Abstract: This article investigates the long-term inhibitory effect of co-immobilized IFN- and TNF- on the growth of HeLa cells. IFN- and TNF- were co-immobilized on cell culture polystyrene and cells were incubated 18d on that. Cell counting was used to determine the inhibitory rate of co-immobilized cytokines on HeLa cells. Inverted microscope was used to observe the cellular form. The analysis for the exposure of phosphatidylserine was used to testify the apoptosis. The study showed that the co-immobilized IFN- and TNF-（20ng/well）could inhibit HeLa cells growth in a time-dependant manner, reached 94.12% inhibitory rate at day 18 and had significant activity of inducing HeLa cells apoptosis. The analysis for the exposure of PS showed that the apoptosis rates reached 76.6% and 88.7% after being cultured 3 and 6 days.