为研究长期施用粪肥土壤中抗生素耐药基因的消减规律，分别采集长期施用与未施用粪肥农田中的土壤，进行土壤培养对照实验，探究土壤含水率及生物质炭的添加对土壤中可移动元件及耐药基因在310 d内的丰度变化的影响. 结果表明，长期施用粪肥提高了耐药基因与可移动元件在土壤中的污染水平. 长期施用粪肥土壤在实验室培养条件下，耐药基因与可移动元件呈现指数消减的趋势. 添加生物质炭的土壤中耐药基因和可移动元件消减较慢，含水率较高的土壤中耐药基因和可移动元件消减较快. 可移动元件相对丰度可一定程度上反映土壤培养过程中耐药基因的消减. 实验结果揭示了受污染农田土壤中耐药基因的动态变化规律，可为土壤耐药基因污染风险评估提供基础数据，为我国抗生素耐药性防控提供科学参考.
For study the dynamics of antibiotic resistance genes (ARGs) in soil chronically fertilized by swine manure, a soil incubation experiment was conducted. The dynamic changes of ARGs and mobile genetic elements (MGEs) were investigated within 310 days. The impacts of soil water content and addition of biochar was studied. The results showed that long-term manure fertilization contributed to significant increase of ARGs and MGEs in soil. In soil chronically fertilized with manure amended with different treatments, ARGs and MGEs decreased exponentially with incubation time. The degration of ARGs and MGEs in soil was slower with added biochar. Higher water content could significantly enhance the degration of ARGs and MGEs. The significant correlation between ARGs and MGEs indicated that MGEs can serve as an indicator of ARG reduction in soil. The results revealed the dynamic changes of ARGs in contaminated farmland soils induced by the application of swine manure, providing basic data for risk assessment of ARG contamination in soils and scientific reference for antibiotic resistance control in China.