留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

斜纹夜蛾精巢的发育和精子的发生

文靓 陈丰 陈亚青 周来娣 刘玉成 冯启理 刘琳

文靓, 陈丰, 陈亚青, 周来娣, 刘玉成, 冯启理, 刘琳. 斜纹夜蛾精巢的发育和精子的发生[J]. 华南师范大学学报(自然科学版), 2019, 51(4): 47-56. doi: 10.6054/j.jscnun.2019065
引用本文: 文靓, 陈丰, 陈亚青, 周来娣, 刘玉成, 冯启理, 刘琳. 斜纹夜蛾精巢的发育和精子的发生[J]. 华南师范大学学报(自然科学版), 2019, 51(4): 47-56. doi: 10.6054/j.jscnun.2019065
WEN Liang, CHEN Feng, CHEN Yaqing, ZHOU Laidi, LIU Yucheng, FENG Qili, LIU Lin. The Testis Development and Spermatogenesis in Spodoptera litura (Lepidoptera: Noctuidae)[J]. Journal of South China normal University (Natural Science Edition), 2019, 51(4): 47-56. doi: 10.6054/j.jscnun.2019065
Citation: WEN Liang, CHEN Feng, CHEN Yaqing, ZHOU Laidi, LIU Yucheng, FENG Qili, LIU Lin. The Testis Development and Spermatogenesis in Spodoptera litura (Lepidoptera: Noctuidae)[J]. Journal of South China normal University (Natural Science Edition), 2019, 51(4): 47-56. doi: 10.6054/j.jscnun.2019065

斜纹夜蛾精巢的发育和精子的发生

doi: 10.6054/j.jscnun.2019065
基金项目: 

国家自然科学基金项目 31330071

国家自然科学基金项目 31772519

广东省自然科学基金项目 2017A030313210

详细信息
    通讯作者:

    刘琳,副教授,Email:liul@scnu.edu.cn

  • 中图分类号: Q963

The Testis Development and Spermatogenesis in Spodoptera litura (Lepidoptera: Noctuidae)

  • 摘要: 采用解剖观察、石蜡组织切片及苏木精-曙红染色方法,利用显微镜照相获取图像,研究了斜纹夜蛾幼虫到成虫精巢的发育及精子的发生.研究结果表明:2个精巢在预蛹期开始融合,并在蛹期融合成1个;在成虫期,随着精子的排出,精巢体积逐渐减小;斜纹夜蛾雄性生殖细胞发育为有核精子和无核精子,2种精子的发生都经过精原细胞囊期、精母细胞囊期和精细胞囊期;精子的形成主要区别在于有核精子的细胞核有一个变形过程,而无核精子形成过程中不经过细胞核变形,且后期将细胞核丢弃.综上所述,有核精子的主要发育时期为6龄到蛹期,而无核精子的主要发育在蛹期.本研究结果可为进一步研究和控制精子形成以及利用生殖机理防治斜纹夜蛾打下基础.
  • 图  1  斜纹夜蛾雌雄的鉴别

    a:第7腹节;b:第8腹节;c:第9腹节;d:雌性生殖孔;e:雄性生殖孔;f:第10腹节;g:肛门;红色方框为斜纹夜蛾雄性幼虫生殖附腺;黑色箭头示雌性成虫圆孔状腹部末端;红色箭头示雄性成虫二裂片状腹部末端.

    Figure  1.  The identification of male and female Spodoptera litura

    图  2  斜纹夜蛾5龄和6龄幼虫精巢的长宽径变化

    注:L5D1~L5D3表示5龄第1~3天,L6D1~L6D6表示6龄第1~6天,柱形图表示精巢的长宽径在5个重复中的平均值,不同字母表示各组差异具有统计意义(P < 0.05).

    Figure  2.  The changes of long and wide diameter of testis in the 5 th and 6 th instar larvae of Spodoptera litura

    图  3  斜纹夜蛾5龄和6龄幼虫精巢发育的动态变化

    A~C:分别为5龄幼虫第1~3天精巢;D~I:分别为6龄幼虫第1~6天精巢.其中黑色箭头示系带,白色箭头示气管.

    Figure  3.  Dynamic changes of testis development in the 5 th and 6 th instar larvae of Spodoptera litura

    图  4  斜纹夜蛾蛹期精巢发育的动态变化

    Figure  4.  Dynamic changes of testis development in the pupa of Spodoptera litura

    图  5  斜纹夜蛾蛹期精巢的长宽径变化

    注:P1~P11表示蛹期的第1~11天,柱形图表示精巢的长宽径在5个重复中的平均值,不同字母表示各组差异具有统计学意义(P < 0.05).

    Figure  5.  The changes of long and wide diameter of testis in the pupa of Spodoptera litura

    图  6  斜纹夜蛾成虫精巢的长宽径变化

    注:A1~A10表示成虫期的第1~10天,柱形图表示成虫精巢的长宽径在5个重复中的平均值,不同字母表示各组差具有统计学意义(P < 0.05).

    Figure  6.  The changes of long and wide diameter of testis in the adult of Spodoptera litura

    图  7  斜纹夜蛾成虫精巢发育的动态变化

    Figure  7.  Dynamic changes of testis development in the adult of Spodoptera litura

    图  8  斜纹夜蛾有核精子发生的各阶段

    A:精原细胞囊期(5龄第3天),示意精原细胞囊(spc);B:初级精母细胞囊期和次级精母细胞囊期(5龄第3天),初级精母细胞囊(psc)中央无空腔,次级精母细胞囊(ssc)中央有较大空腔;C:精细胞囊期(6龄第3天),精细胞囊(sc)中央有大型空腔,精细胞(s)间隔明显. as:顶端干细胞; sp:精原细胞;cc:囊细胞.

    Figure  8.  The stages of eupyrene spermatogenesis in Spodoptera litura

    图  9  斜纹夜蛾无核精子发生各时期

    A:精原细胞囊期(6龄第2天),精原细胞(sp)由若干囊细胞(cc)包围形成精原细胞囊(spc);B:精母细胞囊期和精细胞囊期(6龄第4天),精母细胞囊(scc)中央有小型空腔,精细胞囊(sc)无空腔,细胞排列均匀.

    Figure  9.  The stages of apyrene spermatogenesis in Spodoptera litura

    图  10  斜纹夜蛾有核精子形成各阶段

    A:Ⅰ阶段(6龄第3天),精细胞(s)变形拉长成三角形,细胞核(N)聚集在精细胞囊前端;B:Ⅱ阶段(6龄第6天),精囊伸长呈圆柱形,细胞核排列在前端;C:Ⅲ阶段(蛹期第1天),精囊前端突起,细胞核呈椭圆形; D~F:Ⅳ阶段;D:蛹期第3天,精细胞拉长;E:蛹期第3天,细胞核线状,红色箭头示拉长的精囊前端突起;F:蛹期第3天,精子束尾部波浪状;G~H:Ⅴ阶段;G:蛹期第3天,精子束排出多余细胞质;H:蛹期第4天;I:Ⅵ阶段(蛹期第7天),成熟精子束(msb). hcc:顶端囊细胞;wb:废物袋;d:废弃物.

    Figure  10.  The stages of eupyrene spermiogenesis in Spodoptera litura

    图  11  斜纹夜蛾无核精子形成各阶段

    A:Ⅰ阶段(蛹期第3天),细胞核(N)均匀分布在精囊中;B:Ⅱ阶段(蛹期第4天),精囊两端伸长,细胞核聚集在囊中间;C:Ⅲ阶段(蛹期第6天),精囊两端继续拉长,形成无核精子束(asb);D:Ⅳ阶段(蛹期第6天),精子束行蠕动;E:Ⅴ阶段(蛹期第6天),成熟精子束(msb). cc:囊细胞;wb:废物袋;d:废弃物.

    Figure  11.  The stages of apyrene spermiogenesis in Spodoptera litura

    图  12  斜纹夜蛾精子的发生和形成过程示意图

    A:精原细胞囊;B:初级精母细胞囊;C:次级精母细胞囊;D:有核精细胞囊;E:有核精子Ⅰ阶段;F:有核精子Ⅱ阶段;G:有核精子Ⅲ阶段;H:有核精子Ⅳ阶段;I:有核精子Ⅴ阶段;J:成熟有核精子束;K:精原细胞囊;L:无核精母细胞囊;M:无核精细胞囊;N:无核精子Ⅰ阶段;O:无核精子Ⅱ阶段;P:无核精子Ⅲ阶段;Q:无核精子Ⅳ阶段;R:成熟无核精子束.

    Figure  12.  The diagram of the process of spermatogenesis and spermiogenesis in Spodoptera litura

  • [1] ETMAN A A M, HOOPER G H S. Developmental and reproductive biology of Spodoptera litura (F.) (Lepidoptera:Noctuidae)[J]. Austral Entomology, 1979, 18(4):363-372. doi: 10.1111-j.1440-6055.1979.tb00868.x/
    [2] 赵卓, 郝锡联, 李娜, 等.四平地区6种蝗虫精巢和卵巢发育动态[J].吉林农业大学学报, 2008, 30(5):677-681. http://d.old.wanfangdata.com.cn/Periodical/jlnydxxb200805007

    ZHAO Z, HAO X L, LI N, et al. The dynamic development of spermary and ovary of six species of dominant locusts in Siping area of Jilin Province, China[J]. Journal of Jilin Agricultural University, 2008, 30(5):677-681. http://d.old.wanfangdata.com.cn/Periodical/jlnydxxb200805007
    [3] KLOWDEN M J. Physiological systems in insects[M]. Second edition. America:Academic Press, 2013:149-196.
    [4] SMITH J L, BAUTE T S, SEBRIGHT M M, et al. Establishment of Striacosta albicosta (Lepidoptera:Noctuidae) as a primary pest of corn in the great lakes region[J]. Journal of Economic Entomology, 2018, 111(4):1732-1744. doi: 10.1093/jee/toy138
    [5] ZHANG Y, DONG Z, GU P, et al. Proteomics analysis of adult testis from Bombyx mori [J]. Proteomics, 2014, 14(20):2345-2349. doi: 10.1002/pmic.201300507
    [6] BILHA J K, BRANCALHAO R M C, CONTE H. Testicles fusion of Diatraea saccharalis F. (Lepidoptera; Crambidae) during post-embryonic development[J]. Journal of the Entomological Research Society, 2012, 14(3):1-7. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=fcc46a77bad36fefd685317856b8ebcf
    [7] MARI I P, GIGLIOLLI A, NANYA S, et al. Histological and electron microscopy observations on the testis and spermatogenesis of the butterfly Dione juno (Cramer, 1779) and Agraulis vanillae (Linnaeus, 1758) (Lepidoptera:Nymphalidae)[J]. Micron, 2018, 109:11-21. doi: 10.1016/j.micron.2018.03.004
    [8] PEREIRA M C, SANTOS D C. Testis morphology and dichotomic spermatogenesis in the sugarcane borer Diatraea saccharalis (F.) (Lepidoptera:Crambidae)[J]. Neotropical Entomology, 2015, 44(5):481-488. doi: 10.1007/s13744-015-0312-7
    [9] FRIEDLANDER M, SETH R K, REYNOLDS S E. Eupyrene and apyrene sperm:dichotomous spermatogenesis in lepidoptera[J]. Advances in Insect Physiology, 2005, 32:206-308. doi: 10.1016/S0065-2806(05)32003-0
    [10] MANCINI K, DOLDER H. Sperm morphology and arrangement along the male reproductive tract of the butterfly Euptoieta hegesia (Insecta:Lepidoptera)[J]. International Journal of Invertebrate Reproduction, 2003, 44(2/3):107-117. doi: 10.1080/07924259.2003.9652561
    [11] MANCINI K, DOLDER H. Ultrastructure of apyrene and eupyrene spermatozoa from the seminal vesicle of Euptoieta hegesia (Lepidoptera:Nymphalidae)[J]. Tissue & Cell, 2001, 33(3):301-308. https://www.sciencedirect.com/science/article/abs/pii/S0040816601901843
    [12] ALVES L, MANCINI K, LINO-NETO J, et al. Morphology of the male reproductive system and sperm ultrastructure of Leucoptera coffeella (Lepidoptera :Lyonetiidae)[J]. Acta Zoologica, 2006, 87(2):131-139. doi: 10.1111/j.1463-6395.2006.00226.x
    [13] TUAN S J, YEH C C, ATLIHAN R, et al. Demography and consumption of Spodoptera litura (Lepidoptera:Noctuidae) reared on cabbage and taro[J]. Journal of Economic Entomology, 2016, 109(2):732-739. doi: 10.1093/jee/tov325
    [14] KUMAR A N, MURUGAN K, MADHIYAZHAGAN P. Integration of botanicals and microbials for management of crop and human pests[J]. Parasitology Research, 2013, 112(1):313-325. doi: 10.1007/s00436-012-3139-8
    [15] MEHRDAD A, BAHAREH S, ADLY A A, et al. Feasibility of using the radiation-based sterile insect technique (SIT) to control the olive fruit fly, Bactrocera oleae, Gmelin (Diptera:Tephritidae) in Iran[J]. Applied Radiation and Isotopes, 2018, 139:279-284. doi: 10.1016/j.apradiso.2018.05.032
    [16] 廖德贵, 赖妙玲, 唐甜, 等.免疫组化抗体的选择与优化对报告结果的影响[J].热带医学杂志, 2016, 16(9):1126-1128. http://d.old.wanfangdata.com.cn/Periodical/rdyxzz201609011

    LIAO D G, LAI M L, TANG T, et al. Impact of the immunohistochemical antibody selection and optimization on the clinical report[J]. Journal of Tropical Medicine, 2016, 16(9):1126-1128. http://d.old.wanfangdata.com.cn/Periodical/rdyxzz201609011
    [17] FRIEDLANDER M, HAUSCHTECK-JUNGEN E. Differential basic nucleoprotein kinetics in the two kinds of lepidoptera spermatids:Nucleate (eupyrene) and anucleate (apyrene)[J]. Chromosoma, 1982, 85(3):387-398. doi: 10.1007/BF00330361
    [18] 吴德余.桑蚕的生活习性以及生态特征[J].吉林农业, 2010(2):54-55. doi: 10.3969/j.issn.1674-0432.2010.02.045

    WU D Y. Habits and ecological characteristics of silkworm[J]. Agriculture of Jilin, 2010 (2):54-55. doi: 10.3969/j.issn.1674-0432.2010.02.045
    [19] 孙少轩.斑蜡蝉生殖系统的解剖与组织学[J].昆虫学报, 1966, 15(4):303-314.

    SUN S X. On the anatomy and histology of the reproductive system of Lycorma delicatula White[J]. Acta Entomologica Sinica, 1966, 15(4):303-314.
    [20] 陈大福, 牛宝龙, 翁宏飚, 等.棉铃虫(Helicoverpa armigera)幼虫性特征和精子的发生[J].浙江农业学报, 2004, 16(6):354-357. doi: 10.3969/j.issn.1004-1524.2004.06.002

    CHEN D F, NIU B L, WENG H B, et al. Study on larval sexual character and spermatogenesis of cotton boll worm (Helicoverpa armigera)[J]. Acta Agriculturae Zhejiangensis, 2004, 16(6):354-357. doi: 10.3969/j.issn.1004-1524.2004.06.002
    [21] 刘绪生.棉铃虫精子行为研究[D].南京: 南京农业大学, 2001.

    LIU X S. Behavior of sperm in cotton Bollworm, Helicoverpa armigera[D]. Nanjing: Nanjing Agricultural University, 2001.
    [22] 金朝阳, 丁兆兵, 龚和.昆虫雄性生殖附腺生理功能及其生殖因子研究进展[J].科学通报, 2001, 46(14):1151-1155. doi: 10.3321/j.issn:0023-074X.2001.14.002
    [23] MANCINI K, DOLDER H. Dichotomic spermiogenesis in Euptoieta hegesia (Lepidoptera:nymphalidae)[J]. Brazilian Journal of Morphological Science, 2004, 21(1):13-23.
    [24] DUBROVSKY E B. Hormonal cross talk in insect development[J]. Trends in Endocrinology & Metabolism, 2005, 16(1):6-11. http://cn.bing.com/academic/profile?id=cc49d04d3db9dfa8f3e4bc793c570780&encoded=0&v=paper_preview&mkt=zh-cn
    [25] ANNEKATRIN K, YATSENKO A S, WEISS M, et al. Ecdysteroids affect Drosophila ovarian stem cell niche formation and early germline differentiation[J]. Embo Journal, 2011, 30(8):1549-1562. doi: 10.1038/emboj.2011.73
    [26] MORRIS L X, SPRADLING A C, et al. Steroid signaling within Drosophila ovarian epithelial cells sex-specifically modulates early germ cell development and meiotic entry[J]. PloS One, 2012, 7(10):e46109/1-11. http://d.old.wanfangdata.com.cn/OAPaper/oai_pubmedcentral.nih.gov_3462805
    [27] LI Y J, MA Q, CHERRY C M, et al. Steroid signaling promotes stem cell maintenance in the Drosophila testis[J]. Developmental Biology, 2014, 394(1):129-141. doi: 10.1016/j.ydbio.2014.07.016
    [28] 叶恭银, 胡萃, 龚和.保幼激素和蜕皮激素对天蚕卵巢发育的影响[J].浙江农业大学学报, 1999, 25(3):276-380. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zjdxxb-nyysm199903016

    YE G Y, HU C, GONG H. Effects of juvenile hormone and ecdysteroid on ovarian development of the Japanese oak silkworm Antheraea yamamai [J]. Journal of Zhejiang Agricultural University, 1999, 25(3):276-380. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=zjdxxb-nyysm199903016
    [29] 周丽娜.保幼激素对昆虫生长发育和生殖的影响[J].上海农业科技, 2017(2):131-133. doi: 10.3969/j.issn.1001-0106.2017.02.081
    [30] KAWAMURA N, YAMASHIKI N, BANDO H. Behavior of mitochondria during eupyrene and apyrene spermatogenesis in the silkworm, Bombyx mori (Lepidoptera), investigated by fluorescence in situ hybridization and electron microscopy[J]. Protoplasma, 1998, 202(3/4):223-231. http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=e4ddc140d97040eebdec89a1eda12e13
    [31] POLANSKA M A, CIUK M A, CYMBOROWSKI B, et al. Germ cell death in the testis and its relation to spermatogenesis in the wax moth, Galleria mellonella (Lepidoptera:Pyralidae), effects of facultative diapause[J]. Journal of Experimental Zoology, 2005, 303A(11):1013-1029. doi: 10.1002/jez.a.227
    [32] KAWAMURA N, SAITOH H S L, YAMASHIKI N. Peristaltic squeezing of sperm bundles at the late stage of spermatogenesis in the silkworm, Bombyx mori[J]. Journal of Morphology, 2000, 246(2):53-58. doi: 10.1002/1097-4687(200011)246:2<53::AID-JMOR1>3.0.CO;2-P
    [33] 钦俊德.昆虫生理学研究进展[M].北京:科学出版社, 1981.
  • 加载中
图(12)
计量
  • 文章访问数:  2196
  • HTML全文浏览量:  819
  • PDF下载量:  73
  • 被引次数: 0
出版历程
  • 收稿日期:  2019-04-10
  • 刊出日期:  2019-08-25

目录

    /

    返回文章
    返回