双酚A对河蚬呼吸代谢和抗氧化酶的毒性研究

Toxicity studies of BPA on oxygen consumption rate and antioxidant enzymes in Asian calms

摘要: 采用静态染毒法，研究了双酚A(bisphenol A，BPA)对河蚬(Corbicula fluminea)的急性毒性和BPA在1/2 LC50, 1/4 LC50, 1/8 LC50, 1/10 LC50亚急性浓度胁迫下耗氧率和排氨率以及抗氧化酶系统中 SOD、CAT活性的变化，以探究河蚬在BPA胁迫下的生物学响应.结果显示：BPA对河蚬96 h-LC50为6.34 mg·L-1；亚急性毒性指标耗氧率、排氨率以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）对BPA比较敏感，在本试验浓度范围内（0.63 ～3.17 mg·L-1）河蚬耗氧率、排氨率以及两种酶活性变化均呈现先下降后上升再下降的趋势，且有较好的一致性和规律性.结果表明，BPA对河蚬产生了氧化损伤，具有一定的毒性作用.

Abstract: The acute toxicities of bisphenol A (BPA ) to the Asian clams (Corbicula fluminea) and the toxic effects of BPA on the oxygen consumption rate (OCR), the ammonia excretory rate (AER), and the activity of antioxidant enzymes including superoxide dismutase (SOD) and catalase (CAT) in the clams at the concentrations of 1/2 LC50, 1/4 LC50, 1/8 LC50, 1/10 LC50 of BPA were investigated. The results showed that the 96 h-LC50 of BPA to the clams was 6.34 mg·L-1. The sensitive response of OCR, AER, SOD and CAT of the clams to BPA exposure in the range of 0.63 mg·L-1~3.17 mg·L-1 showed a typical pattern. This may indicate that BPA had oxidative stress and toxic effects on the growth of Asian clams.