The disease development and defense - related biochemical changes in the leave of Anthurium andraenum cultivar Alabamb were assayed after inoculation with Xanthomonas axonopodis ABB-2. Pathogenicity assay revealed thatAlabambwas highly sensitive to X. axonopodis. On the day 4 in infection experiment of detached leaf and on the day 8 in infection experiment of pot plant with the X. axonopodis, all inoculated leaves developed disease symptoms of bacterial blight, and the percentage of leaves with lesions reached 100%. The activities of four tested defense - related enzymes，SOD, POD, PPO, and PAL in the leave of Anthurium Alabamb increased and reached the peaks on days 4, 6, 8 and 12 after inoculation with X. axonopodis ABB-2, with the increase values of 15.37, 36.62, 46.68, and 66.43%, respectively, when compared to those of non- inoculated control plant. After the peaks, SOD and POD activities had a continuous decrease until the level of lower than that of the non- inoculated control plant. And PPO activity followed by a decrease to the level close to that of the control plant. Whereas PAL activity have only a slight decrease. After inoculation with X. axonopodis ABB-2, soluble protein content and free phenolic content in the leave of A. andraenum Alabambincreased by 21.76 and 34.66%, respectively, compared to those of non- inoculated control plant.