氏弧菌高,灵敏度更高;在铜离子、锰离子、铁离子和镉离子的毒性作用下,作为受试生物,基因重组发光菌的灵敏度显著高于费氏弧菌(EC50之间存在显著性差异);离子液体对基因重组发光菌的毒性显著高于有机溶剂.综上所述,基因重组发光菌应用于环境样品的毒性检测可行性较高.
  • 图  1  阳性对照物质锌离子对基因重组发光菌的毒性效应

    Figure  1.  The toxic effects of positive control substance zinc ions on gen recombinant luminescent bacteria

    图  2  8种重金属对基因重组发光菌和费氏弧菌的毒性比较

    Figure  2.  The comparison of the toxicity of eight heavy metals to gene recombinant luminescent bacteria and Vibrio fischeri

    图  3  6种有机溶剂对基因重组发光菌的剂量-效应曲线

    Figure  3.  The dose-effect curve of six organic solvents to gene recombinant luminescent bacteria

    图  4  6种不同烷基链长度离子液体对基因重组发光菌的剂量-效应曲线

    Figure  4.  Theose-effect curve of six different alkyl chain length ionic liquids to gene recombinant luminescent bacteria

    图  5  有机溶剂和离子液体对基因重组发光菌的毒性比较

    Figure  5.  The comparison of toxicity of organic solvents and ionic liquids to gene recombinant luminescent bacteria

    表  1  8种重金属对费氏弧菌和基因重组发光菌的剂量-效应曲线拟合信息

    Table  1.   The dose-effect curve fitting information of eight heavy metals on Vibrio fischeri and gene recombinant luminescent bacteria

    重金属化合物 菌种类型 模型 a b c EC50/(mg·L-1) R2
    ZnSO4 费氏弧菌 Boltzmann Sigmoidal -0.148 0.801 0.803 1.978 0.962
    重组发光菌 0.055 0.929 -2.745 1.203 0.993
    CuSO4 费氏弧菌 Boltzmann Sigmoidal 0.020 0.891 5.798 17.860 0.995
    重组发光菌 -0.078 0.909 0.376 1.646 0.996
    FeCl3 费氏弧菌 Boltzmann Sigmoidal -0.095 0.988 1.780 5.214 0.999
    重组发光菌 -0.115 0.875 0.135 1.226 0.995
    HgCl2 费氏弧菌 Boltzmann Sigmoidal -0.136 0.996 0.055 0.150 0.999
    重组发光菌 0.023 0.983 0.006 0.659 0.999
    MnCl2 费氏弧菌 Boltzmann Sigmoidal -0.004 0.985 10.680 47.370 1.000
    重组发光菌 -0.001 0.922 4.945 9.935 0.995
    CdCl2 费氏弧菌 Boltzmann Sigmoidal -0.464 1.011 22.070 18.290 0.987
    重组发光菌 -0.084 1.058 -0.815 8.718 0.978
    CoCl2 费氏弧菌 Boltzmann Sigmoidal 0.050 0.819 4.744 2.898 0.989
    重组发光菌 -0.204 1.272 -0.676 1.045 0.987
    K2Cr2O7 费氏弧菌 Boltzmann Sigmoidal -0.605 1.009 8.431 11.980 0.983
    重组发光菌 0.036 0.970 1.715 10.550 0.999
    注:abc分别表示Boltzmann Sigmoidal的拟合参数,EC50表示半数效应质量浓度,R2表示相关系数的平方.
    下载: 导出CSV

    表  2  6种有机溶剂对基因重组发光菌的剂量-效应曲线拟合信息

    Table  2.   The dose-effect curve fitting information of six organic solvents on gene recombinant luminescent bacteria

    有机溶剂 模型 ZEC/(g·L-1) EC/(g·L-1) EC50/(g·L-1) R2
    甲醇 LSSVM 10.110 2.319 36.120 0.985
    乙醇 LSSVM 54.050 9.246 52.140 1.000
    丙酮 LSSVM 26.050 4.687 10.270 0.992
    二甲基亚砜 LSSVM 122.500 12.890 101.800 0.999
    甲醛 Boltzmann Sigmoidal 4.168 1.194 5.122 0.995
    乙腈 LSSVM 38.700 11.510 47.880 0.985
    注:ZEC表示零效应点质量浓度,EC表示最大刺激效应点质量浓度,EC50表示半数效应质量浓度,R2表示相关系数的平方,表 3同.
    下载: 导出CSV

    表  3  6种离子液体对基因重组发光菌的剂量-效应曲线拟合信息

    Table  3.   The dose-effect curve fitting information of six ionic liquids on gene recombinant luminescent bacteria

    离子液体 模型 ZEC/(g·L-1) EC/(g·L-1) EC50/(g·L-1) R2
    IM-2 LSSVM 22.010 4.687 20.780 0.999
    IM-4 Boltzmann Sigmoidal - - 9.131 0.996
    IM-6 Boltzmann Sigmoidal - - 0.219 0.992
    IM-8 Boltzmann Sigmoidal - - 0.012 0.988
    IM-10 Boltzmann Sigmoidal - - 0.024 0.982
    IM-12 Boltzmann Sigmoidal - - 0.016 0.997
    下载: 导出CSV
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  • 收稿日期:  2019-09-15
  • 刊出日期:  2020-04-25

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