Abstract: The chromosomal karyotypes of three common varieties of Gerbera jamesonii hybr were analyzed using the conventional root-tip squashing technology and their ploidy detection was carried out by flow cytometry. The results show that the three varieties of Gerbera jamesonii hybr tested are all diploid. The Shenzhen No.5 had a karyotype formula of2n=2x=50=2M+34m+16sm, the chromosome relative length index of2n=6L+12M2+24M1+8S, and the asymmetry coefficient ofAS.K% = 60.09%, which belongs to the2Ctype according to Stebbins standard karyotype classification. The Xiangbin had a karyotype formula of2n=2x=50+B=36m+12sm+2st+B, the chromosome relative length index of2n=8L+10M2+16M1+16S, and the asymmetry coefficient ofAS.K% =62.17%, which belongs to the2Ctype. The Datoufen had a karyotype formula of2n=2x=50+B=34m+12sm+4st+B, the chromosome relative length index of2n=8L+8M2+28M1+6S, and the asymmetry coefficient ofAS.K% =62.21%, which belongs to the2Btype. In conclusion, the three varieties of Gerbera jamesonii hybr were different in chromosome length, position of centromere, B chromosome and karyotype.