为了解我国睑虎属Goniurosaurus (Squamata: Sauria: Eublepharidae)种间的亲缘关系以及广东新纪录的睑虎属未定种Goniurosaurus indet.的有效性, 我们对睑虎属10个种的线粒体DNA基因片段进行比较和遗传变异分析. 获得12S rRNA (385 bp)和16S rRNA (477 bp)总长度为862 bp的联合序列, 共有核苷酸变异位点388个, 简约信息位点269个. 选取大壁虎Gekko gecko为外类群, 采用贝叶斯演绎法(BI)、最大似然法(ML)、最大简约法(MP)、邻接法(NJ)对两个基因联合序列的分析均显示相同结果, 即广东睑虎属未定种Goniurosaurus indet.与英德睑虎G. yingdeensis以及凭祥睑虎G .luii与蛛纹睑虎G. araneus两两种间的亲缘关系分别较近; 且分布区相邻的Goniurosaurus indet.与G. yingdeensis之间的遗传分化(遗传距离=0.044-0.046)相似于分类地位已经明确的G .luii与G. araneus种间的遗传差异(遗传距离=0.051). 结合前人的形态学研究结果, 我们认为Goniurosaurus indet.和G. yingdeensis之间已发生显著的遗传差异, 前者可能为睑虎属新种. 同时, 系统发育分析表明海南睑虎G. hainanensis与睑虎属其它物种之间的遗传分化较大, 系统发育地位最为特殊. 系统发育分析进一步表明具4条背部横纹是祖征, 具5条背部横纹为衍征. 我们的研究补充了睑虎科的基础资料, 为该属的系统发育研究和物种保护提供了一定的理论依据. 本文首次提出宽阔的河流可能是造成睑虎种群分化和新种形成的重要地理屏障. 这一“河流隔离”观点对全球睑虎属物种的亲缘地理分布格局的解释具有重要的理论意义.
We studied the genetic differentiation of Goniurosaurus indet. from northwestern Guangdong Province, China, and its phylogenetic relationships with other Chinese species, using partial sequences of the mitochondrial 12S rRNA and 16S rRNA genes of 10 species in total and Gekko gecko as the outgroup. A total sequence of 862 nucleotides of 12S rRNA (385 bp) and 16S rRNA (477 bp) genes were analyzed; 388 variable sites and 123 parsimoniously informative sites were detected in combination of the two genes. Analyses of Bayesian inference (BI), maximum likelihood (ML), maximum parsimony (MP), and neighbor-joining (NJ) all showed similar results: a close phylogenetic relationship between Goniurosaurus indet. and G. yingdeensis (genetic distance = 0.044-0.046), comparable to that between accepted known species G. araneus and G. luii (genetic distance = 0.051). Combined with morphological differences, our results support the notion that there is significant genetic differentiation between Goniurosaurus indet. and G. yingdeensis even though the two forms are separated only by a river; thus, Goniurosaurus indet. may be a new species. The phylogenetic analyses also indicated the uniqueness of G. hainanensis with the greatest genetic distance from others, suggesting that the character state of four body bands including nuchal loop is plesiomorphic as compared to that of five body bands. Our study lends support to the known phylogeny of Eublepharidae and provides complementary data for the conservation of Goniurosaurus. We hypothesized for the first time that rivers act as isolatiing barrier for population differentiation and speciation among continental Goniurosaurus species.