The Changes of endogenous hormones during early gravitropic response of peanut（Arachis hynpogaea. L） gynophore
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摘要: 花生果针离体培养试验表明：不论果针以什么角度插入无激素的MS培养基，离体培养6h后，其尖端均发生弯曲(垂直向下除外)呈向地生长趋势.采用HPLC的方法，比较正置和倒置果针离体培养6h和12h果针主要内源激素的变化，结果显示：正置果针在离体生长过程中GA3，IAA和CTK的质量分数逐渐下降，而倒置果针在离体生长过程中IAA质量分数变化不大，但GA3和CTK质量分数显著增加；与此相反，ABA的含量在正置培养果针离体生长过程中是逐渐增加，而倒置培养是逐渐减少.说明这些植物内源激素在果针的向地性弯曲过程中起到了重要作用.Abstract: When peanut gynophores were grown in vitro on MS medium, all gynophore tips planted at different angles showed gravitropic curvature at 6 h of culture, except for the gynophore with tip pointing downward. The change of endogenous hormones in peanut gynophores planted with tip pointing upward or downward morphologically in vitro was investigated by high performance liquid chromatography (HPLC). The results showed that the contents of IAA, GA3 and CTK decreased gradually in gynophores planted with tip pointing downward. IAA showed little change at all stages, and the contents of GA3 and CTK increased significantly in gynophores planted with tip pointing upward. On the contrary, the content of ABA increased gradually in gynophores planted with tip pointing downward, but decreased in the upward gynophores. The results indicated that these endogenous hormones might play an important role in gravitropic curvature of peanut gynorphores.
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