Abstract: An aerobic mixed culture strains CDB that can degrad Cefradine were enriched from wastewater discharge port. CDB could can use Cefradine as sole carbon source for growth. 3 pure single strains were isolated and cultured. The 4d degradation test in 0，5，10，15，20，25mgL-1 Cefradine were done and results showed that the all of the bacterial densities reached the peak after 4 days and slowed down when cultured in 10-25mgL-1 Cefradine. Results also showed that in the test of the degradation ability in 10mgL-1 Cefradine , the density of the mixed culture strains CDB had the highest degradation ability than the single strains. In the 3 single strains, the CDB3 had higher bacterium density and higher degradation ability than the other 2 stains. Strain which grow well in the 10mgL-1 Cefradine has higher degradation ability.