16S rDNA序列技术分析鉴定颗粒污泥中的微生物

Application Of 16S rDNA Ribosomal RNA Approach For The Analysis And Identification of Bacterium In Granular Sludge

摘要: 采用16S rDNA序列分析技术对降解五氯酚的微氧颗粒污泥形成过程中真细菌和古细菌的种群多样性和动态变化进行了研究.通过对DGGE主要条带进行序列比对，发现颗粒污泥中真细菌和古细菌都与不可培养的微生物具有很高的相似性，微氧颗粒污泥中同时存在好氧菌、微氧菌和厌氧菌.通过比较不同菌的相对数量变化发现五氯酚驯化后的颗粒污泥中产生了一系列对五氯酚降解有利的优势细菌和古菌，如Proteobacteria、Sphingomonas、Methanogenic bacterium等.

Abstract: Both Eubacteria and Archaea community diversity and dynamicity were studied during PCP-degrading micro-aerobic granular sludge cultivation using 16S rDNA ribosomal RNA approach. Main bands of DGGE were compared with sequences. The results showed that Eubacteria and Archaea community were closely related to uncultured microorganisms. Compared the relative quantity change of different bacteria, there were aerobic, micro-aerobic and anaerobic bacterium in micro-aerobic granular sludge simultaneously. After granular sludge was domesticated by PCP, the dominant Eubacteria and Archaea for PCP degradation were related to Proteobacteria, Sphingomonas, Methanogenic bacterium and so on.