留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

斜纹夜蛾几丁质酶家族基因鉴定与时空表达分析

张晓娟 丁洋 陈亚青 黄立华 郑思春

张晓娟, 丁洋, 陈亚青, 黄立华, 郑思春. 斜纹夜蛾几丁质酶家族基因鉴定与时空表达分析[J]. 华南师范大学学报(自然科学版), 2015, 47(3): 100-0. doi: 10.6054/j.jscnun.2015.01.005
引用本文: 张晓娟, 丁洋, 陈亚青, 黄立华, 郑思春. 斜纹夜蛾几丁质酶家族基因鉴定与时空表达分析[J]. 华南师范大学学报(自然科学版), 2015, 47(3): 100-0. doi: 10.6054/j.jscnun.2015.01.005
Identification and Expression Analysis OF Chitinases in Common Cutworm, Spodoptera Litura[J]. Journal of South China normal University (Natural Science Edition), 2015, 47(3): 100-0. doi: 10.6054/j.jscnun.2015.01.005
Citation: Identification and Expression Analysis OF Chitinases in Common Cutworm, Spodoptera Litura[J]. Journal of South China normal University (Natural Science Edition), 2015, 47(3): 100-0. doi: 10.6054/j.jscnun.2015.01.005

斜纹夜蛾几丁质酶家族基因鉴定与时空表达分析

doi: 10.6054/j.jscnun.2015.01.005
基金项目: 

科技部十二五农村领域科技计划项目

详细信息
    通讯作者:

    张晓娟

Identification and Expression Analysis OF Chitinases in Common Cutworm, Spodoptera Litura

  • 摘要: 昆虫GH18家族几丁质酶属于多基因家族,在昆虫的蜕皮与变态过程中发挥着至关重要的作用,它催化表皮几丁质的降解.斜纹夜蛾是一种重要的农业害虫,危害290多种植物,但对其几丁质酶未深入研究.本文通过斜纹夜蛾转录组学分析,发现了12种潜在的几丁质酶,分属于8类.对这些几丁质酶在斜纹夜蛾变态发育时在表皮、中肠以及脂肪体的时空表达分析结果表明,SlCht5, SlCht9和SlCht2的表达水平在幼虫转化为蛹时上调表达,说明这些几丁质酶可能参与了斜纹夜蛾变态时期的脱皮作用;而其他几丁质酶的表达具有组织特性,说明它们可能有着不同的功能,如SlCht6仅在中肠和脂肪体中表达,SlCht11,SlCht12 和 SlCht16主要在中肠和表皮表达,SlIDGF2仅在表皮有表达,SlIDGF3和SlCht9在三大组织均有表达.本研究对斜纹夜蛾几丁质酶家族基因的鉴定和时空表达作了初步的分析,为研究它们在斜纹夜蛾发育中的功能与调控打下基础.
  • [1] Kramer KJ, Muthukrishnan S.Insect chitinases:molecular biology and potential use as biopesticides[J].[J].InsectBiochem.Mol.Biol., 1997, 27(11):887-900 [2] Zhang J, Zhang X, Arakane Y, Muthukrishnan S, Kramer KJ, et al.Comparative Genomic Analysisof Chitinase and Chitinase-Like Genes in the African Malaria Mosquito (Anopheles gambiae)[J].PLoS ONE, 2011, 6(5):- [3]Shi L, Paskewitz S M.Identification and molecular characterization of two immune-responsive chitinase-like proteins from Anopheles gambiae[J].Insect Mol Biol, 2004, 13(4):387-398 [4]Takahashi M, Kiuchi M, Kamimura M.A new chitinase-related gene,BmChiR1,is induced in the Bombyx mori anterior silk gland at molt and metamorphosis by ecdysteroid[J].Insect Biochem Mol Biol, 2002, 32(2):147-151 [5] Huang SJ and Han ZJ.Mechanisms for multiple resistances in field populations of common cutworm, Spodoptera litura (Fabricius)in China [J][J].Pestic Biochem Physiol, 2007, 87:14-22 [6] Ahmad M, Saleem MA and Sayyed AH.Efficacy of insectic idemixtures against pyrethroid and organophos phate-resistant populations of Spodoptera litura (Lepidoptera:Noctuidae)[J[J].Pest Manage Sci, 2009, (65):266-274 [7] Shinoda T, Kobayashi J, Matsui M, Chinzei Y.Cloning and functional expression of a chitinase cDNA from the common cutworm, Spodoptera?litura, using a recombinant baculovirus lacking the virus-encoded?chitinase gene[J].[J].Insect Biochem Mol Biol, 2001, 31(6-7):521-532 [8]Zhu QS, Arakane Y, Beeman RW.Functional specialization among insect chitinase family genes revealed by RNA interference[J][J].PNAS, 2008, 105(18):6650-6655 [9]Gu, J, Huang, L.X., Gong, Y.J., Zheng, S.C., Liu, L., Huang, L.H.*, Feng, Q.L.. De novo characterization of transcriptome and gene expression dynamics in epidermis during the larval-pupal metamorphosis of common cutworm[J]. [J].Insect Biochem. Mol. Biol., 2013, 43:794-808 [10]Zhu QS, Arakane Y, Debarshi B.Domain organization and phylogenetic analysis of the chitinase-like family of proteins in three species of insects[J][J]..Insect Biochem.Mol.Biol., 2008, 38(4):45-46 [11] de la Vega H, Specht CA, Liu Y, Robbins PW.Chitinases are a multigene family in Aedes, Anopheles and Drosophila[J].1998, 7:233–239.[J].Insect Mol Biol., 1998, 7:233-239 [12]Genta F.A.,Blanes L,Cristofoletti P. T. Purification,characterization and molecular cloning of the major chitinase fromTenebrio molitor larval midgut[J].Insect Biochem.Mol.Biol., 2006, 36(10):789-800 [13]Fitches E.Wilkinson H,Bell H. Cloning,expression and functional characterization of tomato moth (Lacanobia oleracea): a demonstration of the insecticidal activity of insect chitinase[J].Insect Biochem.Mol.Biol., 2004, 34(10):1037-1050 [14]Bade ML, Wyatt GR.Metabolic conversions during pupation of The cecropia silkworm1.Deposition and utilization of nutrient reserves[J].Biochem J, 1962, 83(3):470-478 [15] Zhu Q, Arakane Y, Banerjee D, Beeman RW, Kramer KJ, Muthukrishnan S.Domain organization and phylogenetic analysis of the chitinase-like family of proteins in three species of insects[J[J].Insect Biochem Mol Biol, 2008, 38(4):452-466 [16] Arakane Y, Zhu QS, Matsumiya M, et al.Properties of catalytic, linker and chitin-binding domains of insect chitinase[J]., 2003(33):631-648[J].Insect Biochemistry and Molecular Biology, 2003, 33:631-648 [17] Yasuyuki Arakane, Subbaratnam Muthukrishnan.Insect chitinase and chitinase-like proteins[J].Cell[J].Mol.Life Sci, 2010, 67:201-216 [18] Kramer, KJ., Corpuz, L., Choi, H.K., .Muthukrishnan, S.Sequence of a cDNA and expression of the gene encoding epidermal and gut chitinases of Manduca sexta.[J].Insect Biochem.Mol.Biol., 1993, 23:691-701

    [1] Kramer KJ, Muthukrishnan S.Insect chitinases:molecular biology and potential use as biopesticides[J].[J].InsectBiochem.Mol.Biol., 1997, 27(11):887-900 [2] Zhang J, Zhang X, Arakane Y, Muthukrishnan S, Kramer KJ, et al.Comparative Genomic Analysisof Chitinase and Chitinase-Like Genes in the African Malaria Mosquito (Anopheles gambiae)[J].PLoS ONE, 2011, 6(5):- [3]Shi L, Paskewitz S M.Identification and molecular characterization of two immune-responsive chitinase-like proteins from Anopheles gambiae[J].Insect Mol Biol, 2004, 13(4):387-398 [4]Takahashi M, Kiuchi M, Kamimura M.A new chitinase-related gene,BmChiR1,is induced in the Bombyx mori anterior silk gland at molt and metamorphosis by ecdysteroid[J].Insect Biochem Mol Biol, 2002, 32(2):147-151 [5] Huang SJ and Han ZJ.Mechanisms for multiple resistances in field populations of common cutworm, Spodoptera litura (Fabricius)in China [J][J].Pestic Biochem Physiol, 2007, 87:14-22 [6] Ahmad M, Saleem MA and Sayyed AH.Efficacy of insectic idemixtures against pyrethroid and organophos phate-resistant populations of Spodoptera litura (Lepidoptera:Noctuidae)[J[J].Pest Manage Sci, 2009, (65):266-274 [7] Shinoda T, Kobayashi J, Matsui M, Chinzei Y.Cloning and functional expression of a chitinase cDNA from the common cutworm, Spodoptera?litura, using a recombinant baculovirus lacking the virus-encoded?chitinase gene[J].[J].Insect Biochem Mol Biol, 2001, 31(6-7):521-532 [8]Zhu QS, Arakane Y, Beeman RW.Functional specialization among insect chitinase family genes revealed by RNA interference[J][J].PNAS, 2008, 105(18):6650-6655 [9]Gu, J, Huang, L.X., Gong, Y.J., Zheng, S.C., Liu, L., Huang, L.H.*, Feng, Q.L.. De novo characterization of transcriptome and gene expression dynamics in epidermis during the larval-pupal metamorphosis of common cutworm[J]. [J].Insect Biochem. Mol. Biol., 2013, 43:794-808 [10]Zhu QS, Arakane Y, Debarshi B.Domain organization and phylogenetic analysis of the chitinase-like family of proteins in three species of insects[J][J]..Insect Biochem.Mol.Biol., 2008, 38(4):45-46 [11] de la Vega H, Specht CA, Liu Y, Robbins PW.Chitinases are a multigene family in Aedes, Anopheles and Drosophila[J].1998, 7:233–239.[J].Insect Mol Biol., 1998, 7:233-239 [12]Genta F.A.,Blanes L,Cristofoletti P. T. Purification,characterization and molecular cloning of the major chitinase fromTenebrio molitor larval midgut[J].Insect Biochem.Mol.Biol., 2006, 36(10):789-800 [13]Fitches E.Wilkinson H,Bell H. Cloning,expression and functional characterization of tomato moth (Lacanobia oleracea): a demonstration of the insecticidal activity of insect chitinase[J].Insect Biochem.Mol.Biol., 2004, 34(10):1037-1050 [14]Bade ML, Wyatt GR.Metabolic conversions during pupation of The cecropia silkworm1.Deposition and utilization of nutrient reserves[J].Biochem J, 1962, 83(3):470-478 [15] Zhu Q, Arakane Y, Banerjee D, Beeman RW, Kramer KJ, Muthukrishnan S.Domain organization and phylogenetic analysis of the chitinase-like family of proteins in three species of insects[J[J].Insect Biochem Mol Biol, 2008, 38(4):452-466 [16] Arakane Y, Zhu QS, Matsumiya M, et al.Properties of catalytic, linker and chitin-binding domains of insect chitinase[J]., 2003(33):631-648[J].Insect Biochemistry and Molecular Biology, 2003, 33:631-648 [17] Yasuyuki Arakane, Subbaratnam Muthukrishnan.Insect chitinase and chitinase-like proteins[J].Cell[J].Mol.Life Sci, 2010, 67:201-216 [18] Kramer, KJ., Corpuz, L., Choi, H.K., .Muthukrishnan, S.Sequence of a cDNA and expression of the gene encoding epidermal and gut chitinases of Manduca sexta.[J].Insect Biochem.Mol.Biol., 1993, 23:691-701
  • 加载中
计量
  • 文章访问数:  1265
  • HTML全文浏览量:  91
  • PDF下载量:  208
  • 被引次数: 0
出版历程
  • 收稿日期:  2015-01-05
  • 修回日期:  2015-02-02
  • 刊出日期:  2015-05-25

目录

    /

    返回文章
    返回