用高效液相色谱法（HPLC）建立黑米指纹图谱的分析方法.采用Diamomnsil C18色谱柱（4.6250㎜， 5m），流动相为甲醇-甲酸（体积分数为0.5%）-乙腈，三元台阶梯度洗脱，流速0.6mL/min，柱温30℃，检测波长为254 nm.以液质联用法确定内参比峰，通过谱图分析和相对保留时间的计算，确定了13个黑米品种的8个共有峰；以平均矢量法计算出13个指纹图谱的共有模式,并以此作为对照图谱,再计算所有样品的相似度，各黑米品种指纹图谱相似度良好（0.9）。该法为比较分析各品种黑米提供了有效的微观信息，同时也为科学评价黑米质量及新品种培育提供了检测方法.
Fingerprints of 13 samples of black rice were studied by high-performance liquid chromatography (HPLC). The gradient elution mode was applied in chromatographic separation. Dikma Technologies Diamomnsil C18 (250mm46mm, 5m) was used with the mobile phase: 05%(v/v)formic acid water solution-acetonitrile-methanol, flow rate: 06 mL/min, detecting wavelength: 254 nm and the column temperature: 30℃. By calculating the relative retention times of certain peaks with the centre peak as the reference standard which has the great absorption, 8 common peaks in the samples were analyzed. Relative retention times for the common peaks of various samples were calculated, and the similarities of all the samples were figured out through each peak area by using the vectorial angle cosine method and correlative coefficient method. The results indicated that each black rice sample which have similary color, have a good similarity, w ith the similarity greater than 0.934. This method had a good repeatability and reproducibility, and can be used to identify and evaluate the quality of black rice conveniently.