用高糖（25mmol/L葡萄糖）和胆固醇（0.8, 1.6, 3.2mmol/L）处理EA.分析高糖高胆固醇诱导EA.hy926细胞凋亡中对HSPD1基因表达的影响，研究表明随胆固醇浓度的增加，EA.hy926细胞活性下降，呈浓度依赖性.0.8mmol/L胆固醇低糖培养液培养12h的细胞活性与正常对照组无显著差异（P>0.05），而0.8mmol/L胆固醇高糖培养液培养12h的细胞活性与正常对照组（高糖培养液+0mmol/L胆固醇）具显著性差异(P<0.01).低糖或高糖培养液+0.8mmol/L胆固醇处理EA.hy926细胞不同时间后，细胞活性呈时间依赖性下调，48h后细胞大部分变圆，脱落.高糖高胆固醇处理的细胞大部分发生凋亡（50%），HSPD1基因表达显著上调.
To explore the expression of HDPD1 gene in EA.hy926 cell apoptosis induced by high glucose and hight cholesterol. Methods: cells were incubated with high or low concentrations of glucose (25 mmol/L ) and cholestrol (0.8 , 1.6 ,3.2 mmol/L) for different times. We detected the cell activity by CCK8 Kit, observed the cell apoptosis by FACS (Flow Cytometer), and measured the expression of HSPD1 gene by RT-PCR and QPCR. Results: Cholesterol (0.8 , 1.6 , 3.2 mmol/L) together with high glucose strikingly decresed cell activity and in a dose-dependent manner, the same as the cholesterol (1.6 , 3.2 mmol/L) together with low glucose, but not for the cholestrol (0.8 mmol/L) with low glucose. The cell activity also decreased in a time dependent manner when treated with low or high glucose + 0.8 mmol/L. Most of cells treated with hight glucose and hight cholesterol (high glucose + 0.8 mmol/L cholesterol) underwent apoptosis compared with the low glucose and high cholesterol (high glucose + 0.8 mmol/L cholesterol). Meanwhile, the expression of HSPD1 gene were dramatically induced by high glucose and high cholesterol.